In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureus
Background and Objectives: The antimicrobial combination with synergistic mechanism is recommended to provide broad-spectrum coverage, and prevent the emergence of resistant mutants. In the present study, the synergistic activity of lysostaphin with linezolid, oxacillin and vancomycin, against methi...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-11-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/1285 |
| _version_ | 1798027542107521024 |
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| author | Nagalakshmi Narasimhaswamy Indira Bairy Gautham Shenoy Laxminarayana Bairy |
| author_facet | Nagalakshmi Narasimhaswamy Indira Bairy Gautham Shenoy Laxminarayana Bairy |
| author_sort | Nagalakshmi Narasimhaswamy |
| collection | DOAJ |
| description | Background and Objectives: The antimicrobial combination with synergistic mechanism is recommended to provide broad-spectrum coverage, and prevent the emergence of resistant mutants. In the present study, the synergistic activity of lysostaphin with linezolid, oxacillin and vancomycin, against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates was determined.
Materials and Methods: Seventy-three MRSA isolates collected from clinical specimens were tested, for in vitro synergistic activity of lysostaphin with linezolid, vancomycin and oxacillin, by checkerboard assay.
Results: Lysostaphin showed synergistic activity with linezolid and oxacillin, against all MRSA isolates, tested in the present study. Whereas, only 19.1% of the isolates showed synergistic activity with vancomycin and remaining 80.9% of the MRSA isolates showed additive activity.
Conclusion: Lysostaphin causes rapid lysis of S. aureus. Combination therapies that include linezolid and lysostaphin could be used in life-threatening infections, such as endocarditis to increase the early in vivo activity of the antibiotics, and to prevent the emergence of linezolid resistant mutants. Further, in vivo studies are warranted to confirm our results. |
| first_indexed | 2024-04-11T18:53:26Z |
| format | Article |
| id | doaj.art-ae726785237f4adfb774b9300dbfdaed |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-11T18:53:26Z |
| publishDate | 2017-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-ae726785237f4adfb774b9300dbfdaed2022-12-22T04:08:17ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472017-11-0194In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureusNagalakshmi Narasimhaswamy0Indira Bairy1Gautham Shenoy2Laxminarayana Bairy3Department of Microbiology, Melaka Manipal Medical College (Manipal Campus) Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Microbiology, Melaka Manipal Medical College (Manipal Campus) Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Pharmacology, RAK Medical and Health Sciences University, Ras Al Khaimah, UAEBackground and Objectives: The antimicrobial combination with synergistic mechanism is recommended to provide broad-spectrum coverage, and prevent the emergence of resistant mutants. In the present study, the synergistic activity of lysostaphin with linezolid, oxacillin and vancomycin, against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates was determined. Materials and Methods: Seventy-three MRSA isolates collected from clinical specimens were tested, for in vitro synergistic activity of lysostaphin with linezolid, vancomycin and oxacillin, by checkerboard assay. Results: Lysostaphin showed synergistic activity with linezolid and oxacillin, against all MRSA isolates, tested in the present study. Whereas, only 19.1% of the isolates showed synergistic activity with vancomycin and remaining 80.9% of the MRSA isolates showed additive activity. Conclusion: Lysostaphin causes rapid lysis of S. aureus. Combination therapies that include linezolid and lysostaphin could be used in life-threatening infections, such as endocarditis to increase the early in vivo activity of the antibiotics, and to prevent the emergence of linezolid resistant mutants. Further, in vivo studies are warranted to confirm our results.https://ijm.tums.ac.ir/index.php/ijm/article/view/1285LysostaphinMethicillin resistant Staphylococcus aureus (MRSA)Synergistic activity |
| spellingShingle | Nagalakshmi Narasimhaswamy Indira Bairy Gautham Shenoy Laxminarayana Bairy In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureus Iranian Journal of Microbiology Lysostaphin Methicillin resistant Staphylococcus aureus (MRSA) Synergistic activity |
| title | In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureus |
| title_full | In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureus |
| title_fullStr | In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureus |
| title_short | In vitro activity of recombinant lysostaphin in combination with linezolid, vancomycin and oxacillin against methicillin-resistant Staphylococcus aureus |
| title_sort | in vitro activity of recombinant lysostaphin in combination with linezolid vancomycin and oxacillin against methicillin resistant staphylococcus aureus |
| topic | Lysostaphin Methicillin resistant Staphylococcus aureus (MRSA) Synergistic activity |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/1285 |
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