Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Complex
Carbapenem-resistant <i>Enterobacterales</i> (CRE) species are top priority pathogens according to the World Health Organization. Rapid detection is necessary and useful for their surveillance and control globally. This study developed a multiplex polymerase chain reaction (mPCR) detecti...
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MDPI AG
2022-12-01
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author | Rujirat Hatrongjit Peechanika Chopjitt Parichart Boueroy Anusak Kerdsin |
author_facet | Rujirat Hatrongjit Peechanika Chopjitt Parichart Boueroy Anusak Kerdsin |
author_sort | Rujirat Hatrongjit |
collection | DOAJ |
description | Carbapenem-resistant <i>Enterobacterales</i> (CRE) species are top priority pathogens according to the World Health Organization. Rapid detection is necessary and useful for their surveillance and control globally. This study developed a multiplex polymerase chain reaction (mPCR) detection of the common carbapenemase genes NDM, KPC, and OXA-48-like, together with identification of <i>Escherichia coli,</i> and distinguished a <i>Klebsiella pneumoniae</i> complex to be <i>K. pneumoniae</i>, <i>K. quasipneumoniae</i>, and <i>K. variicola</i>. Of 840 target <i>Enterobacterales</i> species, 190 <i>E. coli</i>, 598 <i>K. pneumoniae</i>, 28 <i>K. quasipneumoniae</i>, and 23 <i>K. variicola</i>. with and without NDM, KPC, or OXA-48-like were correctly detected for their species and carbapenemase genes. In contrast, for the <i>Enterobacterales</i> species other than <i>E. coli</i> or <i>K. pneumoniae</i> complex with carbapenemase genes, the mPCR assay could detect only NDM, KPC, or OXA-48-like. This PCR method should be useful in clinical microbiology laboratories requiring rapid detection of CRE for epidemiological investigation and for tracking the trends of carbapenemase gene dynamics. |
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language | English |
last_indexed | 2024-03-09T13:48:23Z |
publishDate | 2022-12-01 |
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series | Antibiotics |
spelling | doaj.art-ae9c52eb151c4804bbe129058e6f392f2023-11-30T20:55:22ZengMDPI AGAntibiotics2079-63822022-12-011217610.3390/antibiotics12010076Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> ComplexRujirat Hatrongjit0Peechanika Chopjitt1Parichart Boueroy2Anusak Kerdsin3Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandCarbapenem-resistant <i>Enterobacterales</i> (CRE) species are top priority pathogens according to the World Health Organization. Rapid detection is necessary and useful for their surveillance and control globally. This study developed a multiplex polymerase chain reaction (mPCR) detection of the common carbapenemase genes NDM, KPC, and OXA-48-like, together with identification of <i>Escherichia coli,</i> and distinguished a <i>Klebsiella pneumoniae</i> complex to be <i>K. pneumoniae</i>, <i>K. quasipneumoniae</i>, and <i>K. variicola</i>. Of 840 target <i>Enterobacterales</i> species, 190 <i>E. coli</i>, 598 <i>K. pneumoniae</i>, 28 <i>K. quasipneumoniae</i>, and 23 <i>K. variicola</i>. with and without NDM, KPC, or OXA-48-like were correctly detected for their species and carbapenemase genes. In contrast, for the <i>Enterobacterales</i> species other than <i>E. coli</i> or <i>K. pneumoniae</i> complex with carbapenemase genes, the mPCR assay could detect only NDM, KPC, or OXA-48-like. This PCR method should be useful in clinical microbiology laboratories requiring rapid detection of CRE for epidemiological investigation and for tracking the trends of carbapenemase gene dynamics.https://www.mdpi.com/2079-6382/12/1/76PCRcarbapenemaseNDMKPCOXA-48-like<i>Escherichia coli</i> |
spellingShingle | Rujirat Hatrongjit Peechanika Chopjitt Parichart Boueroy Anusak Kerdsin Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Complex Antibiotics PCR carbapenemase NDM KPC OXA-48-like <i>Escherichia coli</i> |
title | Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Complex |
title_full | Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Complex |
title_fullStr | Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Complex |
title_full_unstemmed | Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Complex |
title_short | Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically Relevant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Complex |
title_sort | multiplex pcr detection of common carbapenemase genes and identification of clinically relevant i escherichia coli i and i klebsiella pneumoniae i complex |
topic | PCR carbapenemase NDM KPC OXA-48-like <i>Escherichia coli</i> |
url | https://www.mdpi.com/2079-6382/12/1/76 |
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