Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells
Micrometer-sized silicon chips have been demonstrated to be cell-internalizable, offering the possibility of introducing in cells even smaller nanoelements for intracellular applications. On the other hand, silicon nanowires on extracellular devices have been widely studied as biosensors or drug del...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/5/893 |
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| author | Sara Durán Marta Duch Rodrigo Gómez-Martínez Marta Fernández-Regúlez Juan Pablo Agusil Manuel Reina Claudia Müller Álvaro San Paulo Jaume Esteve Susana Castel José A. Plaza |
| author_facet | Sara Durán Marta Duch Rodrigo Gómez-Martínez Marta Fernández-Regúlez Juan Pablo Agusil Manuel Reina Claudia Müller Álvaro San Paulo Jaume Esteve Susana Castel José A. Plaza |
| author_sort | Sara Durán |
| collection | DOAJ |
| description | Micrometer-sized silicon chips have been demonstrated to be cell-internalizable, offering the possibility of introducing in cells even smaller nanoelements for intracellular applications. On the other hand, silicon nanowires on extracellular devices have been widely studied as biosensors or drug delivery systems. Here, we propose the integration of silicon nanowires on cell-internalizable chips in order to combine the functional features of both approaches for advanced intracellular applications. As an initial fundamental study, the cellular uptake in HeLa cells of silicon 3 µm × 3 µm nanowire-based chips with two different morphologies was investigated, and the results were compared with those of non-nanostructured silicon chips. Chip internalization without affecting cell viability was achieved in all cases; however, important cell behavior differences were observed. In particular, the first stage of cell internalization was favored by silicon nanowire interfaces with respect to bulk silicon. In addition, chips were found inside membrane vesicles, and some nanowires seemed to penetrate the cytosol, which opens the door to the development of silicon nanowire chips as future intracellular sensors and drug delivery systems. |
| first_indexed | 2024-03-10T19:58:39Z |
| format | Article |
| id | doaj.art-ae7a828bee7343be9daee3560b239716 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T19:58:39Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-ae7a828bee7343be9daee3560b2397162023-11-19T23:44:47ZengMDPI AGNanomaterials2079-49912020-05-0110589310.3390/nano10050893Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa CellsSara Durán0Marta Duch1Rodrigo Gómez-Martínez2Marta Fernández-Regúlez3Juan Pablo Agusil4Manuel Reina5Claudia Müller6Álvaro San Paulo7Jaume Esteve8Susana Castel9José A. Plaza10Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola, 08193 Barcelona, SpainInstituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola, 08193 Barcelona, SpainInstituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola, 08193 Barcelona, SpainInstituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola, 08193 Barcelona, SpainInstituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola, 08193 Barcelona, SpainDepartamento de Biología Celular, Fisiología e Inmunología, Facultad de Biología, Universitat de Barcelona, 08028 Barcelona, SpainDepartamento de Biología Celular, Fisiología e Inmunología, Facultad de Biología, Universitat de Barcelona, 08028 Barcelona, SpainInstituto de Microelectrónica de Madrid, IMM-CNM (CSIC), Isaac Newton 8, Tres Cantos, 28760 Madrid, SpainInstituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola, 08193 Barcelona, SpainDepartamento de Biología Celular, Fisiología e Inmunología, Facultad de Biología, Universitat de Barcelona, 08028 Barcelona, SpainInstituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Cerdanyola, 08193 Barcelona, SpainMicrometer-sized silicon chips have been demonstrated to be cell-internalizable, offering the possibility of introducing in cells even smaller nanoelements for intracellular applications. On the other hand, silicon nanowires on extracellular devices have been widely studied as biosensors or drug delivery systems. Here, we propose the integration of silicon nanowires on cell-internalizable chips in order to combine the functional features of both approaches for advanced intracellular applications. As an initial fundamental study, the cellular uptake in HeLa cells of silicon 3 µm × 3 µm nanowire-based chips with two different morphologies was investigated, and the results were compared with those of non-nanostructured silicon chips. Chip internalization without affecting cell viability was achieved in all cases; however, important cell behavior differences were observed. In particular, the first stage of cell internalization was favored by silicon nanowire interfaces with respect to bulk silicon. In addition, chips were found inside membrane vesicles, and some nanowires seemed to penetrate the cytosol, which opens the door to the development of silicon nanowire chips as future intracellular sensors and drug delivery systems.https://www.mdpi.com/2079-4991/10/5/893siliconnanowirescellsbiomimeticsmicrotechnologymicroparticles |
| spellingShingle | Sara Durán Marta Duch Rodrigo Gómez-Martínez Marta Fernández-Regúlez Juan Pablo Agusil Manuel Reina Claudia Müller Álvaro San Paulo Jaume Esteve Susana Castel José A. Plaza Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells Nanomaterials silicon nanowires cells biomimetics microtechnology microparticles |
| title | Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells |
| title_full | Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells |
| title_fullStr | Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells |
| title_full_unstemmed | Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells |
| title_short | Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells |
| title_sort | internalization and viability studies of suspended nanowire silicon chips in hela cells |
| topic | silicon nanowires cells biomimetics microtechnology microparticles |
| url | https://www.mdpi.com/2079-4991/10/5/893 |
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