Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes
Pathogenic fungi and oomycetes give rise to a significant number of animal and plant diseases. While the spread of these pathogenic microorganisms is increasing globally, emerging resistance to antifungal drugs is making associated diseases more difficult to treat. High-throughput screening (HTS) an...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/12/6/639 |
| _version_ | 1797532006001672192 |
|---|---|
| author | Yiling Sun Ayelen Tayagui Sarah Sale Debolina Sarkar Volker Nock Ashley Garrill |
| author_facet | Yiling Sun Ayelen Tayagui Sarah Sale Debolina Sarkar Volker Nock Ashley Garrill |
| author_sort | Yiling Sun |
| collection | DOAJ |
| description | Pathogenic fungi and oomycetes give rise to a significant number of animal and plant diseases. While the spread of these pathogenic microorganisms is increasing globally, emerging resistance to antifungal drugs is making associated diseases more difficult to treat. High-throughput screening (HTS) and new developments in lab-on-a-chip (LOC) platforms promise to aid the discovery of urgently required new control strategies and anti-fungal/oomycete drugs. In this review, we summarize existing HTS and emergent LOC approaches in the context of infection strategies and invasive growth exhibited by these microorganisms. To aid this, we introduce key biological aspects and review existing HTS platforms based on both conventional and LOC techniques. We then provide an in-depth discussion of more specialized LOC platforms for force measurements on hyphae and to study electro- and chemotaxis in spores, approaches which have the potential to aid the discovery of alternative drug targets on future HTS platforms. Finally, we conclude with a brief discussion of the technical developments required to improve the uptake of these platforms into the general laboratory environment. |
| first_indexed | 2024-03-10T10:52:53Z |
| format | Article |
| id | doaj.art-ae881c6f05824dfba3f02463ae0aa959 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T10:52:53Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-ae881c6f05824dfba3f02463ae0aa9592023-11-21T22:07:36ZengMDPI AGMicromachines2072-666X2021-05-0112663910.3390/mi12060639Platforms for High-Throughput Screening and Force Measurements on Fungi and OomycetesYiling Sun0Ayelen Tayagui1Sarah Sale2Debolina Sarkar3Volker Nock4Ashley Garrill5Biomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New ZealandBiomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New ZealandBiomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New ZealandBiomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New ZealandBiomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New ZealandBiomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New ZealandPathogenic fungi and oomycetes give rise to a significant number of animal and plant diseases. While the spread of these pathogenic microorganisms is increasing globally, emerging resistance to antifungal drugs is making associated diseases more difficult to treat. High-throughput screening (HTS) and new developments in lab-on-a-chip (LOC) platforms promise to aid the discovery of urgently required new control strategies and anti-fungal/oomycete drugs. In this review, we summarize existing HTS and emergent LOC approaches in the context of infection strategies and invasive growth exhibited by these microorganisms. To aid this, we introduce key biological aspects and review existing HTS platforms based on both conventional and LOC techniques. We then provide an in-depth discussion of more specialized LOC platforms for force measurements on hyphae and to study electro- and chemotaxis in spores, approaches which have the potential to aid the discovery of alternative drug targets on future HTS platforms. Finally, we conclude with a brief discussion of the technical developments required to improve the uptake of these platforms into the general laboratory environment.https://www.mdpi.com/2072-666X/12/6/639fungioomyceteslab-on-a-chiphigh-throughput screeningforce measurement |
| spellingShingle | Yiling Sun Ayelen Tayagui Sarah Sale Debolina Sarkar Volker Nock Ashley Garrill Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes Micromachines fungi oomycetes lab-on-a-chip high-throughput screening force measurement |
| title | Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes |
| title_full | Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes |
| title_fullStr | Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes |
| title_full_unstemmed | Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes |
| title_short | Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes |
| title_sort | platforms for high throughput screening and force measurements on fungi and oomycetes |
| topic | fungi oomycetes lab-on-a-chip high-throughput screening force measurement |
| url | https://www.mdpi.com/2072-666X/12/6/639 |
| work_keys_str_mv | AT yilingsun platformsforhighthroughputscreeningandforcemeasurementsonfungiandoomycetes AT ayelentayagui platformsforhighthroughputscreeningandforcemeasurementsonfungiandoomycetes AT sarahsale platformsforhighthroughputscreeningandforcemeasurementsonfungiandoomycetes AT debolinasarkar platformsforhighthroughputscreeningandforcemeasurementsonfungiandoomycetes AT volkernock platformsforhighthroughputscreeningandforcemeasurementsonfungiandoomycetes AT ashleygarrill platformsforhighthroughputscreeningandforcemeasurementsonfungiandoomycetes |