Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes
The immunotoxicological effects of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) mixtures on <em>Eisenia andrei </em>earthworms have never been studied. In this work we investigated these effects both for <em>in vitro </em>and <em>in vivo</em> exposur...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-10-01
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| Series: | Journal of Xenobiotics |
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| Online Access: | http://www.pagepressjournals.org/index.php/xeno/article/view/95 |
| _version_ | 1798025108555563008 |
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| author | Hayet Belmeskine Pauline Brousseau Sami Haddad Louise Vandelac Michel Fournier |
| author_facet | Hayet Belmeskine Pauline Brousseau Sami Haddad Louise Vandelac Michel Fournier |
| author_sort | Hayet Belmeskine |
| collection | DOAJ |
| description | The immunotoxicological effects of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) mixtures on <em>Eisenia andrei </em>earthworms have never been studied. In this work we investigated these effects both for <em>in vitro </em>and <em>in vivo</em> exposure, using the viability and the phagocytic activity of coelomocytes as immunological biomarkers and the flow cytometry was used for analysis. The <em>in vitro </em>exposure revealed a cytotoxic effect of PCDD/Fs mixture (C2) containing 50¥10-3 ng/mL of 2, 3, 7, 8-TCDD and an induction of the phagocytic capacity at the mixture (C1) containing 25¥10-3 ng/mL of 2, 3, 7, 8-TCDD. In the <em>in vivo </em>filter paper exposure, the immunocompetence of earthworms was assessed after 3 h-exposure to mixtures of PCDD/Fs at the levels of C1, C2, C3 and C4 containing about; 0.05, 0.3, 0.5 and 0.83 ng of 2, 3, 7, 8-TCDD/cm², respectively. Morphological observations showed an excessive secretion of mucus and body surface lesions in worms exposed to higher concentrations (C3 and C4), which revealed that these organisms were affected by PCDD/Fs either through skin and/or by feeding. The levels of the extruded cell yield decreased significantly at all the concentrations tested. However, the cell viability was shown to be unaffected by PCDD/Fs concentrations. It was also shown, that exposure to the highest PCDD/Fs concentrations; C2, C3 and C4 inhibited both phagocytic activity and efficiency. |
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| issn | 2039-4705 2039-4713 |
| language | English |
| last_indexed | 2024-04-11T18:13:32Z |
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| spelling | doaj.art-ae9190f6ff5f4d21ad01cbbfbbe2ae9d2022-12-22T04:10:01ZengMDPI AGJournal of Xenobiotics2039-47052039-47132011-10-0111e6e610.4081/xeno.2011.e654Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytesHayet Belmeskine0Pauline Brousseau1Sami Haddad2Louise Vandelac3Michel Fournier4INRS-Institut Armand Frappier, Laval, QuébecINRS-Institut Armand Frappier, Laval, QuébecDépartement de santé environnementale et santé au travail, Université de Montréal, QuebecDépartement des sciences de l’environnement de l’université du Québec à Montréal, Montréal, QuébecINRS-Institut Armand Frappier, Laval, QuébecThe immunotoxicological effects of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) mixtures on <em>Eisenia andrei </em>earthworms have never been studied. In this work we investigated these effects both for <em>in vitro </em>and <em>in vivo</em> exposure, using the viability and the phagocytic activity of coelomocytes as immunological biomarkers and the flow cytometry was used for analysis. The <em>in vitro </em>exposure revealed a cytotoxic effect of PCDD/Fs mixture (C2) containing 50¥10-3 ng/mL of 2, 3, 7, 8-TCDD and an induction of the phagocytic capacity at the mixture (C1) containing 25¥10-3 ng/mL of 2, 3, 7, 8-TCDD. In the <em>in vivo </em>filter paper exposure, the immunocompetence of earthworms was assessed after 3 h-exposure to mixtures of PCDD/Fs at the levels of C1, C2, C3 and C4 containing about; 0.05, 0.3, 0.5 and 0.83 ng of 2, 3, 7, 8-TCDD/cm², respectively. Morphological observations showed an excessive secretion of mucus and body surface lesions in worms exposed to higher concentrations (C3 and C4), which revealed that these organisms were affected by PCDD/Fs either through skin and/or by feeding. The levels of the extruded cell yield decreased significantly at all the concentrations tested. However, the cell viability was shown to be unaffected by PCDD/Fs concentrations. It was also shown, that exposure to the highest PCDD/Fs concentrations; C2, C3 and C4 inhibited both phagocytic activity and efficiency.http://www.pagepressjournals.org/index.php/xeno/article/view/95PCDDs/Fs, earthworms, Eisenia andrei, immunosuppression, phagocytosis, coelomocytes. |
| spellingShingle | Hayet Belmeskine Pauline Brousseau Sami Haddad Louise Vandelac Michel Fournier Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes Journal of Xenobiotics PCDDs/Fs, earthworms, Eisenia andrei, immunosuppression, phagocytosis, coelomocytes. |
| title | Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes |
| title_full | Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes |
| title_fullStr | Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes |
| title_full_unstemmed | Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes |
| title_short | Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes |
| title_sort | effects of polychlorinated dibenzo p dioxins and polychlorinated dibenzofurans on phagocytic response of eisenia andrei coelomocytes |
| topic | PCDDs/Fs, earthworms, Eisenia andrei, immunosuppression, phagocytosis, coelomocytes. |
| url | http://www.pagepressjournals.org/index.php/xeno/article/view/95 |
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