Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck
Abstract Background In the late phase of production, ducks untimely cease laying, leading to a lower feed conversion. Liver plays a vital role in the synthesis and transport of yolk materials during egg formation in birds. However, the molecular mechanism of liver in ceased-laying duck is far from c...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-09089-0 |
| _version_ | 1797973723005845504 |
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| author | Xue Du Shujing Lai Wanqiu Zhao Xiaoqin Xu Wenwu Xu Tao Zeng Yong Tian Lizhi Lu |
| author_facet | Xue Du Shujing Lai Wanqiu Zhao Xiaoqin Xu Wenwu Xu Tao Zeng Yong Tian Lizhi Lu |
| author_sort | Xue Du |
| collection | DOAJ |
| description | Abstract Background In the late phase of production, ducks untimely cease laying, leading to a lower feed conversion. Liver plays a vital role in the synthesis and transport of yolk materials during egg formation in birds. However, the molecular mechanism of liver in ceased-laying duck is far from clear, higher resolution and deeper analysis is needed. Sing-cell RNA-sequencing of 10 × Genomics platform can help to map the liver single cell gene expression atlas of Shaoxing duck and provide new insights into the liver between egg-laying and ceased-laying ducks. Results About 20,000 single cells were profiled and 22 clusters were identified. All the clusters were identified as 6 cell types. The dominant cell type is hepatocyte, accounted for about 60% of all the cells. Of note, the heterogeneity of cells between egg-laying duck and ceased-laying duck mainly occurred in hepatocytes. Cells of cluster 3 and 12 were the unique hepatocyte states of egg-laying ducks, while cells of cluster 0 and 15 were the unique hepatocyte states of ceased-laying ducks. The expression mode of yolk precursor transporters, lipid metabolizing enzymes and fibrinogens were different in hepatocytes between egg-laying duck and ceased-laying duck. APOV1, VTG2, VTG1, APOB, RBP, VTDB and SCD might be activated in egg-laying ducks, while APOA1, APOA4, APOC3, FGB and FGG might be activated in ceased-laying ducks. Conclusions Our study further proofs that APOV1 and APOB play key roles in egg production, rather than APOA1 and APOA4. It is also the first to detect a correlation between the higher expression of APOC3, FGB, FGG and ceased-laying in duck. |
| first_indexed | 2024-04-11T04:08:53Z |
| format | Article |
| id | doaj.art-ae9b6052798342b49f3b5f44c72c42c3 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-11T04:08:53Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-ae9b6052798342b49f3b5f44c72c42c32023-01-01T12:13:45ZengBMCBMC Genomics1471-21642022-12-0123111010.1186/s12864-022-09089-0Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duckXue Du0Shujing Lai1Wanqiu Zhao2Xiaoqin Xu3Wenwu Xu4Tao Zeng5Yong Tian6Lizhi Lu7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural SciencesShanghai Institute of Immunology, Shanghai Jiao Tong University School of MedicineInstitute of Horticulture, Zhejiang Academy of Agricultural SciencesInstitute of Ecology, China West Normal UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural SciencesAbstract Background In the late phase of production, ducks untimely cease laying, leading to a lower feed conversion. Liver plays a vital role in the synthesis and transport of yolk materials during egg formation in birds. However, the molecular mechanism of liver in ceased-laying duck is far from clear, higher resolution and deeper analysis is needed. Sing-cell RNA-sequencing of 10 × Genomics platform can help to map the liver single cell gene expression atlas of Shaoxing duck and provide new insights into the liver between egg-laying and ceased-laying ducks. Results About 20,000 single cells were profiled and 22 clusters were identified. All the clusters were identified as 6 cell types. The dominant cell type is hepatocyte, accounted for about 60% of all the cells. Of note, the heterogeneity of cells between egg-laying duck and ceased-laying duck mainly occurred in hepatocytes. Cells of cluster 3 and 12 were the unique hepatocyte states of egg-laying ducks, while cells of cluster 0 and 15 were the unique hepatocyte states of ceased-laying ducks. The expression mode of yolk precursor transporters, lipid metabolizing enzymes and fibrinogens were different in hepatocytes between egg-laying duck and ceased-laying duck. APOV1, VTG2, VTG1, APOB, RBP, VTDB and SCD might be activated in egg-laying ducks, while APOA1, APOA4, APOC3, FGB and FGG might be activated in ceased-laying ducks. Conclusions Our study further proofs that APOV1 and APOB play key roles in egg production, rather than APOA1 and APOA4. It is also the first to detect a correlation between the higher expression of APOC3, FGB, FGG and ceased-laying in duck.https://doi.org/10.1186/s12864-022-09089-0Ceased-laying duckSingle-cell RNA-seqCell heterogeneityHepatocytesCell cluster |
| spellingShingle | Xue Du Shujing Lai Wanqiu Zhao Xiaoqin Xu Wenwu Xu Tao Zeng Yong Tian Lizhi Lu Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck BMC Genomics Ceased-laying duck Single-cell RNA-seq Cell heterogeneity Hepatocytes Cell cluster |
| title | Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck |
| title_full | Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck |
| title_fullStr | Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck |
| title_full_unstemmed | Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck |
| title_short | Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck |
| title_sort | single cell rna sequencing revealed the liver heterogeneity between egg laying duck and ceased laying duck |
| topic | Ceased-laying duck Single-cell RNA-seq Cell heterogeneity Hepatocytes Cell cluster |
| url | https://doi.org/10.1186/s12864-022-09089-0 |
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