Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restoration
Metribuzin (MB), a triazinone herbicide is extensively sprayed for weed control in agriculture, has been reported to contaminate soil, groundwater, and surface waters. In soil, MB residues can negatively affect not only the germination of subsequent crops but also disturb soil bacterial community. T...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1027284/full |
| _version_ | 1797903813499158528 |
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| author | Abdul Qadeer Wahla Samina Anwar Muhammad Irfan Fareed Wasiq Ikram Wasiq Ikram Liaqat Ali Hesham F. Alharby Hesham F. Alharby Atif A. Bamagoos Afaf A. Almaghamsi Samina Iqbal Shafaqat Ali Shafaqat Ali |
| author_facet | Abdul Qadeer Wahla Samina Anwar Muhammad Irfan Fareed Wasiq Ikram Wasiq Ikram Liaqat Ali Hesham F. Alharby Hesham F. Alharby Atif A. Bamagoos Afaf A. Almaghamsi Samina Iqbal Shafaqat Ali Shafaqat Ali |
| author_sort | Abdul Qadeer Wahla |
| collection | DOAJ |
| description | Metribuzin (MB), a triazinone herbicide is extensively sprayed for weed control in agriculture, has been reported to contaminate soil, groundwater, and surface waters. In soil, MB residues can negatively affect not only the germination of subsequent crops but also disturb soil bacterial community. The present study describes the use of biochar as a carrier material to immobilize MB-degrading bacterial consortium, for remediation of MB-contaminated soil and restoration of soil bacterial community in soil microcosms. The bacterial consortium (MB3R) comprised four bacterial strains, i.e., Rhodococcus rhodochrous AQ1, Bacillus tequilensis AQ2, Bacillus aryabhattai AQ3, and Bacillus safensis AQ4. Significantly higher MB remediation was observed in soil augmented with bacterial consortium immobilized on biochar compared to the soil augmented with un-immobilized bacterial consortium. Immobilization of MB3R on biochar resulted in higher MB degradation rate (0.017 Kd−1) and reduced half-life (40 days) compared to 0.010 Kd−1 degradation rate and 68 day half-life in treatments where un-immobilized bacterial consortium was employed. It is worth mentioning that the MB degradation products metribuzin-desamino (DA), metribuzin-diketo (DK), and metribuzin desamino-diketo (DADK) were detected in the treatments where MB3R was inoculated either alone or in combination with biochar. MB contamination significantly altered the composition of soil bacteria. However, soil bacterial community was conserved in response to augmentation with MB3R immobilized on biochar. Immobilization of the bacterial consortium MB3R on biochar can potentially be exploited for remediation of MB-contaminated soil and protecting its microbiota. |
| first_indexed | 2024-04-10T09:38:58Z |
| format | Article |
| id | doaj.art-ae9ae0584f094c89ab94f25928ca1802 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T09:38:58Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ae9ae0584f094c89ab94f25928ca18022023-02-17T14:31:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011310.3389/fmicb.2022.10272841027284Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restorationAbdul Qadeer Wahla0Samina Anwar1Muhammad Irfan Fareed2Wasiq Ikram3Wasiq Ikram4Liaqat Ali5Hesham F. Alharby6Hesham F. Alharby7Atif A. Bamagoos8Afaf A. Almaghamsi9Samina Iqbal10Shafaqat Ali11Shafaqat Ali12Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Punjab, PakistanSoil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Punjab, PakistanDepartment of Life Sciences, School of Science, University of Management and Technology, Johar Town, Lahore, PakistanSchool of Botany, Minhaj University Lahore (MUL), Lahore, PakistanSchool of Botany, Minhaj University Lahore (MUL), Lahore, PakistanDepartment of Soil and Environmental Sciences, University of Agriculture Faisalabad, Sub Campus Burewala, Vehari, PakistanDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaPlant Biology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Biology, College of Science, University of Jeddah, Jeddah, Saudi ArabiaSoil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Punjab, PakistanDepartment of Environmental Science, Government College University, Faisalabad, Pakistan0Department of Biological Sciences and Technology, China Medical University, Taichung, TaiwanMetribuzin (MB), a triazinone herbicide is extensively sprayed for weed control in agriculture, has been reported to contaminate soil, groundwater, and surface waters. In soil, MB residues can negatively affect not only the germination of subsequent crops but also disturb soil bacterial community. The present study describes the use of biochar as a carrier material to immobilize MB-degrading bacterial consortium, for remediation of MB-contaminated soil and restoration of soil bacterial community in soil microcosms. The bacterial consortium (MB3R) comprised four bacterial strains, i.e., Rhodococcus rhodochrous AQ1, Bacillus tequilensis AQ2, Bacillus aryabhattai AQ3, and Bacillus safensis AQ4. Significantly higher MB remediation was observed in soil augmented with bacterial consortium immobilized on biochar compared to the soil augmented with un-immobilized bacterial consortium. Immobilization of MB3R on biochar resulted in higher MB degradation rate (0.017 Kd−1) and reduced half-life (40 days) compared to 0.010 Kd−1 degradation rate and 68 day half-life in treatments where un-immobilized bacterial consortium was employed. It is worth mentioning that the MB degradation products metribuzin-desamino (DA), metribuzin-diketo (DK), and metribuzin desamino-diketo (DADK) were detected in the treatments where MB3R was inoculated either alone or in combination with biochar. MB contamination significantly altered the composition of soil bacteria. However, soil bacterial community was conserved in response to augmentation with MB3R immobilized on biochar. Immobilization of the bacterial consortium MB3R on biochar can potentially be exploited for remediation of MB-contaminated soil and protecting its microbiota.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1027284/fullBioaugmentationRice-husk biocharsBiodegradationMetagenomics analysisSoil Bacterial diversity |
| spellingShingle | Abdul Qadeer Wahla Samina Anwar Muhammad Irfan Fareed Wasiq Ikram Wasiq Ikram Liaqat Ali Hesham F. Alharby Hesham F. Alharby Atif A. Bamagoos Afaf A. Almaghamsi Samina Iqbal Shafaqat Ali Shafaqat Ali Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restoration Frontiers in Microbiology Bioaugmentation Rice-husk biochars Biodegradation Metagenomics analysis Soil Bacterial diversity |
| title | Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restoration |
| title_full | Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restoration |
| title_fullStr | Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restoration |
| title_full_unstemmed | Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restoration |
| title_short | Immobilization of metribuzin-degrading bacteria on biochar: Enhanced soil remediation and bacterial community restoration |
| title_sort | immobilization of metribuzin degrading bacteria on biochar enhanced soil remediation and bacterial community restoration |
| topic | Bioaugmentation Rice-husk biochars Biodegradation Metagenomics analysis Soil Bacterial diversity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1027284/full |
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