Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions
Abstract Nisin is a natural bacteriocin that exhibits good antibacterial activity against Gram-positive bacteria. It has good solubility, stability, and activity under acidic conditions, but it becomes less soluble, stable, and active when the solution pH exceeds 6.0, which severely restricted the i...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-05-01
|
| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-023-00181-7 |
| _version_ | 1797822620042788864 |
|---|---|
| author | Yao Hu Kequan Xing Xiaojing Li Shangyuan Sang David Julian McClements Long Chen Jie Long Aiquan Jiao Xueming Xu Jinpeng Wang Zhengyu Jin Chao Qiu |
| author_facet | Yao Hu Kequan Xing Xiaojing Li Shangyuan Sang David Julian McClements Long Chen Jie Long Aiquan Jiao Xueming Xu Jinpeng Wang Zhengyu Jin Chao Qiu |
| author_sort | Yao Hu |
| collection | DOAJ |
| description | Abstract Nisin is a natural bacteriocin that exhibits good antibacterial activity against Gram-positive bacteria. It has good solubility, stability, and activity under acidic conditions, but it becomes less soluble, stable, and active when the solution pH exceeds 6.0, which severely restricted the industrial application range of nisin as antibacterial agent. In this study, we investigated the potential of complexing nisin with a cyclodextrin carboxylate, succinic acid-β-cyclodextrin (SACD), to overcome the disadvantages. Strong hydrogen bonding was shown between the nisin and SACD, promoting the formation of nisin-SACD complexes. These complexes exhibited good solubility under neutral and alkaline conditions, and good stability after being held at high pH values during processing with high-steam sterilization. Moreover, the nisin-SACD complexes displayed significantly improved antibacterial activity against model Gram-positive bacteria (S. aureus). This study shows that complexation can improve the efficacy of nisin under neutral and alkaline situations, which may greatly broaden its application range in food, medical, and other industries. |
| first_indexed | 2024-03-13T10:11:48Z |
| format | Article |
| id | doaj.art-ae8a03ff3208422c82051f7cf2cbfd0f |
| institution | Directory Open Access Journal |
| issn | 2396-8370 |
| language | English |
| last_indexed | 2024-03-13T10:11:48Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj.art-ae8a03ff3208422c82051f7cf2cbfd0f2023-05-21T11:28:19ZengNature Portfolionpj Science of Food2396-83702023-05-01711910.1038/s41538-023-00181-7Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditionsYao Hu0Kequan Xing1Xiaojing Li2Shangyuan Sang3David Julian McClements4Long Chen5Jie Long6Aiquan Jiao7Xueming Xu8Jinpeng Wang9Zhengyu Jin10Chao Qiu11State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityCollege of Light Industry and Food Engineering, Nanjing Forestry UniversityCollege of Food and Pharmaceutical Sciences, Ningbo UniversityDepartment of Food Science, University of MassachusettsState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, China-Canada Joint Lab of Food Nutrition and Health (Beijing), School of Food and Health, Beijing Technology and Business University (BTBU)State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan UniversityAbstract Nisin is a natural bacteriocin that exhibits good antibacterial activity against Gram-positive bacteria. It has good solubility, stability, and activity under acidic conditions, but it becomes less soluble, stable, and active when the solution pH exceeds 6.0, which severely restricted the industrial application range of nisin as antibacterial agent. In this study, we investigated the potential of complexing nisin with a cyclodextrin carboxylate, succinic acid-β-cyclodextrin (SACD), to overcome the disadvantages. Strong hydrogen bonding was shown between the nisin and SACD, promoting the formation of nisin-SACD complexes. These complexes exhibited good solubility under neutral and alkaline conditions, and good stability after being held at high pH values during processing with high-steam sterilization. Moreover, the nisin-SACD complexes displayed significantly improved antibacterial activity against model Gram-positive bacteria (S. aureus). This study shows that complexation can improve the efficacy of nisin under neutral and alkaline situations, which may greatly broaden its application range in food, medical, and other industries.https://doi.org/10.1038/s41538-023-00181-7 |
| spellingShingle | Yao Hu Kequan Xing Xiaojing Li Shangyuan Sang David Julian McClements Long Chen Jie Long Aiquan Jiao Xueming Xu Jinpeng Wang Zhengyu Jin Chao Qiu Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions npj Science of Food |
| title | Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions |
| title_full | Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions |
| title_fullStr | Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions |
| title_full_unstemmed | Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions |
| title_short | Cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions |
| title_sort | cyclodextrin carboxylate improves the stability and activity of nisin in a wider range of application conditions |
| url | https://doi.org/10.1038/s41538-023-00181-7 |
| work_keys_str_mv | AT yaohu cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT kequanxing cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT xiaojingli cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT shangyuansang cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT davidjulianmcclements cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT longchen cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT jielong cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT aiquanjiao cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT xuemingxu cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT jinpengwang cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT zhengyujin cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions AT chaoqiu cyclodextrincarboxylateimprovesthestabilityandactivityofnisininawiderrangeofapplicationconditions |