Bone plasticity in response to exercise is sex-dependent in rats.
To characterize the potential sexual dimorphism of bone in response to exercise.Young male and female Wistar rats were either submitted to 12 weeks of exercise or remained sedentary. The training load was adjusted at the mid-trial (week 6) by the maximal speed test. A mechanical test was performed t...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3669412?pdf=render |
| _version_ | 1819070557286563840 |
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| author | Wagner S Vicente Luciene M dos Reis Rafael G Graciolli Fabiana G Graciolli Wagner V Dominguez Charles C Wang Tatiana L Fonseca Ana P Velosa Hamilton Roschel Walcy R Teodoro Bruno Gualano Vanda Jorgetti |
| author_facet | Wagner S Vicente Luciene M dos Reis Rafael G Graciolli Fabiana G Graciolli Wagner V Dominguez Charles C Wang Tatiana L Fonseca Ana P Velosa Hamilton Roschel Walcy R Teodoro Bruno Gualano Vanda Jorgetti |
| author_sort | Wagner S Vicente |
| collection | DOAJ |
| description | To characterize the potential sexual dimorphism of bone in response to exercise.Young male and female Wistar rats were either submitted to 12 weeks of exercise or remained sedentary. The training load was adjusted at the mid-trial (week 6) by the maximal speed test. A mechanical test was performed to measure the maximal force, resilience, stiffness, and fracture load. The bone structure, formation, and resorption were obtained by histomorphometric analyses. Type I collagen (COL I) mRNA expression and tartrate-resistant acid phosphatase (TRAP) mRNA expression were evaluated by quantitative real-time PCR (qPCR).The male and female trained rats significantly improved their maximum speed during the maximal exercise test (main effect of training; p<0.0001). The male rats were significantly heavier than the females, irrespective of training (main effect of sex; p<0.0001). Similarly, both the weight and length of the femur were greater for the male rats when compared with the females (main effect of sex; p<0.0001 and p<0.0001, respectively). The trabecular volume was positively affected by exercise in male and female rats (main effect of training; p = 0.001), whereas the trabecular thickness, resilience, mineral apposition rate, and bone formation rate increased only in the trained males (within-sex comparison; p<0.05 for all parameters), demonstrating the sexual dimorphism in response to exercise. Accordingly, the number of osteocytes increased significantly only in the trained males (within-sex comparison; p<0.05). Pearson's correlation analyses revealed that the COL I mRNA expression and TRAP mRNA expression were positively and negatively, respectively, related to the parameters of bone remodeling obtained from the histomorphometric analysis (r = 0.59 to 0.85; p<0.05).Exercise yielded differential adaptations with respect to bone structure, biomechanical proprieties, and molecular signaling in male and female rats. |
| first_indexed | 2024-12-21T17:07:50Z |
| format | Article |
| id | doaj.art-ae7dcfdfaa4e432ca5314175c4c46d59 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T17:07:50Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ae7dcfdfaa4e432ca5314175c4c46d592022-12-21T18:56:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6472510.1371/journal.pone.0064725Bone plasticity in response to exercise is sex-dependent in rats.Wagner S VicenteLuciene M dos ReisRafael G GraciolliFabiana G GraciolliWagner V DominguezCharles C WangTatiana L FonsecaAna P VelosaHamilton RoschelWalcy R TeodoroBruno GualanoVanda JorgettiTo characterize the potential sexual dimorphism of bone in response to exercise.Young male and female Wistar rats were either submitted to 12 weeks of exercise or remained sedentary. The training load was adjusted at the mid-trial (week 6) by the maximal speed test. A mechanical test was performed to measure the maximal force, resilience, stiffness, and fracture load. The bone structure, formation, and resorption were obtained by histomorphometric analyses. Type I collagen (COL I) mRNA expression and tartrate-resistant acid phosphatase (TRAP) mRNA expression were evaluated by quantitative real-time PCR (qPCR).The male and female trained rats significantly improved their maximum speed during the maximal exercise test (main effect of training; p<0.0001). The male rats were significantly heavier than the females, irrespective of training (main effect of sex; p<0.0001). Similarly, both the weight and length of the femur were greater for the male rats when compared with the females (main effect of sex; p<0.0001 and p<0.0001, respectively). The trabecular volume was positively affected by exercise in male and female rats (main effect of training; p = 0.001), whereas the trabecular thickness, resilience, mineral apposition rate, and bone formation rate increased only in the trained males (within-sex comparison; p<0.05 for all parameters), demonstrating the sexual dimorphism in response to exercise. Accordingly, the number of osteocytes increased significantly only in the trained males (within-sex comparison; p<0.05). Pearson's correlation analyses revealed that the COL I mRNA expression and TRAP mRNA expression were positively and negatively, respectively, related to the parameters of bone remodeling obtained from the histomorphometric analysis (r = 0.59 to 0.85; p<0.05).Exercise yielded differential adaptations with respect to bone structure, biomechanical proprieties, and molecular signaling in male and female rats.http://europepmc.org/articles/PMC3669412?pdf=render |
| spellingShingle | Wagner S Vicente Luciene M dos Reis Rafael G Graciolli Fabiana G Graciolli Wagner V Dominguez Charles C Wang Tatiana L Fonseca Ana P Velosa Hamilton Roschel Walcy R Teodoro Bruno Gualano Vanda Jorgetti Bone plasticity in response to exercise is sex-dependent in rats. PLoS ONE |
| title | Bone plasticity in response to exercise is sex-dependent in rats. |
| title_full | Bone plasticity in response to exercise is sex-dependent in rats. |
| title_fullStr | Bone plasticity in response to exercise is sex-dependent in rats. |
| title_full_unstemmed | Bone plasticity in response to exercise is sex-dependent in rats. |
| title_short | Bone plasticity in response to exercise is sex-dependent in rats. |
| title_sort | bone plasticity in response to exercise is sex dependent in rats |
| url | http://europepmc.org/articles/PMC3669412?pdf=render |
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