Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypes
Objective: The aim of the study was to explore the effect of semi-specific antagonists and agonists of the nicotinic acetylcholine receptors on the paired-pulse facilitation and nicotinic tonic and phasic potentiation of the frog retinotectal synaptic transmission. Materials and methods: The experim...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-01-01
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| Series: | Medicina |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1010660X15000269 |
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| author | Armuntas Baginskas Vilija Kuraitė Antanas Kuras |
| author_facet | Armuntas Baginskas Vilija Kuraitė Antanas Kuras |
| author_sort | Armuntas Baginskas |
| collection | DOAJ |
| description | Objective: The aim of the study was to explore the effect of semi-specific antagonists and agonists of the nicotinic acetylcholine receptors on the paired-pulse facilitation and nicotinic tonic and phasic potentiation of the frog retinotectal synaptic transmission.
Materials and methods: The experiments were performed in vivo on adult frogs, Rana temporaria. An individual retina ganglion cell (or its retinotectal fiber) was stimulated by current pulses delivered through multichannel stimulating electrode positioned on the retina. Responses to a discharge of a single retinal ganglion cell were recorded in the tectum by an extracellular carbon-fiber microelectrode positioned in the terminal arborization of the retinotectal fiber in the tectum layer F. The effect of the antagonists and agonists of the nicotinic acetylcholine receptors on the tectal responses has been tested.
Results: We found that the antagonists, MLA and DHβE, and agonists, RJR-2403 and choline, of the nicotinic acetylcholine receptors of the α3β2, α4β2, α2β4, α6β2 or α7 subtypes have had no effect on the phasic and tonic potentiation of the retinotectal transmission. The paired-pulse facilitation of the retinotectal transmission was not appreciably affected by the antagonists, but the choline, agonist of the α7 subtype receptor, has significantly decreased the paired-pulse facilitation.
Conclusions: The tonic and phasic potentiation of the retinotectal transmission in the tectum layer F were not mediated by the receptors of α3β2, α4β2, α2β4, α6β2 or α7 subtype. The results suggest that presynaptic nicotinic acetylcholine receptors of the frog optic fibers are different from those of the mammalian optic fibers. |
| first_indexed | 2024-03-12T09:07:08Z |
| format | Article |
| id | doaj.art-aea1ab2a8f004cd99442178e1e48ca6e |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T09:07:08Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-aea1ab2a8f004cd99442178e1e48ca6e2023-09-02T15:16:13ZengMDPI AGMedicina1010-660X2015-01-0151211712510.1016/j.medici.2015.03.002Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypesArmuntas Baginskas0Vilija Kuraitė1Antanas Kuras2Department of Physics, Mathematics and Biophysics, Medical Academy, Lithuanian University of Health Sciences, Kaunas, LithuaniaLaboratory of Neurophysiology, Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Kaunas, LithuaniaLaboratory of Neurophysiology, Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Kaunas, LithuaniaObjective: The aim of the study was to explore the effect of semi-specific antagonists and agonists of the nicotinic acetylcholine receptors on the paired-pulse facilitation and nicotinic tonic and phasic potentiation of the frog retinotectal synaptic transmission. Materials and methods: The experiments were performed in vivo on adult frogs, Rana temporaria. An individual retina ganglion cell (or its retinotectal fiber) was stimulated by current pulses delivered through multichannel stimulating electrode positioned on the retina. Responses to a discharge of a single retinal ganglion cell were recorded in the tectum by an extracellular carbon-fiber microelectrode positioned in the terminal arborization of the retinotectal fiber in the tectum layer F. The effect of the antagonists and agonists of the nicotinic acetylcholine receptors on the tectal responses has been tested. Results: We found that the antagonists, MLA and DHβE, and agonists, RJR-2403 and choline, of the nicotinic acetylcholine receptors of the α3β2, α4β2, α2β4, α6β2 or α7 subtypes have had no effect on the phasic and tonic potentiation of the retinotectal transmission. The paired-pulse facilitation of the retinotectal transmission was not appreciably affected by the antagonists, but the choline, agonist of the α7 subtype receptor, has significantly decreased the paired-pulse facilitation. Conclusions: The tonic and phasic potentiation of the retinotectal transmission in the tectum layer F were not mediated by the receptors of α3β2, α4β2, α2β4, α6β2 or α7 subtype. The results suggest that presynaptic nicotinic acetylcholine receptors of the frog optic fibers are different from those of the mammalian optic fibers.http://www.sciencedirect.com/science/article/pii/S1010660X15000269Optic fiberFrog tectumRetinotectal synaptic transmissionPresynaptic nicotinic acetylcholine receptorsNicotinic potentiation |
| spellingShingle | Armuntas Baginskas Vilija Kuraitė Antanas Kuras Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypes Medicina Optic fiber Frog tectum Retinotectal synaptic transmission Presynaptic nicotinic acetylcholine receptors Nicotinic potentiation |
| title | Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypes |
| title_full | Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypes |
| title_fullStr | Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypes |
| title_full_unstemmed | Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypes |
| title_short | Nicotinic potentiation of frog retinotectal transmission in tectum layer F by α3β2, α4β2, α2β4, α6β2, or α7 acetylcholine receptor subtypes |
| title_sort | nicotinic potentiation of frog retinotectal transmission in tectum layer f by α3β2 α4β2 α2β4 α6β2 or α7 acetylcholine receptor subtypes |
| topic | Optic fiber Frog tectum Retinotectal synaptic transmission Presynaptic nicotinic acetylcholine receptors Nicotinic potentiation |
| url | http://www.sciencedirect.com/science/article/pii/S1010660X15000269 |
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