The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar Rats
<strong>Introduction:</strong> Deep heated oils produce toxins that pose significant risk to the human health. The present study aims to investigate the simultaneous effects of aerobic training and octopamine consumption on the mitophagy of the brown adipose tissue after the induction of...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-09-01
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| Series: | Journal of Fasting and Health |
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| Online Access: | http://jnfh.mums.ac.ir/article_15663_fbaad3ee081272fe67fde89548686e2f.pdf |
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| author | Sadegh Abdollahi Khalid Mohamadzadeh Salamat Kamal Azizbeigi Zaher Etemad |
| author_facet | Sadegh Abdollahi Khalid Mohamadzadeh Salamat Kamal Azizbeigi Zaher Etemad |
| author_sort | Sadegh Abdollahi |
| collection | DOAJ |
| description | <strong>Introduction:</strong> Deep heated oils produce toxins that pose significant risk to the human health. The present study aims to investigate the simultaneous effects of aerobic training and octopamine consumption on the mitophagy of the brown adipose tissue after the induction of intoxication by deeply heated oil in male Wistar rats. <strong>Methods: </strong>This study was conducted on 40 male Wistar rats after four weeks of feeding with heated oil. The animals were randomly assigned to the groups of control-intoxication (CI; n=8), exercise training-intoxication (ETI; n=8), supplement-intoxication (SI; n=8), supplement-exercise-intoxication (SEI; n=8), and healthy control (HC; n=8).Exercise training was performed for four weeks at the intensity of 50-65% VO<sub>2</sub>max for 20 minutes per session. Octopamine was used as the supplement for four weeks. In addition, intraperitoneal injection was carried out with the dose of 81 µmol/kg five days per week in the CI, SI, and SEI groups. Real-time polymerase chain reaction (RT-PCR) was applied to evaluate the expression of the PTEN-induced kinase 1 (<em>PINK1</em>) and lysosome-associated membrane protein 2 (<em>LAMP2A</em>) genes. <strong>Results: </strong>No significant difference was observed in the <em>PINK1</em> gene expression between the CI, ETI, and SEI groups (P˃0.05), while a significant difference was observed between the CI, ETI, and SEI groups with the HC in this regard (P≤0.05). The <em>LAMP2</em> gene expression significantly was higher in the HC group compared to the other groups (P≤0.05). In addition, the <em>LAMP2</em> gene expression in the SEI group was significantly higher than the HC group (P=0.001). <strong>Conclusion: </strong>According to the results, the combination of aerobic training and octopamine supplementation led to mitophagy induction through intoxication with heated oils by increasing the expression of the <em>LAMP2A</em> gene and induction of chaperones by the activation of the chaperones with binding to the LAMP2 receptor protein in the lysosome. |
| first_indexed | 2024-12-13T15:59:47Z |
| format | Article |
| id | doaj.art-ae7cb45200f444b1880553bd83343106 |
| institution | Directory Open Access Journal |
| issn | 2345-2587 2345-2587 |
| language | English |
| last_indexed | 2024-12-13T15:59:47Z |
| publishDate | 2020-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasting and Health |
| spelling | doaj.art-ae7cb45200f444b1880553bd833431062022-12-21T23:39:13ZengMashhad University of Medical SciencesJournal of Fasting and Health2345-25872345-25872020-09-018315115810.22038/jnfh.2020.47446.125615663The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar RatsSadegh Abdollahi0Khalid Mohamadzadeh Salamat1Kamal Azizbeigi2Zaher Etemad3PhD Student, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.Assistant Professor, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, IranAssociate Professor, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.Assistant Professor, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.<strong>Introduction:</strong> Deep heated oils produce toxins that pose significant risk to the human health. The present study aims to investigate the simultaneous effects of aerobic training and octopamine consumption on the mitophagy of the brown adipose tissue after the induction of intoxication by deeply heated oil in male Wistar rats. <strong>Methods: </strong>This study was conducted on 40 male Wistar rats after four weeks of feeding with heated oil. The animals were randomly assigned to the groups of control-intoxication (CI; n=8), exercise training-intoxication (ETI; n=8), supplement-intoxication (SI; n=8), supplement-exercise-intoxication (SEI; n=8), and healthy control (HC; n=8).Exercise training was performed for four weeks at the intensity of 50-65% VO<sub>2</sub>max for 20 minutes per session. Octopamine was used as the supplement for four weeks. In addition, intraperitoneal injection was carried out with the dose of 81 µmol/kg five days per week in the CI, SI, and SEI groups. Real-time polymerase chain reaction (RT-PCR) was applied to evaluate the expression of the PTEN-induced kinase 1 (<em>PINK1</em>) and lysosome-associated membrane protein 2 (<em>LAMP2A</em>) genes. <strong>Results: </strong>No significant difference was observed in the <em>PINK1</em> gene expression between the CI, ETI, and SEI groups (P˃0.05), while a significant difference was observed between the CI, ETI, and SEI groups with the HC in this regard (P≤0.05). The <em>LAMP2</em> gene expression significantly was higher in the HC group compared to the other groups (P≤0.05). In addition, the <em>LAMP2</em> gene expression in the SEI group was significantly higher than the HC group (P=0.001). <strong>Conclusion: </strong>According to the results, the combination of aerobic training and octopamine supplementation led to mitophagy induction through intoxication with heated oils by increasing the expression of the <em>LAMP2A</em> gene and induction of chaperones by the activation of the chaperones with binding to the LAMP2 receptor protein in the lysosome.http://jnfh.mums.ac.ir/article_15663_fbaad3ee081272fe67fde89548686e2f.pdfoctopaminelamp2apinkaerobic training |
| spellingShingle | Sadegh Abdollahi Khalid Mohamadzadeh Salamat Kamal Azizbeigi Zaher Etemad The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar Rats Journal of Fasting and Health octopamine lamp2a pink aerobic training |
| title | The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar Rats |
| title_full | The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar Rats |
| title_fullStr | The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar Rats |
| title_full_unstemmed | The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar Rats |
| title_short | The Simultaneous Effects of Aerobic Training and Octopamine on the Mitophagy of the Brown Adipose Tissue after the Induction of Intoxication by Deeply Heated Oil in Male Wistar Rats |
| title_sort | simultaneous effects of aerobic training and octopamine on the mitophagy of the brown adipose tissue after the induction of intoxication by deeply heated oil in male wistar rats |
| topic | octopamine lamp2a pink aerobic training |
| url | http://jnfh.mums.ac.ir/article_15663_fbaad3ee081272fe67fde89548686e2f.pdf |
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