Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeA
Rice (<i>Oryzae sativa</i> cv. dongjin) is a cornerstone of global food security; however, <i>Burkholderia glumae</i> BGR1, which is responsible for bacterial panicle blight (BPB), threatens its productive output, with dire consequences for rice and other crops. BPB is primar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/23/3934 |
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| author | Namgyu Kim Duyoung Lee Sais-Beul Lee Gah-Hyun Lim Sang-Woo Kim Tae-Jin Kim Dong-Soo Park Young-Su Seo |
| author_facet | Namgyu Kim Duyoung Lee Sais-Beul Lee Gah-Hyun Lim Sang-Woo Kim Tae-Jin Kim Dong-Soo Park Young-Su Seo |
| author_sort | Namgyu Kim |
| collection | DOAJ |
| description | Rice (<i>Oryzae sativa</i> cv. dongjin) is a cornerstone of global food security; however, <i>Burkholderia glumae</i> BGR1, which is responsible for bacterial panicle blight (BPB), threatens its productive output, with dire consequences for rice and other crops. BPB is primarily caused by toxoflavin, a potent phytotoxin that disrupts plant growth at various developmental stages. Therefore, understanding the mechanisms through which toxoflavin and BPB affect rice plants is critical. Toxoflavin biosynthesis in <i>B. glumae</i> BGR1 relies on the <i>toxABCDE</i> operon, with ToxA playing a central role. In response to this threat, our study explores a metagenome-derived toxoflavin-degrading enzyme, TxeA, as a potential defense mechanism against toxoflavin’s destructive impact. TxeA-induced degradation of toxoflavin represents a potential strategy to mitigate crop damage. We introduce a groundbreaking approach: engineering transgenic rice plants to produce toxoflavin-degrading enzymes. These genetically modified plants, armed with TxeA, hold significant potential for combating toxoflavin-related crop losses. However, removal of toxoflavin, a major virulence factor in <i>B. glumae</i> BGR1, does not completely inhibit virulence. This innovative perspective offers a new shift from pathogen eradication to leveraging transgenic plants’ power, offering a beacon of hope for crop protection and disease management. Our study offers insights into the intricate interplay between toxoflavin, BPB, and TxeA, providing a promising avenue to safeguard rice crops, ensure food security, and potentially enhance the resilience of various agricultural crops to <i>B. glumae</i> BGR1-induced diseases. |
| first_indexed | 2024-03-09T01:45:14Z |
| format | Article |
| id | doaj.art-ae9f139c558d45c49cde19a9f6269d16 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T01:45:14Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ae9f139c558d45c49cde19a9f6269d162023-12-08T15:23:41ZengMDPI AGPlants2223-77472023-11-011223393410.3390/plants12233934Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeANamgyu Kim0Duyoung Lee1Sais-Beul Lee2Gah-Hyun Lim3Sang-Woo Kim4Tae-Jin Kim5Dong-Soo Park6Young-Su Seo7Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, Republic of KoreaNational Institute of Crop Science, Milyang 50424, Republic of KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, Republic of KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, Republic of KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, Republic of KoreaNational Institute of Crop Science, Milyang 50424, Republic of KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, Republic of KoreaRice (<i>Oryzae sativa</i> cv. dongjin) is a cornerstone of global food security; however, <i>Burkholderia glumae</i> BGR1, which is responsible for bacterial panicle blight (BPB), threatens its productive output, with dire consequences for rice and other crops. BPB is primarily caused by toxoflavin, a potent phytotoxin that disrupts plant growth at various developmental stages. Therefore, understanding the mechanisms through which toxoflavin and BPB affect rice plants is critical. Toxoflavin biosynthesis in <i>B. glumae</i> BGR1 relies on the <i>toxABCDE</i> operon, with ToxA playing a central role. In response to this threat, our study explores a metagenome-derived toxoflavin-degrading enzyme, TxeA, as a potential defense mechanism against toxoflavin’s destructive impact. TxeA-induced degradation of toxoflavin represents a potential strategy to mitigate crop damage. We introduce a groundbreaking approach: engineering transgenic rice plants to produce toxoflavin-degrading enzymes. These genetically modified plants, armed with TxeA, hold significant potential for combating toxoflavin-related crop losses. However, removal of toxoflavin, a major virulence factor in <i>B. glumae</i> BGR1, does not completely inhibit virulence. This innovative perspective offers a new shift from pathogen eradication to leveraging transgenic plants’ power, offering a beacon of hope for crop protection and disease management. Our study offers insights into the intricate interplay between toxoflavin, BPB, and TxeA, providing a promising avenue to safeguard rice crops, ensure food security, and potentially enhance the resilience of various agricultural crops to <i>B. glumae</i> BGR1-induced diseases.https://www.mdpi.com/2223-7747/12/23/3934toxoflavintoxoflavin-degrading enzymeTxeA<i>Burkholderia glumae</i>transgenic plantsresistance |
| spellingShingle | Namgyu Kim Duyoung Lee Sais-Beul Lee Gah-Hyun Lim Sang-Woo Kim Tae-Jin Kim Dong-Soo Park Young-Su Seo Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeA Plants toxoflavin toxoflavin-degrading enzyme TxeA <i>Burkholderia glumae</i> transgenic plants resistance |
| title | Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeA |
| title_full | Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeA |
| title_fullStr | Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeA |
| title_full_unstemmed | Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeA |
| title_short | Understanding <i>Burkholderia glumae</i> BGR1 Virulence through the Application of Toxoflavin-Degrading Enzyme, TxeA |
| title_sort | understanding i burkholderia glumae i bgr1 virulence through the application of toxoflavin degrading enzyme txea |
| topic | toxoflavin toxoflavin-degrading enzyme TxeA <i>Burkholderia glumae</i> transgenic plants resistance |
| url | https://www.mdpi.com/2223-7747/12/23/3934 |
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