Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice
UDP-glycosyltransferases (UGTs) play key roles in modulating plant development and responses to environmental challenges. Previous research reported that the Arabidopsis UDP-glucosyltransferase 74E2 (<i>AtUGT74E2</i>), which transfers glucose to indole-3-butyric acid (IBA), is involved i...
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Ting Wang Pan Li Tianjiao Mu Guangrui Dong Chengchao Zheng Shanghui Jin Tingting Chen Bingkai Hou Yanjie Li |
| author_facet | Ting Wang Pan Li Tianjiao Mu Guangrui Dong Chengchao Zheng Shanghui Jin Tingting Chen Bingkai Hou Yanjie Li |
| author_sort | Ting Wang |
| collection | DOAJ |
| description | UDP-glycosyltransferases (UGTs) play key roles in modulating plant development and responses to environmental challenges. Previous research reported that the Arabidopsis UDP-glucosyltransferase 74E2 (<i>AtUGT74E2</i>), which transfers glucose to indole-3-butyric acid (IBA), is involved in regulating plant architecture and stress responses. Here, we show novel and distinct roles of <i>UGT74E2</i> in rice. We found that overexpression of <i>AtUGT74E2</i> in rice could enhance seed germination. This effect was also observed in the presence of IBA and abscisic acid (ABA), as well as salt and drought stresses. Further investigation indicated that the overexpression lines had lower levels of free IBA and ABA compared to wild-type plants. Auxin signaling pathway gene expression such as for <i>OsARF</i> and <i>OsGH3</i> genes, as well as ABA signaling pathway genes <i>OsABI3</i> and <i>OsABI5,</i> was substantially downregulated in germinating seeds of <i>UGT74E2</i> overexpression lines. Consistently, due to reduced IBA and ABA levels, the established seedlings were less tolerant to drought and salt stresses. The regulation of rice seed germination and stress tolerance could be attributed to IBA and ABA level alterations, as well as modulation of the auxin/ABA signaling pathways by <i>UGT74E2</i>. The distinct roles of <i>UGT74E2</i> in rice implied that complex and different molecular regulation networks exist between Arabidopsis and rice. |
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| spelling | doaj.art-ae93e5c077bb4a23bee9e1e7b964e51b2023-11-20T15:41:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012119723910.3390/ijms21197239Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in RiceTing Wang0Pan Li1Tianjiao Mu2Guangrui Dong3Chengchao Zheng4Shanghui Jin5Tingting Chen6Bingkai Hou7Yanjie Li8The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaCollege of Pharmacy, Liaocheng University, Liaocheng 252000, ChinaThe Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaSchool of Life Science, Qingdao Agricultural University, Qingdao 266109, ChinaThe Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaUDP-glycosyltransferases (UGTs) play key roles in modulating plant development and responses to environmental challenges. Previous research reported that the Arabidopsis UDP-glucosyltransferase 74E2 (<i>AtUGT74E2</i>), which transfers glucose to indole-3-butyric acid (IBA), is involved in regulating plant architecture and stress responses. Here, we show novel and distinct roles of <i>UGT74E2</i> in rice. We found that overexpression of <i>AtUGT74E2</i> in rice could enhance seed germination. This effect was also observed in the presence of IBA and abscisic acid (ABA), as well as salt and drought stresses. Further investigation indicated that the overexpression lines had lower levels of free IBA and ABA compared to wild-type plants. Auxin signaling pathway gene expression such as for <i>OsARF</i> and <i>OsGH3</i> genes, as well as ABA signaling pathway genes <i>OsABI3</i> and <i>OsABI5,</i> was substantially downregulated in germinating seeds of <i>UGT74E2</i> overexpression lines. Consistently, due to reduced IBA and ABA levels, the established seedlings were less tolerant to drought and salt stresses. The regulation of rice seed germination and stress tolerance could be attributed to IBA and ABA level alterations, as well as modulation of the auxin/ABA signaling pathways by <i>UGT74E2</i>. The distinct roles of <i>UGT74E2</i> in rice implied that complex and different molecular regulation networks exist between Arabidopsis and rice.https://www.mdpi.com/1422-0067/21/19/7239UDP-glycosyltransferasesglycosylationindole-3-butyric acidabscisic aciddroughtsalt |
| spellingShingle | Ting Wang Pan Li Tianjiao Mu Guangrui Dong Chengchao Zheng Shanghui Jin Tingting Chen Bingkai Hou Yanjie Li Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice International Journal of Molecular Sciences UDP-glycosyltransferases glycosylation indole-3-butyric acid abscisic acid drought salt |
| title | Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice |
| title_full | Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice |
| title_fullStr | Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice |
| title_full_unstemmed | Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice |
| title_short | Overexpression of <i>UGT74E2</i>, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice |
| title_sort | overexpression of i ugt74e2 i an arabidopsis iba glycosyltransferase enhances seed germination and modulates stress tolerance via aba signaling in rice |
| topic | UDP-glycosyltransferases glycosylation indole-3-butyric acid abscisic acid drought salt |
| url | https://www.mdpi.com/1422-0067/21/19/7239 |
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