Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1
Background: Ginsenoside Rg3 is a promising anticancer agent. It is usually produced by heat treatment of ginseng, in which ginsenoside Rb1 is the major ginsenoside. A kinetic study was conducted to optimize ginsenoside Rg3 production by the heat treatment of ginsenoside Rb1. Methods: Ginsenoside Rb1...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845315000135 |
| _version_ | 1819259662588968960 |
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| author | Hoang Tung Vo Jae Youl Cho Yong-Eui Choi Yong-Soon Choi Yeon-Ho Jeong |
| author_facet | Hoang Tung Vo Jae Youl Cho Yong-Eui Choi Yong-Soon Choi Yeon-Ho Jeong |
| author_sort | Hoang Tung Vo |
| collection | DOAJ |
| description | Background: Ginsenoside Rg3 is a promising anticancer agent. It is usually produced by heat treatment of ginseng, in which ginsenoside Rb1 is the major ginsenoside. A kinetic study was conducted to optimize ginsenoside Rg3 production by the heat treatment of ginsenoside Rb1.
Methods: Ginsenoside Rb1 was heated using an isothermal machine at 80°C and 100°C and analyzed using HPLC. The kinetic parameters were calculated from the experimental results. The activation energy was estimated and used to simulate the process. The optimized parameters of ginsenoside Rg3 production are suggested based on the simulation.
Results: The rate constants were 0.013 h−1 and 0.073 h−1 for the degradation of ginsenosides Rb1 and Rg3 at 80°C, respectively. The corresponding rate constants at 100°C were 0.045 h−1 and 0.155 h−1. The estimated activation energies of degradation of ginsenosides Rb1 and Rg3 were 69.2 kJ/mol and 40.9 kJ/mol, respectively. The rate constants at different temperatures were evaluated using the estimated activation energies, and the kinetic profiles of ginsenosides Rb1 and Rg3 at each temperature were simulated based on the proposed kinetic model of consecutive reaction. The optimum strategies for producing ginsenoside Rg3 from ginsenoside Rb1 are suggested based on the simulation. With increased temperature, a high concentration of ginsenoside Rg3 is formed rapidly. However, the concentration decreases quickly after the reaching the maximal concentration value.
Conclusion: The optimum temperature for producing ginsenoside Rg3 should be the highest temperature technically feasible below 180°C, in consideration of the cooling time. The optimum reaction time for heat treatment is 30 min. |
| first_indexed | 2024-12-23T19:13:35Z |
| format | Article |
| id | doaj.art-ae89848f1ab348c1afa461ae75539fe9 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-23T19:13:35Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-ae89848f1ab348c1afa461ae75539fe92022-12-21T17:34:22ZengElsevierJournal of Ginseng Research1226-84532015-10-0139430431310.1016/j.jgr.2015.02.003Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1Hoang Tung Vo0Jae Youl Cho1Yong-Eui Choi2Yong-Soon Choi3Yeon-Ho Jeong4Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, KoreaDepartment of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, KoreaDepartment of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, KoreaDepartment of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, KoreaBackground: Ginsenoside Rg3 is a promising anticancer agent. It is usually produced by heat treatment of ginseng, in which ginsenoside Rb1 is the major ginsenoside. A kinetic study was conducted to optimize ginsenoside Rg3 production by the heat treatment of ginsenoside Rb1. Methods: Ginsenoside Rb1 was heated using an isothermal machine at 80°C and 100°C and analyzed using HPLC. The kinetic parameters were calculated from the experimental results. The activation energy was estimated and used to simulate the process. The optimized parameters of ginsenoside Rg3 production are suggested based on the simulation. Results: The rate constants were 0.013 h−1 and 0.073 h−1 for the degradation of ginsenosides Rb1 and Rg3 at 80°C, respectively. The corresponding rate constants at 100°C were 0.045 h−1 and 0.155 h−1. The estimated activation energies of degradation of ginsenosides Rb1 and Rg3 were 69.2 kJ/mol and 40.9 kJ/mol, respectively. The rate constants at different temperatures were evaluated using the estimated activation energies, and the kinetic profiles of ginsenosides Rb1 and Rg3 at each temperature were simulated based on the proposed kinetic model of consecutive reaction. The optimum strategies for producing ginsenoside Rg3 from ginsenoside Rb1 are suggested based on the simulation. With increased temperature, a high concentration of ginsenoside Rg3 is formed rapidly. However, the concentration decreases quickly after the reaching the maximal concentration value. Conclusion: The optimum temperature for producing ginsenoside Rg3 should be the highest temperature technically feasible below 180°C, in consideration of the cooling time. The optimum reaction time for heat treatment is 30 min.http://www.sciencedirect.com/science/article/pii/S1226845315000135ginsenoside Rg3heatkineticsoptimizationsimulation |
| spellingShingle | Hoang Tung Vo Jae Youl Cho Yong-Eui Choi Yong-Soon Choi Yeon-Ho Jeong Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1 Journal of Ginseng Research ginsenoside Rg3 heat kinetics optimization simulation |
| title | Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1 |
| title_full | Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1 |
| title_fullStr | Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1 |
| title_full_unstemmed | Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1 |
| title_short | Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1 |
| title_sort | kinetic study for the optimization of ginsenoside rg3 production by heat treatment of ginsenoside rb1 |
| topic | ginsenoside Rg3 heat kinetics optimization simulation |
| url | http://www.sciencedirect.com/science/article/pii/S1226845315000135 |
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