The growth of dental pulp stem cells in portland cement micro-environment
Background: Portland cement (PC), the base material of mineral trioxide aggregate has been applied in vivo and in vitro studies, and showed promising physical and mechanical properties on several cell lineages but human dental pulp stem cells (DPSCs), known as mesenchymal stem cells with their multi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Journal of Medical Sciences |
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| Online Access: | http://jms.ndmctsgh.edu.tw/article.asp?issn=1011-4564;year=2015;volume=35;issue=2;spage=62;epage=67;aulast=Wang |
| _version_ | 1819212038866468864 |
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| author | Shih-Min Wang Chia-Hsuan Lee Chih-Hung Lin Gunng-Shinng Chen Jiang-Chuan Liu Chung-Hsing Li |
| author_facet | Shih-Min Wang Chia-Hsuan Lee Chih-Hung Lin Gunng-Shinng Chen Jiang-Chuan Liu Chung-Hsing Li |
| author_sort | Shih-Min Wang |
| collection | DOAJ |
| description | Background: Portland cement (PC), the base material of mineral trioxide aggregate has been applied in vivo and in vitro studies, and showed promising physical and mechanical properties on several cell lineages but human dental pulp stem cells (DPSCs), known as mesenchymal stem cells with their multipotency. Our study aims to evaluate the cytotoxicity of PC mixing with distilled water (Td) or normal saline (Tn) on DPSCs. Materials and Methods: DPSCs were isolated from pulp tissue and identified before viability assay. DPSCs were treated with the concentration of 100%, 50%, 25%, 12.5% and 6.25% extracts in Td and Tn. Cell viability was evaluate after 24 h, 48 h, 72 h and 7 days treating. Cells were analyzed for cell surface antigen expression by flow cytometry. The pH levels of extracts were detected by pH meter. Cell viability was determined with cell counting kit-8 assay. Results: Viability of Td and Tn showed the general trend that dropped slightly at 24 h, increased at 48 h then showed no statistical differences to control at 72 h. The viability of the concentration of 100% groups dropped gradually from 24 h to 72 h. Cell proliferation was improved in low concentration groups on 48 h and 72 h. On day 7, cell viability showed no statistical difference to control except Tn 50% (110.9%) and Tn 100% (84.0%). Conclusions: PC is probably a potential candidate for the use of pulp therapy, or further, a budding material for pulp regeneration. |
| first_indexed | 2024-12-23T06:36:37Z |
| format | Article |
| id | doaj.art-ae92766d59a34cce81d8cfc96abd5d88 |
| institution | Directory Open Access Journal |
| issn | 1011-4564 |
| language | English |
| last_indexed | 2024-12-23T06:36:37Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Medical Sciences |
| spelling | doaj.art-ae92766d59a34cce81d8cfc96abd5d882022-12-21T17:56:47ZengWolters Kluwer Medknow PublicationsJournal of Medical Sciences1011-45642015-01-01352626710.4103/1011-4564.156012The growth of dental pulp stem cells in portland cement micro-environmentShih-Min WangChia-Hsuan LeeChih-Hung LinGunng-Shinng ChenJiang-Chuan LiuChung-Hsing LiBackground: Portland cement (PC), the base material of mineral trioxide aggregate has been applied in vivo and in vitro studies, and showed promising physical and mechanical properties on several cell lineages but human dental pulp stem cells (DPSCs), known as mesenchymal stem cells with their multipotency. Our study aims to evaluate the cytotoxicity of PC mixing with distilled water (Td) or normal saline (Tn) on DPSCs. Materials and Methods: DPSCs were isolated from pulp tissue and identified before viability assay. DPSCs were treated with the concentration of 100%, 50%, 25%, 12.5% and 6.25% extracts in Td and Tn. Cell viability was evaluate after 24 h, 48 h, 72 h and 7 days treating. Cells were analyzed for cell surface antigen expression by flow cytometry. The pH levels of extracts were detected by pH meter. Cell viability was determined with cell counting kit-8 assay. Results: Viability of Td and Tn showed the general trend that dropped slightly at 24 h, increased at 48 h then showed no statistical differences to control at 72 h. The viability of the concentration of 100% groups dropped gradually from 24 h to 72 h. Cell proliferation was improved in low concentration groups on 48 h and 72 h. On day 7, cell viability showed no statistical difference to control except Tn 50% (110.9%) and Tn 100% (84.0%). Conclusions: PC is probably a potential candidate for the use of pulp therapy, or further, a budding material for pulp regeneration.http://jms.ndmctsgh.edu.tw/article.asp?issn=1011-4564;year=2015;volume=35;issue=2;spage=62;epage=67;aulast=WangPortland cementdental pulp stem cellcell viabilitypulp therapy |
| spellingShingle | Shih-Min Wang Chia-Hsuan Lee Chih-Hung Lin Gunng-Shinng Chen Jiang-Chuan Liu Chung-Hsing Li The growth of dental pulp stem cells in portland cement micro-environment Journal of Medical Sciences Portland cement dental pulp stem cell cell viability pulp therapy |
| title | The growth of dental pulp stem cells in portland cement micro-environment |
| title_full | The growth of dental pulp stem cells in portland cement micro-environment |
| title_fullStr | The growth of dental pulp stem cells in portland cement micro-environment |
| title_full_unstemmed | The growth of dental pulp stem cells in portland cement micro-environment |
| title_short | The growth of dental pulp stem cells in portland cement micro-environment |
| title_sort | growth of dental pulp stem cells in portland cement micro environment |
| topic | Portland cement dental pulp stem cell cell viability pulp therapy |
| url | http://jms.ndmctsgh.edu.tw/article.asp?issn=1011-4564;year=2015;volume=35;issue=2;spage=62;epage=67;aulast=Wang |
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