Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs
Abstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2021-07-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000300408&tlng=en |
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| author | Karen Araújo Magalhães Kamily Fagundes Pussi Hélton Krisman de Araújo Silvia Barbosa do Carmo Elisabete Friozi Lidiane Schultz Branquinho Manoel Sebastião da Costa Lima Junior Herintha Coeto Neitzke-Abreu |
| author_facet | Karen Araújo Magalhães Kamily Fagundes Pussi Hélton Krisman de Araújo Silvia Barbosa do Carmo Elisabete Friozi Lidiane Schultz Branquinho Manoel Sebastião da Costa Lima Junior Herintha Coeto Neitzke-Abreu |
| author_sort | Karen Araújo Magalhães |
| collection | DOAJ |
| description | Abstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the positivity of polymerase chain reaction (PCR) for the diagnosis of canine leishmaniasis in conjunctiva samples. DNA extraction was performed using SDS 20% and PCR was performed using 13A/13B primers that amplify 120-bp of Leishmania kDNA. Of the 77 dogs analyzed, 50 (64.93%) had ocular changes: 25 (32.47%) dogs had periocular lesion, 41 (53.25%) dogs had purulent eye discharge, and 17 (22.08%) dogs had both signals. PCR was positive in 35 dogs (45.45%), and there was no significant difference between dogs with and without ocular signals (p=0.4074). PCR positivity was significant higher in dogs without periocular injury (p=0.0018). Conjunctive PCR, a less invasive, fast, and painless collection technique, is indicated to complement the diagnosis, especially in dogs without periocular injury, independent of the presence of purulent eye discharge. |
| first_indexed | 2024-12-20T07:59:24Z |
| format | Article |
| id | doaj.art-ae8579f1878545ee84eb07c39e0d7342 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-20T07:59:24Z |
| publishDate | 2021-07-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-ae8579f1878545ee84eb07c39e0d73422022-12-21T19:47:33ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612021-07-0130310.1590/s1984-29612021061Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogsKaren Araújo MagalhãesKamily Fagundes PussiHélton Krisman de AraújoSilvia Barbosa do CarmoElisabete FrioziLidiane Schultz BranquinhoManoel Sebastião da Costa Lima Juniorhttps://orcid.org/0000-0003-0146-7123Herintha Coeto Neitzke-Abreuhttps://orcid.org/0000-0001-5848-670XAbstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the positivity of polymerase chain reaction (PCR) for the diagnosis of canine leishmaniasis in conjunctiva samples. DNA extraction was performed using SDS 20% and PCR was performed using 13A/13B primers that amplify 120-bp of Leishmania kDNA. Of the 77 dogs analyzed, 50 (64.93%) had ocular changes: 25 (32.47%) dogs had periocular lesion, 41 (53.25%) dogs had purulent eye discharge, and 17 (22.08%) dogs had both signals. PCR was positive in 35 dogs (45.45%), and there was no significant difference between dogs with and without ocular signals (p=0.4074). PCR positivity was significant higher in dogs without periocular injury (p=0.0018). Conjunctive PCR, a less invasive, fast, and painless collection technique, is indicated to complement the diagnosis, especially in dogs without periocular injury, independent of the presence of purulent eye discharge.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000300408&tlng=enLeishmaniaconjunctivaPCR |
| spellingShingle | Karen Araújo Magalhães Kamily Fagundes Pussi Hélton Krisman de Araújo Silvia Barbosa do Carmo Elisabete Friozi Lidiane Schultz Branquinho Manoel Sebastião da Costa Lima Junior Herintha Coeto Neitzke-Abreu Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs Revista Brasileira de Parasitologia Veterinária Leishmania conjunctiva PCR |
| title | Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs |
| title_full | Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs |
| title_fullStr | Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs |
| title_full_unstemmed | Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs |
| title_short | Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs |
| title_sort | polymerase chain reaction using conjunctival swab samples for detecting leishmania dna in dogs |
| topic | Leishmania conjunctiva PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000300408&tlng=en |
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