Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma
The prognosis of patients with metastatic lung adenocarcinoma (LUAD) is poor. Although novel lung cancer treatments have been developed for metastatic LUAD, not all patients are fit to receive these treatments. The present study aimed to identify the novel regulatory genes in metastatic LUAD. Becaus...
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MDPI AG
2021-04-01
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| author | Meng-Chi Yen Kuan-Li Wu Yu-Wei Liu Yung-Yun Chang Chao-Yuan Chang Jen-Yu Hung Ying-Ming Tsai Ya-Ling Hsu |
| author_facet | Meng-Chi Yen Kuan-Li Wu Yu-Wei Liu Yung-Yun Chang Chao-Yuan Chang Jen-Yu Hung Ying-Ming Tsai Ya-Ling Hsu |
| author_sort | Meng-Chi Yen |
| collection | DOAJ |
| description | The prognosis of patients with metastatic lung adenocarcinoma (LUAD) is poor. Although novel lung cancer treatments have been developed for metastatic LUAD, not all patients are fit to receive these treatments. The present study aimed to identify the novel regulatory genes in metastatic LUAD. Because the pleural cavity is a frequent metastasis site of LUAD, the adjacent non-tumor tissue, primary tumor tissue, and metastatic lung tumor tissue in the pleura of a single patient with LUAD were collected. The gene expression profiles of the collected samples were further analyzed via RNA sequencing and bioinformatic analysis. A high expression level of ubiquitin conjugating enzyme E2 H (UBE2H), a hypoxia-mediated gene, was identified in the metastatic malignant pleural tumor. After accessing the survival data in patients with lung adenocarcinoma through online databases, a high UBE2H expression was associated with poor survival for LUAD. UBE2H knockdown in two lung adenocarcinoma cell lines suppressed the cell migration capacity and reversed the epithelial–mesenchymal transition (EMT) signaling pathway. A high expression of UBE2H-targeting microRNAs, including miR-101, miR-30a, miR-30b, miR-328, and miR-497, were associated with a favorable prognosis. Moreover, the UBE2H expression revealed a significant correlation with the copy number variation. Taken together, the presence of UBE2H regulated the EMT program and metastasis in LUAD. |
| first_indexed | 2024-03-10T11:53:19Z |
| format | Article |
| id | doaj.art-ae9e193bb4ff4168a98caed8999bc47f |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T11:53:19Z |
| publishDate | 2021-04-01 |
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| series | Biology |
| spelling | doaj.art-ae9e193bb4ff4168a98caed8999bc47f2023-11-21T17:30:21ZengMDPI AGBiology2079-77372021-04-0110537810.3390/biology10050378Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung AdenocarcinomaMeng-Chi Yen0Kuan-Li Wu1Yu-Wei Liu2Yung-Yun Chang3Chao-Yuan Chang4Jen-Yu Hung5Ying-Ming Tsai6Ya-Ling Hsu7Department of Emergency Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanDivision of Thoracic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanDivision of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanDivision of Pulmonary and Critical Care Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanThe prognosis of patients with metastatic lung adenocarcinoma (LUAD) is poor. Although novel lung cancer treatments have been developed for metastatic LUAD, not all patients are fit to receive these treatments. The present study aimed to identify the novel regulatory genes in metastatic LUAD. Because the pleural cavity is a frequent metastasis site of LUAD, the adjacent non-tumor tissue, primary tumor tissue, and metastatic lung tumor tissue in the pleura of a single patient with LUAD were collected. The gene expression profiles of the collected samples were further analyzed via RNA sequencing and bioinformatic analysis. A high expression level of ubiquitin conjugating enzyme E2 H (UBE2H), a hypoxia-mediated gene, was identified in the metastatic malignant pleural tumor. After accessing the survival data in patients with lung adenocarcinoma through online databases, a high UBE2H expression was associated with poor survival for LUAD. UBE2H knockdown in two lung adenocarcinoma cell lines suppressed the cell migration capacity and reversed the epithelial–mesenchymal transition (EMT) signaling pathway. A high expression of UBE2H-targeting microRNAs, including miR-101, miR-30a, miR-30b, miR-328, and miR-497, were associated with a favorable prognosis. Moreover, the UBE2H expression revealed a significant correlation with the copy number variation. Taken together, the presence of UBE2H regulated the EMT program and metastasis in LUAD.https://www.mdpi.com/2079-7737/10/5/378lung adenocarcinomametastasishypoxiaubiquitin conjugating enzyme E2 H (UBE2H)RNA sequencingmicroRNA |
| spellingShingle | Meng-Chi Yen Kuan-Li Wu Yu-Wei Liu Yung-Yun Chang Chao-Yuan Chang Jen-Yu Hung Ying-Ming Tsai Ya-Ling Hsu Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma Biology lung adenocarcinoma metastasis hypoxia ubiquitin conjugating enzyme E2 H (UBE2H) RNA sequencing microRNA |
| title | Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma |
| title_full | Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma |
| title_fullStr | Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma |
| title_full_unstemmed | Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma |
| title_short | Ubiquitin Conjugating Enzyme E2 H (UBE2H) Is Linked to Poor Outcomes and Metastasis in Lung Adenocarcinoma |
| title_sort | ubiquitin conjugating enzyme e2 h ube2h is linked to poor outcomes and metastasis in lung adenocarcinoma |
| topic | lung adenocarcinoma metastasis hypoxia ubiquitin conjugating enzyme E2 H (UBE2H) RNA sequencing microRNA |
| url | https://www.mdpi.com/2079-7737/10/5/378 |
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