Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cells
Objective(s): Breast cancer cells developing radioresistance during radiation may result in cancer recurrence and poor survival. One of the main reasons for this problem is the changes in the regulation of genes that have a key role in the epithelial-mesenchymal transition (EMT). Utilizing mesenchym...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-04-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_21785_43be12477e5bd2aecf7b09a6e627ac67.pdf |
| _version_ | 1797848991576096768 |
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| author | Rahil Ghanbarnasab Behbahani Amir Danyaei Hamed Shoghi Mohammad Javad Tahmasbi Ghasem Saki Niloofar Neisi |
| author_facet | Rahil Ghanbarnasab Behbahani Amir Danyaei Hamed Shoghi Mohammad Javad Tahmasbi Ghasem Saki Niloofar Neisi |
| author_sort | Rahil Ghanbarnasab Behbahani |
| collection | DOAJ |
| description | Objective(s): Breast cancer cells developing radioresistance during radiation may result in cancer recurrence and poor survival. One of the main reasons for this problem is the changes in the regulation of genes that have a key role in the epithelial-mesenchymal transition (EMT). Utilizing mesenchymal stem cells can be an effective approach to overcome therapeutic resistance. In this study, we investigated the possibility of combining mesenchymal medium with cancer cell medium in sensitizing breast carcinoma cells to radiation. Materials and Methods: In this experimental study, the cells were irradiated at a dose of 4 Gy alone and in combination with stem cells and cancer cells media. Apoptosis, cell cycle, Western blotting, and real-time PCR assays evaluated the therapeutic effects. Results: We found that the CSCM could decrease the expression of several EMT markers (CD133, CD44, Vimentin, Nanog, Snail, and Twist), resulting in increased cell distribution in the G1 and G2/M phases, apoptosis rate, and protein levels of p-Chk2 and cyclin D1; furthermore, it exhibits synergetic effects with radiation treatment in vitro. Conclusion: These findings show that CSCM inhibits the expansion of breast cancer cells and makes them more susceptible to radiotherapy, offering a unique approach to treating breast cancer by overcoming radioresistance. |
| first_indexed | 2024-04-09T18:36:36Z |
| format | Article |
| id | doaj.art-ae6c0d583b2e48f1b702179db28d4724 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-09T18:36:36Z |
| publishDate | 2023-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ae6c0d583b2e48f1b702179db28d47242023-04-11T09:41:55ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-04-0126448649110.22038/ijbms.2023.68374.1491921785Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cellsRahil Ghanbarnasab Behbahani0Amir Danyaei1Hamed Shoghi2Mohammad Javad Tahmasbi3Ghasem Saki4Niloofar Neisi5Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCellular and Molecular Research Center, Medical Basic Sciencec Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCellular and Molecular Research Center, Medical Basic Sciencec Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranObjective(s): Breast cancer cells developing radioresistance during radiation may result in cancer recurrence and poor survival. One of the main reasons for this problem is the changes in the regulation of genes that have a key role in the epithelial-mesenchymal transition (EMT). Utilizing mesenchymal stem cells can be an effective approach to overcome therapeutic resistance. In this study, we investigated the possibility of combining mesenchymal medium with cancer cell medium in sensitizing breast carcinoma cells to radiation. Materials and Methods: In this experimental study, the cells were irradiated at a dose of 4 Gy alone and in combination with stem cells and cancer cells media. Apoptosis, cell cycle, Western blotting, and real-time PCR assays evaluated the therapeutic effects. Results: We found that the CSCM could decrease the expression of several EMT markers (CD133, CD44, Vimentin, Nanog, Snail, and Twist), resulting in increased cell distribution in the G1 and G2/M phases, apoptosis rate, and protein levels of p-Chk2 and cyclin D1; furthermore, it exhibits synergetic effects with radiation treatment in vitro. Conclusion: These findings show that CSCM inhibits the expansion of breast cancer cells and makes them more susceptible to radiotherapy, offering a unique approach to treating breast cancer by overcoming radioresistance.https://ijbms.mums.ac.ir/article_21785_43be12477e5bd2aecf7b09a6e627ac67.pdfbreast carcinomaemt markersmda-mb-231 cells mesenchymal stem cellradiotherapy |
| spellingShingle | Rahil Ghanbarnasab Behbahani Amir Danyaei Hamed Shoghi Mohammad Javad Tahmasbi Ghasem Saki Niloofar Neisi Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cells Iranian Journal of Basic Medical Sciences breast carcinoma emt markers mda-mb-231 cells mesenchymal stem cell radiotherapy |
| title | Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cells |
| title_full | Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cells |
| title_fullStr | Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cells |
| title_full_unstemmed | Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cells |
| title_short | Irradiation and conditioned media from human umbilical cord stem cells suppress epithelial-mesenchymal transition biomarkers in breast cancer cells |
| title_sort | irradiation and conditioned media from human umbilical cord stem cells suppress epithelial mesenchymal transition biomarkers in breast cancer cells |
| topic | breast carcinoma emt markers mda-mb-231 cells mesenchymal stem cell radiotherapy |
| url | https://ijbms.mums.ac.ir/article_21785_43be12477e5bd2aecf7b09a6e627ac67.pdf |
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