Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001
Abstract Background Co-production of polyhydroxyalkanoate (PHA) and amino acids makes bacteria effective microbial cell factories by secreting amino acids outside while accumulating PHA granules inside. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is one of the PHAs with biocompatibility and...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-018-0942-7 |
| _version_ | 1818900062241030144 |
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| author | Wenjian Ma Jianli Wang Ye Li Lianghong Yin Xiaoyuan Wang |
| author_facet | Wenjian Ma Jianli Wang Ye Li Lianghong Yin Xiaoyuan Wang |
| author_sort | Wenjian Ma |
| collection | DOAJ |
| description | Abstract Background Co-production of polyhydroxyalkanoate (PHA) and amino acids makes bacteria effective microbial cell factories by secreting amino acids outside while accumulating PHA granules inside. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is one of the PHAs with biocompatibility and fine mechanical properties, but its production is limited by the low level of intracellular propionyl-CoA. Results l-Isoleucine producing Corynebacterium glutamicum strain WM001 were analyzed by genome and transcriptome sequencing. The results showed that the most over-expressed genes in WM001 are relevant not only to l-isoleucine production but also to propionyl-CoA accumulation. Compared to the wild-type C. glutamicum ATCC13869, the transcriptional levels of the genes prpC2, prpD2, and prpB2, which are key genes relevant to propionyl-CoA accumulation, increased 26.7, 25.8, and 28.4-folds in WM001, respectively; and the intracellular level of propionyl-CoA increased 16.9-fold in WM001. When the gene cluster phaCAB for PHA biosynthesis was introduced into WM001, the recombinant strain WM001/pDXW-8-phaCAB produced 15.0 g/L PHBV with high percentage of 3-hydroxyvalerate as well as 29.8 g/L l-isoleucine after fed-batch fermentation. The maximum 3-hydroxyvalerate fraction in PHBV produced by WM001/pDXW-8-phaCAB using glucose as the sole carbon source could reach 72.5%, which is the highest reported so far. Conclusions Genome and transcriptome analysis showed that C. glutamicum WM001 has potential to accumulate l-isoleucine and propionyl-CoA pool. This was experimentally confirmed by introducing the phaCAB gene cluster into WM001. The recombinant strain WM001/pDXW-8-phaCAB produced high levels of PHBV with high 3-hydroxyvalerate fraction as well as l-isoleucine. Because of its high level of intracellular propionyl-CoA pool, WM001 might be used for producing other propionyl-CoA derivatives. |
| first_indexed | 2024-12-19T19:57:53Z |
| format | Article |
| id | doaj.art-ae73a43bea25402ba4271bd40ced925c |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-19T19:57:53Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
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| series | Microbial Cell Factories |
| spelling | doaj.art-ae73a43bea25402ba4271bd40ced925c2022-12-21T20:07:45ZengBMCMicrobial Cell Factories1475-28592018-06-0117111210.1186/s12934-018-0942-7Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001Wenjian Ma0Jianli Wang1Ye Li2Lianghong Yin3Xiaoyuan Wang4State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityInternational Joint Laboratory on Food Safety, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityAbstract Background Co-production of polyhydroxyalkanoate (PHA) and amino acids makes bacteria effective microbial cell factories by secreting amino acids outside while accumulating PHA granules inside. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is one of the PHAs with biocompatibility and fine mechanical properties, but its production is limited by the low level of intracellular propionyl-CoA. Results l-Isoleucine producing Corynebacterium glutamicum strain WM001 were analyzed by genome and transcriptome sequencing. The results showed that the most over-expressed genes in WM001 are relevant not only to l-isoleucine production but also to propionyl-CoA accumulation. Compared to the wild-type C. glutamicum ATCC13869, the transcriptional levels of the genes prpC2, prpD2, and prpB2, which are key genes relevant to propionyl-CoA accumulation, increased 26.7, 25.8, and 28.4-folds in WM001, respectively; and the intracellular level of propionyl-CoA increased 16.9-fold in WM001. When the gene cluster phaCAB for PHA biosynthesis was introduced into WM001, the recombinant strain WM001/pDXW-8-phaCAB produced 15.0 g/L PHBV with high percentage of 3-hydroxyvalerate as well as 29.8 g/L l-isoleucine after fed-batch fermentation. The maximum 3-hydroxyvalerate fraction in PHBV produced by WM001/pDXW-8-phaCAB using glucose as the sole carbon source could reach 72.5%, which is the highest reported so far. Conclusions Genome and transcriptome analysis showed that C. glutamicum WM001 has potential to accumulate l-isoleucine and propionyl-CoA pool. This was experimentally confirmed by introducing the phaCAB gene cluster into WM001. The recombinant strain WM001/pDXW-8-phaCAB produced high levels of PHBV with high 3-hydroxyvalerate fraction as well as l-isoleucine. Because of its high level of intracellular propionyl-CoA pool, WM001 might be used for producing other propionyl-CoA derivatives.http://link.springer.com/article/10.1186/s12934-018-0942-7Corynebacterium glutamicuml-IsoleucinePHBV3HV fractionPropionyl-CoA |
| spellingShingle | Wenjian Ma Jianli Wang Ye Li Lianghong Yin Xiaoyuan Wang Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001 Microbial Cell Factories Corynebacterium glutamicum l-Isoleucine PHBV 3HV fraction Propionyl-CoA |
| title | Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001 |
| title_full | Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001 |
| title_fullStr | Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001 |
| title_full_unstemmed | Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001 |
| title_short | Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001 |
| title_sort | poly 3 hydroxybutyrate co 3 hydroxyvalerate co produced with l isoleucine in corynebacterium glutamicum wm001 |
| topic | Corynebacterium glutamicum l-Isoleucine PHBV 3HV fraction Propionyl-CoA |
| url | http://link.springer.com/article/10.1186/s12934-018-0942-7 |
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