Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild Boars
<i>Actinobacillus pleuropneumoniae</i> is a major pathogen of swine, which can cause severe pleuropneumonia in pigs, but sometimes the disease can be generalized. Diseases caused by <i>A. pleuropneumoniae</i> are frequent all over the world, resulting in high losses among dom...
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MDPI AG
2022-04-01
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| Series: | Pathogens |
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| author | Rita Sárközi László Makrai László Fodor |
| author_facet | Rita Sárközi László Makrai László Fodor |
| author_sort | Rita Sárközi |
| collection | DOAJ |
| description | <i>Actinobacillus pleuropneumoniae</i> is a major pathogen of swine, which can cause severe pleuropneumonia in pigs, but sometimes the disease can be generalized. Diseases caused by <i>A. pleuropneumoniae</i> are frequent all over the world, resulting in high losses among domestic pigs. However, our knowledge on the occurrence of <i>A. pleuropneumoniae</i> in wild boars and feral pigs is limited. We aimed to examine the carriage of <i>A. pleuropneumoniae</i> by hunted wild boars. The presence of <i>A. pleuropneumoniae</i> was examined in tonsils of 68 hunted wild boars collected at a game processing unit. An in-house designed species-specific PCR test was used to detect the gene of Apx IV toxin, and the samples were inoculated on a modified selective agar. <i>A. pleuropneumoniae</i> was detected in 10 animals (14.7%) by PCR and one <i>A. pleuropneumoniae</i> serotype 12 strain was isolated. The antibiotic resistance pattern of the strain resembled field strains that were isolated from farmed pigs in Hungary. This is the first case for the detection of <i>A. pleuropneumoniae</i> not only using PCR or ELISA, but also its isolation, identification, and serotyping. |
| first_indexed | 2024-03-10T03:12:38Z |
| format | Article |
| id | doaj.art-ae80174cafd44ff6a584cd7d2d863a2a |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T03:12:38Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-ae80174cafd44ff6a584cd7d2d863a2a2023-11-23T12:31:41ZengMDPI AGPathogens2076-08172022-04-0111550510.3390/pathogens11050505Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild BoarsRita Sárközi0László Makrai1László Fodor2Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, H-1581 Budapest, HungaryDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, H-1581 Budapest, HungaryDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, H-1581 Budapest, Hungary<i>Actinobacillus pleuropneumoniae</i> is a major pathogen of swine, which can cause severe pleuropneumonia in pigs, but sometimes the disease can be generalized. Diseases caused by <i>A. pleuropneumoniae</i> are frequent all over the world, resulting in high losses among domestic pigs. However, our knowledge on the occurrence of <i>A. pleuropneumoniae</i> in wild boars and feral pigs is limited. We aimed to examine the carriage of <i>A. pleuropneumoniae</i> by hunted wild boars. The presence of <i>A. pleuropneumoniae</i> was examined in tonsils of 68 hunted wild boars collected at a game processing unit. An in-house designed species-specific PCR test was used to detect the gene of Apx IV toxin, and the samples were inoculated on a modified selective agar. <i>A. pleuropneumoniae</i> was detected in 10 animals (14.7%) by PCR and one <i>A. pleuropneumoniae</i> serotype 12 strain was isolated. The antibiotic resistance pattern of the strain resembled field strains that were isolated from farmed pigs in Hungary. This is the first case for the detection of <i>A. pleuropneumoniae</i> not only using PCR or ELISA, but also its isolation, identification, and serotyping.https://www.mdpi.com/2076-0817/11/5/505<i>Actinobacillus pleuropneumoniae</i>wild boarPCRisolation |
| spellingShingle | Rita Sárközi László Makrai László Fodor Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild Boars Pathogens <i>Actinobacillus pleuropneumoniae</i> wild boar PCR isolation |
| title | Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild Boars |
| title_full | Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild Boars |
| title_fullStr | Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild Boars |
| title_full_unstemmed | Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild Boars |
| title_short | Isolation of Biotype 1 Serotype 12 and Detection of <i>Actinobacillus pleuropneumoniae</i> from Wild Boars |
| title_sort | isolation of biotype 1 serotype 12 and detection of i actinobacillus pleuropneumoniae i from wild boars |
| topic | <i>Actinobacillus pleuropneumoniae</i> wild boar PCR isolation |
| url | https://www.mdpi.com/2076-0817/11/5/505 |
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