The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats
Background and Objective: Type 1 diabetes (T1D) is highly prevalent in the group of autoimmune and inflammatory patients. Tumor necrosis factor-alpha (TNF-a) and interleukin-10 (IL-10) plays an important role in regulating complex interactions between pancreatic beta cells and immune cells in the de...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2022-03-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/article-1-3983-en.html |
| _version_ | 1828465498502725632 |
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| author | Soren Valafar Eidy Alijani Fariba Aghaei Mahsa Mohsenzadeh |
| author_facet | Soren Valafar Eidy Alijani Fariba Aghaei Mahsa Mohsenzadeh |
| author_sort | Soren Valafar |
| collection | DOAJ |
| description | Background and Objective: Type 1 diabetes (T1D) is highly prevalent in the group of autoimmune and inflammatory patients. Tumor necrosis factor-alpha (TNF-a) and interleukin-10 (IL-10) plays an important role in regulating complex interactions between pancreatic beta cells and immune cells in the development of T1D. This study was performed to determine the simultaneous effect of resistance training and endothelial progenitor cell injection on blood glucose levels and protein expression of proinflammatory factors TNF-a and IL-10 in muscle tissue of streptozotocin (STZ) induced diabetic male rats.
Methods: In this experimental study, 36 male Wistar rats weighing approximately 200±20 g and six weeks old were randomly divided into six groups. Induction of diabetes was performed by intraperitoneal injection of STZ at a dose of 45 mg/kg body weight. Groups included diabetes + stem cell injection + resistance training group, diabetes + resistance training group, diabetes + stem cell injection group, control diabetic group to control the passage of time, and healthy basal and diabetic groups for defaults. Exercises were performed for 17 sessions of resistance training, including climbing ladders with increasing weight three days a week in the same laboratory conditions. Endothelial progenitor cells were cultured by femoral bone marrow aspiration and culture and then injection into the tail vein. 68 hours after the last training session, blood glucose levels were assessed by ELISA and the expression of TNF-a and IL-10 protein in muscle tissue was assessed by Western blotting.
Results: Endothelial stem cell injection, resistance training and resistance training with the simultaneous injection of endothelial stem cells significantly increased the anti-inflammatory factor IL-10 in the skeletal muscle tissue of diabetic rats in compared to control group (P<0.05). Expression of the anti-inflammatory factor IL-10 in the skeletal muscle tissue was significantly increased in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05). Glucose concentration in the skeletal muscle tissue was significantly reduced in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05).
Conclusion: It seems that 17 sessions of resistance training reduces blood glucose level and improves inflammatory conditions in response to an increase in IL-10 and a decrease in TNF-a in a group of diabetic rats with resistance training and simultaneous injection of endothelial progenitor cells in diabetic male rats. |
| first_indexed | 2024-12-11T03:33:56Z |
| format | Article |
| id | doaj.art-ae8dca8d78f149bebca4929d2ea0cdd8 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-12-11T03:33:56Z |
| publishDate | 2022-03-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-ae8dca8d78f149bebca4929d2ea0cdd82022-12-22T01:22:19ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802022-03-012414452The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male RatsSoren Valafar0Eidy Alijani1Fariba Aghaei2Mahsa Mohsenzadeh3 Ph.D Candidate in Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran. Professor of Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran. Assistant Professor of Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran. Assistant Professor of Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran. Background and Objective: Type 1 diabetes (T1D) is highly prevalent in the group of autoimmune and inflammatory patients. Tumor necrosis factor-alpha (TNF-a) and interleukin-10 (IL-10) plays an important role in regulating complex interactions between pancreatic beta cells and immune cells in the development of T1D. This study was performed to determine the simultaneous effect of resistance training and endothelial progenitor cell injection on blood glucose levels and protein expression of proinflammatory factors TNF-a and IL-10 in muscle tissue of streptozotocin (STZ) induced diabetic male rats. Methods: In this experimental study, 36 male Wistar rats weighing approximately 200±20 g and six weeks old were randomly divided into six groups. Induction of diabetes was performed by intraperitoneal injection of STZ at a dose of 45 mg/kg body weight. Groups included diabetes + stem cell injection + resistance training group, diabetes + resistance training group, diabetes + stem cell injection group, control diabetic group to control the passage of time, and healthy basal and diabetic groups for defaults. Exercises were performed for 17 sessions of resistance training, including climbing ladders with increasing weight three days a week in the same laboratory conditions. Endothelial progenitor cells were cultured by femoral bone marrow aspiration and culture and then injection into the tail vein. 68 hours after the last training session, blood glucose levels were assessed by ELISA and the expression of TNF-a and IL-10 protein in muscle tissue was assessed by Western blotting. Results: Endothelial stem cell injection, resistance training and resistance training with the simultaneous injection of endothelial stem cells significantly increased the anti-inflammatory factor IL-10 in the skeletal muscle tissue of diabetic rats in compared to control group (P<0.05). Expression of the anti-inflammatory factor IL-10 in the skeletal muscle tissue was significantly increased in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05). Glucose concentration in the skeletal muscle tissue was significantly reduced in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05). Conclusion: It seems that 17 sessions of resistance training reduces blood glucose level and improves inflammatory conditions in response to an increase in IL-10 and a decrease in TNF-a in a group of diabetic rats with resistance training and simultaneous injection of endothelial progenitor cells in diabetic male rats.http://goums.ac.ir/journal/article-1-3983-en.htmlresistance trainingendothelial progenitor cellstype 1 diabetes mellitusblood glucosetumor necrosis factor-alphainterleukin-10 |
| spellingShingle | Soren Valafar Eidy Alijani Fariba Aghaei Mahsa Mohsenzadeh The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats مجله دانشگاه علوم پزشکی گرگان resistance training endothelial progenitor cells type 1 diabetes mellitus blood glucose tumor necrosis factor-alpha interleukin-10 |
| title | The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats |
| title_full | The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats |
| title_fullStr | The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats |
| title_full_unstemmed | The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats |
| title_short | The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats |
| title_sort | simultaneous effect of resistance training and endothelial progenitor cells injection on blood glucose level tnf α and il 10 protein expression of muscular cells of diabetic male rats |
| topic | resistance training endothelial progenitor cells type 1 diabetes mellitus blood glucose tumor necrosis factor-alpha interleukin-10 |
| url | http://goums.ac.ir/journal/article-1-3983-en.html |
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