Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printing
Three-dimensional scaffolds were made from a solution of poly(lactide-co-glycolide) mixed with tetraglycol using antisolvent 3D printing. The elastic properties and the structure of the obtained matrices were studied. MTT-test and staining with PKH-26, Calcein-AM, DAPI with subsequent fluorescence m...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2024-01-01
|
| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/03/bioconf_aquaculture2024_05014.pdf |
| _version_ | 1797352996826251264 |
|---|---|
| author | Mokrousova Victoria Nedorubova Irina Vasilyev Andrey Kuznetsova Valeriya Mironov Anton Khvorostina Maria Bukharova Tatyana Popov Vladimir Goldshtein Dmitry Losev Fedor Kulakov Anatoly |
| author_facet | Mokrousova Victoria Nedorubova Irina Vasilyev Andrey Kuznetsova Valeriya Mironov Anton Khvorostina Maria Bukharova Tatyana Popov Vladimir Goldshtein Dmitry Losev Fedor Kulakov Anatoly |
| author_sort | Mokrousova Victoria |
| collection | DOAJ |
| description | Three-dimensional scaffolds were made from a solution of poly(lactide-co-glycolide) mixed with tetraglycol using antisolvent 3D printing. The elastic properties and the structure of the obtained matrices were studied. MTT-test and staining with PKH-26, Calcein-AM, DAPI with subsequent fluorescence microscopy were used to study biological properties. The three-dimensional scaffolds had good mechanical properties. Young’s modulus value was 18±2 MPa, tensile strength was 0.43±0.05 MPa. The relative survival rate of cells after the first day was 99.58±2.28%, on the 14th day – 98.14±2.22%. The structure of the scaffold promoted cell adhesion and spreading on its surface. The poly(lactide-co-glycolide) matrices produced by antisolvent printing have high porosity, biocompatibility and good mechanical properties. It is allowed to use them in the future as a basis for personalized constructions for the replacement of extensive bone defects. |
| first_indexed | 2024-03-08T13:24:51Z |
| format | Article |
| id | doaj.art-ae9a849137b34b3aacf222eeb690e75a |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-03-08T13:24:51Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-ae9a849137b34b3aacf222eeb690e75a2024-01-17T15:00:15ZengEDP SciencesBIO Web of Conferences2117-44582024-01-01840501410.1051/bioconf/20248405014bioconf_aquaculture2024_05014Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printingMokrousova Victoria0Nedorubova Irina1Vasilyev Andrey2Kuznetsova Valeriya3Mironov Anton4Khvorostina Maria5Bukharova Tatyana6Popov Vladimir7Goldshtein Dmitry8Losev Fedor9Kulakov Anatoly10Central Research Institute of Dental and Maxillofacial SurgeryCentral Research Institute of Dental and Maxillofacial SurgeryCentral Research Institute of Dental and Maxillofacial SurgeryCentral Research Institute of Dental and Maxillofacial SurgeryFederal Scientific Research Center «Crystallography and photonics», Russian Academy of ScienceCentral Research Institute of Dental and Maxillofacial SurgeryCentral Research Institute of Dental and Maxillofacial SurgeryFederal Scientific Research Center «Crystallography and photonics», Russian Academy of ScienceResearch Centre for Medical GeneticsCentral Research Institute of Dental and Maxillofacial SurgeryCentral Research Institute of Dental and Maxillofacial SurgeryThree-dimensional scaffolds were made from a solution of poly(lactide-co-glycolide) mixed with tetraglycol using antisolvent 3D printing. The elastic properties and the structure of the obtained matrices were studied. MTT-test and staining with PKH-26, Calcein-AM, DAPI with subsequent fluorescence microscopy were used to study biological properties. The three-dimensional scaffolds had good mechanical properties. Young’s modulus value was 18±2 MPa, tensile strength was 0.43±0.05 MPa. The relative survival rate of cells after the first day was 99.58±2.28%, on the 14th day – 98.14±2.22%. The structure of the scaffold promoted cell adhesion and spreading on its surface. The poly(lactide-co-glycolide) matrices produced by antisolvent printing have high porosity, biocompatibility and good mechanical properties. It is allowed to use them in the future as a basis for personalized constructions for the replacement of extensive bone defects.https://www.bio-conferences.org/articles/bioconf/pdf/2024/03/bioconf_aquaculture2024_05014.pdf |
| spellingShingle | Mokrousova Victoria Nedorubova Irina Vasilyev Andrey Kuznetsova Valeriya Mironov Anton Khvorostina Maria Bukharova Tatyana Popov Vladimir Goldshtein Dmitry Losev Fedor Kulakov Anatoly Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printing BIO Web of Conferences |
| title | Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printing |
| title_full | Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printing |
| title_fullStr | Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printing |
| title_full_unstemmed | Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printing |
| title_short | Mechanical and biocompatible properties of the poly(lactide-co-glycolide) matrices produced by antisolvent 3D printing |
| title_sort | mechanical and biocompatible properties of the poly lactide co glycolide matrices produced by antisolvent 3d printing |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/03/bioconf_aquaculture2024_05014.pdf |
| work_keys_str_mv | AT mokrousovavictoria mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT nedorubovairina mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT vasilyevandrey mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT kuznetsovavaleriya mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT mironovanton mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT khvorostinamaria mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT bukharovatatyana mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT popovvladimir mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT goldshteindmitry mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT losevfedor mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting AT kulakovanatoly mechanicalandbiocompatiblepropertiesofthepolylactidecoglycolidematricesproducedbyantisolvent3dprinting |