Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid Treatment
Th17 cells are implicated in allergic inflammatory diseases, including allergic rhinitis (AR), though the effect of steroids on Th17 cell-dependent nasal responses is unclear. Herein, we investigated a nasal inflammation model elicited by allergen provocation in mice infused with Th17 cells and its...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/5/674 |
| _version_ | 1797501260416417792 |
|---|---|
| author | Shusaku Ueda Kento Miura Hideki Kawasaki Sawako Ogata Norimasa Yamasaki Shuka Miura Akio Mori Osamu Kaminuma |
| author_facet | Shusaku Ueda Kento Miura Hideki Kawasaki Sawako Ogata Norimasa Yamasaki Shuka Miura Akio Mori Osamu Kaminuma |
| author_sort | Shusaku Ueda |
| collection | DOAJ |
| description | Th17 cells are implicated in allergic inflammatory diseases, including allergic rhinitis (AR), though the effect of steroids on Th17 cell-dependent nasal responses is unclear. Herein, we investigated a nasal inflammation model elicited by allergen provocation in mice infused with Th17 cells and its responsiveness against steroid treatment. We transferred BALB/c mice with Th17 cells, which were differentiated in vitro and showed a specific reaction to ovalbumin (OVA). We challenged the transferred mice by intranasal injection of OVA and to some of them, administered dexamethasone (Dex) subcutaneously in advance. Then, we assessed immediate nasal response (INR), nasal hyperresponsiveness (NHR), and inflammatory cell infiltration into the nasal mucosa. The significant nasal inflammatory responses with massive neutrophil accumulation, INR, and NHR were induced upon allergen challenge. Allergen-induced INR and NHR were significantly suppressed by Dex treatment. This study suggested the effectiveness of steroids on Th17 cell-mediated nasal responses in AR. |
| first_indexed | 2024-03-10T03:15:50Z |
| format | Article |
| id | doaj.art-ae72503b8e2748abbe35dfc044973538 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T03:15:50Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-ae72503b8e2748abbe35dfc0449735382023-11-23T10:13:55ZengMDPI AGBiomolecules2218-273X2022-05-0112567410.3390/biom12050674Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid TreatmentShusaku Ueda0Kento Miura1Hideki Kawasaki2Sawako Ogata3Norimasa Yamasaki4Shuka Miura5Akio Mori6Osamu Kaminuma7Department of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanClinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Kanagawa 252-0392, JapanDepartment of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanTh17 cells are implicated in allergic inflammatory diseases, including allergic rhinitis (AR), though the effect of steroids on Th17 cell-dependent nasal responses is unclear. Herein, we investigated a nasal inflammation model elicited by allergen provocation in mice infused with Th17 cells and its responsiveness against steroid treatment. We transferred BALB/c mice with Th17 cells, which were differentiated in vitro and showed a specific reaction to ovalbumin (OVA). We challenged the transferred mice by intranasal injection of OVA and to some of them, administered dexamethasone (Dex) subcutaneously in advance. Then, we assessed immediate nasal response (INR), nasal hyperresponsiveness (NHR), and inflammatory cell infiltration into the nasal mucosa. The significant nasal inflammatory responses with massive neutrophil accumulation, INR, and NHR were induced upon allergen challenge. Allergen-induced INR and NHR were significantly suppressed by Dex treatment. This study suggested the effectiveness of steroids on Th17 cell-mediated nasal responses in AR.https://www.mdpi.com/2218-273X/12/5/674inflammationnasal hyperresponsivenessTh17 celldexamethasonemouse |
| spellingShingle | Shusaku Ueda Kento Miura Hideki Kawasaki Sawako Ogata Norimasa Yamasaki Shuka Miura Akio Mori Osamu Kaminuma Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid Treatment Biomolecules inflammation nasal hyperresponsiveness Th17 cell dexamethasone mouse |
| title | Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid Treatment |
| title_full | Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid Treatment |
| title_fullStr | Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid Treatment |
| title_full_unstemmed | Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid Treatment |
| title_short | Th17-Dependent Nasal Hyperresponsiveness Is Mitigated by Steroid Treatment |
| title_sort | th17 dependent nasal hyperresponsiveness is mitigated by steroid treatment |
| topic | inflammation nasal hyperresponsiveness Th17 cell dexamethasone mouse |
| url | https://www.mdpi.com/2218-273X/12/5/674 |
| work_keys_str_mv | AT shusakuueda th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment AT kentomiura th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment AT hidekikawasaki th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment AT sawakoogata th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment AT norimasayamasaki th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment AT shukamiura th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment AT akiomori th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment AT osamukaminuma th17dependentnasalhyperresponsivenessismitigatedbysteroidtreatment |