CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer
Abstract The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and archaea. Various studies have shown that it is possible to target a wide range of human genes and treat some human diseases, including cancers, by the CRISPR/Cas9 system. In fact, CRISPR/Cas9 gene editing is one o...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | Cellular & Molecular Biology Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s11658-022-00348-2 |
| _version_ | 1811242232422858752 |
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| author | Mostafa Vaghari-Tabari Parisa Hassanpour Fatemeh Sadeghsoltani Faezeh Malakoti Forough Alemi Durdi Qujeq Zatollah Asemi Bahman Yousefi |
| author_facet | Mostafa Vaghari-Tabari Parisa Hassanpour Fatemeh Sadeghsoltani Faezeh Malakoti Forough Alemi Durdi Qujeq Zatollah Asemi Bahman Yousefi |
| author_sort | Mostafa Vaghari-Tabari |
| collection | DOAJ |
| description | Abstract The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and archaea. Various studies have shown that it is possible to target a wide range of human genes and treat some human diseases, including cancers, by the CRISPR/Cas9 system. In fact, CRISPR/Cas9 gene editing is one of the most efficient genome manipulation techniques. Studies have shown that CRISPR/Cas9 technology, in addition to having the potential to be used as a new therapeutic approach in the treatment of cancers, can also be used to enhance the effectiveness of existing treatments. Undoubtedly, the issue of drug resistance is one of the main obstacles in the treatment of cancers. Cancer cells resist anticancer drugs by a variety of mechanisms, such as enhancing anticancer drugs efflux, enhancing DNA repair, enhancing stemness, and attenuating apoptosis. Mutations in some proteins of different cellular signaling pathways are associated with these events and drug resistance. Recent studies have shown that the CRISPR/Cas9 technique can be used to target important genes involved in these mechanisms, thereby increasing the effectiveness of anticancer drugs. In this review article, studies related to the applications of this technique in overcoming drug resistance in cancer cells will be reviewed. In addition, we will give a brief overview of the limitations of the CRISP/Cas9 gene-editing technique. |
| first_indexed | 2024-04-12T13:47:40Z |
| format | Article |
| id | doaj.art-ae9c7458709c4d66a2da901077deec4b |
| institution | Directory Open Access Journal |
| issn | 1425-8153 1689-1392 |
| language | English |
| last_indexed | 2024-04-12T13:47:40Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cellular & Molecular Biology Letters |
| spelling | doaj.art-ae9c7458709c4d66a2da901077deec4b2022-12-22T03:30:37ZengBMCCellular & Molecular Biology Letters1425-81531689-13922022-06-0127112910.1186/s11658-022-00348-2CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancerMostafa Vaghari-Tabari0Parisa Hassanpour1Fatemeh Sadeghsoltani2Faezeh Malakoti3Forough Alemi4Durdi Qujeq5Zatollah Asemi6Bahman Yousefi7Department of Clinical Biochemistry and Laboratory Medicine, School of Medicine, Tabriz University of Medical SciencesDepartment of Clinical Biochemistry and Laboratory Medicine, School of Medicine, Tabriz University of Medical SciencesDepartment of Clinical Biochemistry and Laboratory Medicine, School of Medicine, Tabriz University of Medical SciencesDepartment of Clinical Biochemistry and Laboratory Medicine, School of Medicine, Tabriz University of Medical SciencesDepartment of Clinical Biochemistry and Laboratory Medicine, School of Medicine, Tabriz University of Medical SciencesCellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical SciencesResearch Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical SciencesDepartment of Clinical Biochemistry and Laboratory Medicine, School of Medicine, Tabriz University of Medical SciencesAbstract The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and archaea. Various studies have shown that it is possible to target a wide range of human genes and treat some human diseases, including cancers, by the CRISPR/Cas9 system. In fact, CRISPR/Cas9 gene editing is one of the most efficient genome manipulation techniques. Studies have shown that CRISPR/Cas9 technology, in addition to having the potential to be used as a new therapeutic approach in the treatment of cancers, can also be used to enhance the effectiveness of existing treatments. Undoubtedly, the issue of drug resistance is one of the main obstacles in the treatment of cancers. Cancer cells resist anticancer drugs by a variety of mechanisms, such as enhancing anticancer drugs efflux, enhancing DNA repair, enhancing stemness, and attenuating apoptosis. Mutations in some proteins of different cellular signaling pathways are associated with these events and drug resistance. Recent studies have shown that the CRISPR/Cas9 technique can be used to target important genes involved in these mechanisms, thereby increasing the effectiveness of anticancer drugs. In this review article, studies related to the applications of this technique in overcoming drug resistance in cancer cells will be reviewed. In addition, we will give a brief overview of the limitations of the CRISP/Cas9 gene-editing technique.https://doi.org/10.1186/s11658-022-00348-2CRISPR/Cas9Gene editingChemoresistanceMalignancyCancer treatment |
| spellingShingle | Mostafa Vaghari-Tabari Parisa Hassanpour Fatemeh Sadeghsoltani Faezeh Malakoti Forough Alemi Durdi Qujeq Zatollah Asemi Bahman Yousefi CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer Cellular & Molecular Biology Letters CRISPR/Cas9 Gene editing Chemoresistance Malignancy Cancer treatment |
| title | CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer |
| title_full | CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer |
| title_fullStr | CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer |
| title_full_unstemmed | CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer |
| title_short | CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer |
| title_sort | crispr cas9 gene editing a new approach for overcoming drug resistance in cancer |
| topic | CRISPR/Cas9 Gene editing Chemoresistance Malignancy Cancer treatment |
| url | https://doi.org/10.1186/s11658-022-00348-2 |
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