Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored Plant
<i>Ficus vasta</i> Forssk. (Moraceae family) is an important medicinal plant that has not been previously investigated for its phytochemical and biological potential. Phytochemical screening, total bioactive content, and GCMS analysis were used to determine its phytoconstituents profile....
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/9/1155 |
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| author | Hanan Y. Aati Mariyam Anwar Jawaher Al-Qahtani Areej Al-Taweel Kashif-ur-Rehman Khan Sultan Aati Faisal Usman Bilal Ahmad Ghalloo Hafiz Muhammad Asif Jafir Hussain Shirazi Aliza Abbasi |
| author_facet | Hanan Y. Aati Mariyam Anwar Jawaher Al-Qahtani Areej Al-Taweel Kashif-ur-Rehman Khan Sultan Aati Faisal Usman Bilal Ahmad Ghalloo Hafiz Muhammad Asif Jafir Hussain Shirazi Aliza Abbasi |
| author_sort | Hanan Y. Aati |
| collection | DOAJ |
| description | <i>Ficus vasta</i> Forssk. (Moraceae family) is an important medicinal plant that has not been previously investigated for its phytochemical and biological potential. Phytochemical screening, total bioactive content, and GCMS analysis were used to determine its phytoconstituents profile. Antioxidant, antibacterial, antifungal, anti-viral, cytotoxicity, thrombolytic, and enzyme inhibition activities were examined for biological evaluation. The plant extract exhibited the maximum total phenolic (89.47 ± 3.21 mg GAE/g) and total flavonoid contents (129.2 ± 4.14 mg QE/g), which may be related to the higher antioxidant potential of the extract. The extract showed strong α-amylase (IC<sub>50</sub> 5 ± 0.21 µg/mL) and α-glucosidase inhibition activity (IC<sub>50</sub> 5 ± 0.32 µg/mL). Significant results were observed in the case of antibacterial, antifungal, and anti-viral activities. The <i>F. vasta</i> extract inhibited the growth of HepG2 cells in a dose-dependent manner. The GCMS analysis of the extract provided the preliminary identification of 28 phytocompounds. In addition, the compounds identified by GCMS were subjected to in silico molecular docking analysis in order to identify any interactions between the compounds and enzymes (α-amylase and α-glucosidase). After that, the best-docked compounds were subjected to ADMET studies which provide information on pharmacokinetics, drug-likeness, physicochemical properties, and toxicity. The present study highlighted that the ethanol extract of <i>F. vasta</i> has antidiabetic, antimicrobial, anti-viral, and anti-cancer potentials that can be further explored for novel drug development. |
| first_indexed | 2024-03-10T00:56:01Z |
| format | Article |
| id | doaj.art-ae9b7aab18c147dc848a8e2e7c9b6de2 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T00:56:01Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ae9b7aab18c147dc848a8e2e7c9b6de22023-11-23T14:43:57ZengMDPI AGAntibiotics2079-63822022-08-01119115510.3390/antibiotics11091155Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored PlantHanan Y. Aati0Mariyam Anwar1Jawaher Al-Qahtani2Areej Al-Taweel3Kashif-ur-Rehman Khan4Sultan Aati5Faisal Usman6Bilal Ahmad Ghalloo7Hafiz Muhammad Asif8Jafir Hussain Shirazi9Aliza Abbasi10Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11495, Saudi ArabiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11495, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11495, Saudi ArabiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanUWA, University of Western Australia, Nedland, WA 6009, AustraliaDepartment of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60000, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanFaculty of Medicine and Allied Health Sciences, University College of Conventional Medicine, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan<i>Ficus vasta</i> Forssk. (Moraceae family) is an important medicinal plant that has not been previously investigated for its phytochemical and biological potential. Phytochemical screening, total bioactive content, and GCMS analysis were used to determine its phytoconstituents profile. Antioxidant, antibacterial, antifungal, anti-viral, cytotoxicity, thrombolytic, and enzyme inhibition activities were examined for biological evaluation. The plant extract exhibited the maximum total phenolic (89.47 ± 3.21 mg GAE/g) and total flavonoid contents (129.2 ± 4.14 mg QE/g), which may be related to the higher antioxidant potential of the extract. The extract showed strong α-amylase (IC<sub>50</sub> 5 ± 0.21 µg/mL) and α-glucosidase inhibition activity (IC<sub>50</sub> 5 ± 0.32 µg/mL). Significant results were observed in the case of antibacterial, antifungal, and anti-viral activities. The <i>F. vasta</i> extract inhibited the growth of HepG2 cells in a dose-dependent manner. The GCMS analysis of the extract provided the preliminary identification of 28 phytocompounds. In addition, the compounds identified by GCMS were subjected to in silico molecular docking analysis in order to identify any interactions between the compounds and enzymes (α-amylase and α-glucosidase). After that, the best-docked compounds were subjected to ADMET studies which provide information on pharmacokinetics, drug-likeness, physicochemical properties, and toxicity. The present study highlighted that the ethanol extract of <i>F. vasta</i> has antidiabetic, antimicrobial, anti-viral, and anti-cancer potentials that can be further explored for novel drug development.https://www.mdpi.com/2079-6382/11/9/1155<i>Ficus vasta</i>antibacterialanti-viralantifungalthrombolyticHepG2 cell line |
| spellingShingle | Hanan Y. Aati Mariyam Anwar Jawaher Al-Qahtani Areej Al-Taweel Kashif-ur-Rehman Khan Sultan Aati Faisal Usman Bilal Ahmad Ghalloo Hafiz Muhammad Asif Jafir Hussain Shirazi Aliza Abbasi Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored Plant Antibiotics <i>Ficus vasta</i> antibacterial anti-viral antifungal thrombolytic HepG2 cell line |
| title | Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored Plant |
| title_full | Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored Plant |
| title_fullStr | Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored Plant |
| title_full_unstemmed | Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored Plant |
| title_short | Phytochemical Profiling, In Vitro Biological Activities, and In-Silico Studies of <i>Ficus vasta Forssk.</i>: An Unexplored Plant |
| title_sort | phytochemical profiling in vitro biological activities and in silico studies of i ficus vasta forssk i an unexplored plant |
| topic | <i>Ficus vasta</i> antibacterial anti-viral antifungal thrombolytic HepG2 cell line |
| url | https://www.mdpi.com/2079-6382/11/9/1155 |
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