Molecular approach for identification of Catla catla using mitochondrial CO1 from Pakistan
DNA barcoding is a rapid, precise, and effective way of species identification. A short and standard target gene marker is used to create sequence profile of identified species. Specific tag or marker is used, which is derived from mitochondrial COI for identification. Effectiveness of this method a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-07-01
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| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2020.1768913 |
| _version_ | 1797639234400550912 |
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| author | Zara Naeem Samrah Masud Shoaib Hassan Muhammad Naeem |
| author_facet | Zara Naeem Samrah Masud Shoaib Hassan Muhammad Naeem |
| author_sort | Zara Naeem |
| collection | DOAJ |
| description | DNA barcoding is a rapid, precise, and effective way of species identification. A short and standard target gene marker is used to create sequence profile of identified species. Specific tag or marker is used, which is derived from mitochondrial COI for identification. Effectiveness of this method axes the degree of divergence among species. Identification is necessary for their representation. In the present work, Catla catla was used to study by using Cytochrome C Oxidase 1.The genetic distances were computed, and Neighbor Joining tree was constructed based on the Kimura 2 Parameter method. GenBank and BOLD revealed definitive identity matches. Conspecific and congeneric K2P nucleotide divergence was estimated. Evolutionary tree was analyzed clearly by relating their species to phylogenetic tree, as same as species were bunched under same tree node, while species were differently clustered under distinct nodes. These findings conclude that the gene sequence may serve as a milestone for identification and phylogenetic history of related species at molecular level. |
| first_indexed | 2024-03-11T13:14:57Z |
| format | Article |
| id | doaj.art-ae8e126baedb49e497bfd38173bf0f8b |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:14:57Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-ae8e126baedb49e497bfd38173bf0f8b2023-11-03T13:50:21ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592020-07-01533000300310.1080/23802359.2020.17689131768913Molecular approach for identification of Catla catla using mitochondrial CO1 from PakistanZara Naeem0Samrah Masud1Shoaib Hassan2Muhammad Naeem3Institute of Pure and Applied Biology, Bahauddin Zakariya UniversityInstitute of Pure and Applied Biology, Bahauddin Zakariya UniversityInstitute of Pure and Applied Biology, Bahauddin Zakariya UniversityInstitute of Pure and Applied Biology, Bahauddin Zakariya UniversityDNA barcoding is a rapid, precise, and effective way of species identification. A short and standard target gene marker is used to create sequence profile of identified species. Specific tag or marker is used, which is derived from mitochondrial COI for identification. Effectiveness of this method axes the degree of divergence among species. Identification is necessary for their representation. In the present work, Catla catla was used to study by using Cytochrome C Oxidase 1.The genetic distances were computed, and Neighbor Joining tree was constructed based on the Kimura 2 Parameter method. GenBank and BOLD revealed definitive identity matches. Conspecific and congeneric K2P nucleotide divergence was estimated. Evolutionary tree was analyzed clearly by relating their species to phylogenetic tree, as same as species were bunched under same tree node, while species were differently clustered under distinct nodes. These findings conclude that the gene sequence may serve as a milestone for identification and phylogenetic history of related species at molecular level.http://dx.doi.org/10.1080/23802359.2020.1768913catla catlacoidna barcodingidentification |
| spellingShingle | Zara Naeem Samrah Masud Shoaib Hassan Muhammad Naeem Molecular approach for identification of Catla catla using mitochondrial CO1 from Pakistan Mitochondrial DNA. Part B. Resources catla catla coi dna barcoding identification |
| title | Molecular approach for identification of Catla catla using mitochondrial CO1 from Pakistan |
| title_full | Molecular approach for identification of Catla catla using mitochondrial CO1 from Pakistan |
| title_fullStr | Molecular approach for identification of Catla catla using mitochondrial CO1 from Pakistan |
| title_full_unstemmed | Molecular approach for identification of Catla catla using mitochondrial CO1 from Pakistan |
| title_short | Molecular approach for identification of Catla catla using mitochondrial CO1 from Pakistan |
| title_sort | molecular approach for identification of catla catla using mitochondrial co1 from pakistan |
| topic | catla catla coi dna barcoding identification |
| url | http://dx.doi.org/10.1080/23802359.2020.1768913 |
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