Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its Endolysin
<i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a phytopathogen that causes canker in kiwifruit. Few conventional control methods are effective against this bacterium. Therefore, alternative approaches, such as phage therapy are warranted. In this study, a lytic ba...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/4/631 |
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| author | Peien Ni Lei Wang Bohan Deng Songtao Jiu Chao Ma Caixi Zhang Adelaide Almeida Dapeng Wang Wenping Xu Shiping Wang |
| author_facet | Peien Ni Lei Wang Bohan Deng Songtao Jiu Chao Ma Caixi Zhang Adelaide Almeida Dapeng Wang Wenping Xu Shiping Wang |
| author_sort | Peien Ni |
| collection | DOAJ |
| description | <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a phytopathogen that causes canker in kiwifruit. Few conventional control methods are effective against this bacterium. Therefore, alternative approaches, such as phage therapy are warranted. In this study, a lytic bacteriophage (PN09) of Psa was isolated from surface water collected from a river in Hangzhou, China in 2019. Morphologically, PN09 was classified into the <i>Myoviridae</i> family, and could lyse all 29 Psa biovar 3 strains. The optimal temperature and pH ranges for PN09 activity were determined as 25 to 35 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mrow></mrow><mo>∘</mo></msup></semantics></math></inline-formula>C and 6.0 to 9.0, respectively. The complete genome of PN09 was found to be composed of a linear 99,229 bp double-stranded DNA genome with a GC content of 48.16%. The PN09 endolysin (LysPN09) was expressed in vitro and characterized. LysPN09 was predicted to belong to the Muraidase superfamily domain and showed lytic activity against the outer-membrane-permeabilized Psa strains. The lytic activity of LysPN09 was optimal over temperature and pH ranges of 25 to 40 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mrow></mrow><mo>∘</mo></msup></semantics></math></inline-formula>C and 6.0 to 8.0, respectively. When recombinant endolysin LysPN09 was combined with EDTA, Psa strains were effectively damaged. All these characteristics demonstrate that the phage PN09 and its endolysin, LysPN09, are potential candidates for biocontrol of Psa in the kiwifruit industry. |
| first_indexed | 2024-03-10T12:33:05Z |
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| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T12:33:05Z |
| publishDate | 2021-04-01 |
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| spelling | doaj.art-ae732dc07c9649f4b74239cd68e368bb2023-11-21T14:31:34ZengMDPI AGViruses1999-49152021-04-0113463110.3390/v13040631Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its EndolysinPeien Ni0Lei Wang1Bohan Deng2Songtao Jiu3Chao Ma4Caixi Zhang5Adelaide Almeida6Dapeng Wang7Wenping Xu8Shiping Wang9School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China<i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a phytopathogen that causes canker in kiwifruit. Few conventional control methods are effective against this bacterium. Therefore, alternative approaches, such as phage therapy are warranted. In this study, a lytic bacteriophage (PN09) of Psa was isolated from surface water collected from a river in Hangzhou, China in 2019. Morphologically, PN09 was classified into the <i>Myoviridae</i> family, and could lyse all 29 Psa biovar 3 strains. The optimal temperature and pH ranges for PN09 activity were determined as 25 to 35 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mrow></mrow><mo>∘</mo></msup></semantics></math></inline-formula>C and 6.0 to 9.0, respectively. The complete genome of PN09 was found to be composed of a linear 99,229 bp double-stranded DNA genome with a GC content of 48.16%. The PN09 endolysin (LysPN09) was expressed in vitro and characterized. LysPN09 was predicted to belong to the Muraidase superfamily domain and showed lytic activity against the outer-membrane-permeabilized Psa strains. The lytic activity of LysPN09 was optimal over temperature and pH ranges of 25 to 40 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mrow></mrow><mo>∘</mo></msup></semantics></math></inline-formula>C and 6.0 to 8.0, respectively. When recombinant endolysin LysPN09 was combined with EDTA, Psa strains were effectively damaged. All these characteristics demonstrate that the phage PN09 and its endolysin, LysPN09, are potential candidates for biocontrol of Psa in the kiwifruit industry.https://www.mdpi.com/1999-4915/13/4/631<i>Pseudomonas syringae</i> pv. <i>actinidiae</i>kiwifruitlytic bacteriophageendolysinphage therapy |
| spellingShingle | Peien Ni Lei Wang Bohan Deng Songtao Jiu Chao Ma Caixi Zhang Adelaide Almeida Dapeng Wang Wenping Xu Shiping Wang Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its Endolysin Viruses <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> kiwifruit lytic bacteriophage endolysin phage therapy |
| title | Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its Endolysin |
| title_full | Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its Endolysin |
| title_fullStr | Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its Endolysin |
| title_full_unstemmed | Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its Endolysin |
| title_short | Characterization of a Lytic Bacteriophage against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> and Its Endolysin |
| title_sort | characterization of a lytic bacteriophage against i pseudomonas syringae i pv i actinidiae i and its endolysin |
| topic | <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> kiwifruit lytic bacteriophage endolysin phage therapy |
| url | https://www.mdpi.com/1999-4915/13/4/631 |
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