Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i>
The archaeon <i>Cuniculiplasma divulgatum</i> is ubiquitous in acidic environments with low-to-moderate temperatures. However, molecular mechanisms underlying its ability to thrive at lower temperatures remain unexplored. Using mass spectrometry (MS)-based proteomics, we analysed the eff...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/5/759 |
| _version_ | 1827716613062787072 |
|---|---|
| author | Rafael Bargiela Karin Lanthaler Colin M. Potter Manuel Ferrer Alexander F. Yakunin Bela Paizs Peter N. Golyshin Olga V. Golyshina |
| author_facet | Rafael Bargiela Karin Lanthaler Colin M. Potter Manuel Ferrer Alexander F. Yakunin Bela Paizs Peter N. Golyshin Olga V. Golyshina |
| author_sort | Rafael Bargiela |
| collection | DOAJ |
| description | The archaeon <i>Cuniculiplasma divulgatum</i> is ubiquitous in acidic environments with low-to-moderate temperatures. However, molecular mechanisms underlying its ability to thrive at lower temperatures remain unexplored. Using mass spectrometry (MS)-based proteomics, we analysed the effect of short-term (3 h) exposure to cold. The <i>C. divulgatum</i> genome encodes 2016 protein-coding genes, from which 819 proteins were identified in the cells grown under optimal conditions. In line with the peptidolytic lifestyle of <i>C. divulgatum</i>, its intracellular proteome revealed the abundance of proteases, ABC transporters and cytochrome C oxidase. From 747 quantifiable polypeptides, the levels of 582 proteins showed no change after the cold shock, whereas 104 proteins were upregulated suggesting that they might be contributing to cold adaptation. The highest increase in expression appeared in low-abundance (0.001–0.005 fmol%) proteins for polypeptides’ hydrolysis (metal-dependent hydrolase), oxidation of amino acids (FAD-dependent oxidoreductase), pyrimidine biosynthesis (aspartate carbamoyltransferase regulatory chain proteins), citrate cycle (2-oxoacid ferredoxin oxidoreductase) and ATP production (V type ATP synthase). Importantly, the cold shock induced a substantial increase (6% and 9%) in expression of the most-abundant proteins, thermosome beta subunit and glutamate dehydrogenase. This study has outlined potential mechanisms of environmental fitness of <i>Cuniculiplasma</i> spp. allowing them to colonise acidic settings at low/moderate temperatures. |
| first_indexed | 2024-03-10T19:45:03Z |
| format | Article |
| id | doaj.art-ae9e1a4e4b08496bbe5f5e17a5be0d99 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:45:03Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ae9e1a4e4b08496bbe5f5e17a5be0d992023-11-20T00:55:53ZengMDPI AGMicroorganisms2076-26072020-05-018575910.3390/microorganisms8050759Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i>Rafael Bargiela0Karin Lanthaler1Colin M. Potter2Manuel Ferrer3Alexander F. Yakunin4Bela Paizs5Peter N. Golyshin6Olga V. Golyshina7School of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UKSchool of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UKSchool of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UKSystems Biotechnology Group, Department of Applied Biocatalysis, CSIC—Institute of Catalysis, Marie Curie 2, 28049 Madrid, SpainSchool of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UKSchool of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UKSchool of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UKSchool of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UKThe archaeon <i>Cuniculiplasma divulgatum</i> is ubiquitous in acidic environments with low-to-moderate temperatures. However, molecular mechanisms underlying its ability to thrive at lower temperatures remain unexplored. Using mass spectrometry (MS)-based proteomics, we analysed the effect of short-term (3 h) exposure to cold. The <i>C. divulgatum</i> genome encodes 2016 protein-coding genes, from which 819 proteins were identified in the cells grown under optimal conditions. In line with the peptidolytic lifestyle of <i>C. divulgatum</i>, its intracellular proteome revealed the abundance of proteases, ABC transporters and cytochrome C oxidase. From 747 quantifiable polypeptides, the levels of 582 proteins showed no change after the cold shock, whereas 104 proteins were upregulated suggesting that they might be contributing to cold adaptation. The highest increase in expression appeared in low-abundance (0.001–0.005 fmol%) proteins for polypeptides’ hydrolysis (metal-dependent hydrolase), oxidation of amino acids (FAD-dependent oxidoreductase), pyrimidine biosynthesis (aspartate carbamoyltransferase regulatory chain proteins), citrate cycle (2-oxoacid ferredoxin oxidoreductase) and ATP production (V type ATP synthase). Importantly, the cold shock induced a substantial increase (6% and 9%) in expression of the most-abundant proteins, thermosome beta subunit and glutamate dehydrogenase. This study has outlined potential mechanisms of environmental fitness of <i>Cuniculiplasma</i> spp. allowing them to colonise acidic settings at low/moderate temperatures.https://www.mdpi.com/2076-2607/8/5/759Acidophilic archaeaacidic environmentsacid mine drainage systems<i>Thermoplasmatales</i><i>Cuniculiplasma</i>proteome |
| spellingShingle | Rafael Bargiela Karin Lanthaler Colin M. Potter Manuel Ferrer Alexander F. Yakunin Bela Paizs Peter N. Golyshin Olga V. Golyshina Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i> Microorganisms Acidophilic archaea acidic environments acid mine drainage systems <i>Thermoplasmatales</i> <i>Cuniculiplasma</i> proteome |
| title | Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i> |
| title_full | Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i> |
| title_fullStr | Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i> |
| title_full_unstemmed | Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i> |
| title_short | Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, <i>Cuniculiplasma divulgatum</i> |
| title_sort | proteome cold shock response in the extremely acidophilic archaeon i cuniculiplasma divulgatum i |
| topic | Acidophilic archaea acidic environments acid mine drainage systems <i>Thermoplasmatales</i> <i>Cuniculiplasma</i> proteome |
| url | https://www.mdpi.com/2076-2607/8/5/759 |
| work_keys_str_mv | AT rafaelbargiela proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi AT karinlanthaler proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi AT colinmpotter proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi AT manuelferrer proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi AT alexanderfyakunin proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi AT belapaizs proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi AT peterngolyshin proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi AT olgavgolyshina proteomecoldshockresponseintheextremelyacidophilicarchaeonicuniculiplasmadivulgatumi |