Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional study
Abstract Background and Aims Odontogenic cysts and tumors often form hard and soft structures that resemble odontogenesis. It is well known that amyloid is produced in Pindborg tumors; however, it is still debatable whether it is also formed in other odontogenic tumors and cysts. This study aimed to...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Health Science Reports |
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| Online Access: | https://doi.org/10.1002/hsr2.1061 |
| _version_ | 1797737724715728896 |
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| author | Haider H. Al‐Qazzaz Bashar H. Abdullah Omar S. Museedi |
| author_facet | Haider H. Al‐Qazzaz Bashar H. Abdullah Omar S. Museedi |
| author_sort | Haider H. Al‐Qazzaz |
| collection | DOAJ |
| description | Abstract Background and Aims Odontogenic cysts and tumors often form hard and soft structures that resemble odontogenesis. It is well known that amyloid is produced in Pindborg tumors; however, it is still debatable whether it is also formed in other odontogenic tumors and cysts. This study aimed to detect the presence of amyloid in different odontogenic cysts and tumors in correlation to matrix proteins secreted during enamel formation; namely amelogenin and odontogenic ameloblast‐associated protein. Methods This study included formalin fixed paraffin embedded tissue blocks of 106 different types of odontogenic cysts and tumors. Congo red and thioflavin T were performed to confirm the presence of amyloid; immunohistochemistry was used to detect amelogenin and odontogenic ameloblast‐associated protein. Results Amyloid was detected in pindborg tumors (conventional), adenomatoid odontogenic tumors, odontogenic fibroma (Amyloid variant), follicular solid and unicystic ameloblastomas, radicular cysts, dentigerous cysts, dentinogenic ghost cell odontogenic tumor, ameloblastic fibroma, calcifying odontogenic cyst, and primordial Odontogenic tumor. Amelogenin was detected in 95.3% of the cases, while odontogenic ameloblast‐associated protein was detected in 93.4% of the cases. The association between odontogenic ameloblast‐associated protein and amyloid was highly significant at p < 0.01. However, there was no significant relationship between amelogenin and amyloid p > 0.05. Conclusion Although pindborg tumor is the bonafide example of amyloid deposition in odontogenic tumors, this study concluded that amyloid may be deposited in traces to massive amounts in various odontogenic cysts and tumors, and it is significantly linked to odontogenic ameloblast‐associated protein but not amelogenin matrix protein, since all amyloid cases were odontogenic ameloblast associated protein positive. |
| first_indexed | 2024-03-12T13:33:34Z |
| format | Article |
| id | doaj.art-ae748ccff05c496a90e55bc164225620 |
| institution | Directory Open Access Journal |
| issn | 2398-8835 |
| language | English |
| last_indexed | 2024-03-12T13:33:34Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Health Science Reports |
| spelling | doaj.art-ae748ccff05c496a90e55bc1642256202023-08-24T06:32:46ZengWileyHealth Science Reports2398-88352023-01-0161n/an/a10.1002/hsr2.1061Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional studyHaider H. Al‐Qazzaz0Bashar H. Abdullah1Omar S. Museedi2Department of Pathology Al Yarmouk Teaching Hospital/Teaching Laboratories Baghdad IraqDepartment of Oral Diagnosis, College of Dentistry University of Baghdad Baghdad IraqDepartment of Oral Diagnosis, College of Dentistry University of Baghdad Baghdad IraqAbstract Background and Aims Odontogenic cysts and tumors often form hard and soft structures that resemble odontogenesis. It is well known that amyloid is produced in Pindborg tumors; however, it is still debatable whether it is also formed in other odontogenic tumors and cysts. This study aimed to detect the presence of amyloid in different odontogenic cysts and tumors in correlation to matrix proteins secreted during enamel formation; namely amelogenin and odontogenic ameloblast‐associated protein. Methods This study included formalin fixed paraffin embedded tissue blocks of 106 different types of odontogenic cysts and tumors. Congo red and thioflavin T were performed to confirm the presence of amyloid; immunohistochemistry was used to detect amelogenin and odontogenic ameloblast‐associated protein. Results Amyloid was detected in pindborg tumors (conventional), adenomatoid odontogenic tumors, odontogenic fibroma (Amyloid variant), follicular solid and unicystic ameloblastomas, radicular cysts, dentigerous cysts, dentinogenic ghost cell odontogenic tumor, ameloblastic fibroma, calcifying odontogenic cyst, and primordial Odontogenic tumor. Amelogenin was detected in 95.3% of the cases, while odontogenic ameloblast‐associated protein was detected in 93.4% of the cases. The association between odontogenic ameloblast‐associated protein and amyloid was highly significant at p < 0.01. However, there was no significant relationship between amelogenin and amyloid p > 0.05. Conclusion Although pindborg tumor is the bonafide example of amyloid deposition in odontogenic tumors, this study concluded that amyloid may be deposited in traces to massive amounts in various odontogenic cysts and tumors, and it is significantly linked to odontogenic ameloblast‐associated protein but not amelogenin matrix protein, since all amyloid cases were odontogenic ameloblast associated protein positive.https://doi.org/10.1002/hsr2.1061amyloidCongo redodontogenic cystsodontogenic tumorsthioflavin T |
| spellingShingle | Haider H. Al‐Qazzaz Bashar H. Abdullah Omar S. Museedi Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional study Health Science Reports amyloid Congo red odontogenic cysts odontogenic tumors thioflavin T |
| title | Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional study |
| title_full | Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional study |
| title_fullStr | Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional study |
| title_full_unstemmed | Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional study |
| title_short | Correlation of amyloid and ameloblast‐associated proteins to odontogenic cysts and tumors: A cross‐sectional study |
| title_sort | correlation of amyloid and ameloblast associated proteins to odontogenic cysts and tumors a cross sectional study |
| topic | amyloid Congo red odontogenic cysts odontogenic tumors thioflavin T |
| url | https://doi.org/10.1002/hsr2.1061 |
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