Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article)
Background: Pneumocystis jirovecii (P. jirovecii) causes Pneumocystis pneumonia (PCP) in people, especially the immunocompromised ones. It is also one of the serious causes of numerous lung problems in affected patients. Since documented data about P. jirovecii is not available in patients with pulm...
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| Format: | Article |
| Language: | fas |
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Ardabil University of Medical Sciences
2023-12-01
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| Series: | Journal of Ardabil University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jarums.arums.ac.ir/article-1-2262-en.pdf |
| _version_ | 1797205862099451904 |
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| author | Narges Chitsaz Ahmad Reza Meamar Elham Razmjou Soheila Shafaghi-Sisi Maryam Alipour Maryam Sadeghi Zahra Rampisheh Zeinab Ghasemi Rasoul Aliannejad Alireza Badirzadeh |
| author_facet | Narges Chitsaz Ahmad Reza Meamar Elham Razmjou Soheila Shafaghi-Sisi Maryam Alipour Maryam Sadeghi Zahra Rampisheh Zeinab Ghasemi Rasoul Aliannejad Alireza Badirzadeh |
| author_sort | Narges Chitsaz |
| collection | DOAJ |
| description | Background: Pneumocystis jirovecii (P. jirovecii) causes Pneumocystis pneumonia (PCP) in people, especially the immunocompromised ones. It is also one of the serious causes of numerous lung problems in affected patients. Since documented data about P. jirovecii is not available in patients with pulmonary infections in Tehran, this study aimed to investigate the molecular epidemiology and parasitology of Pneumocystis to determine the frequency of the organism infection.
Methods: Bronchoalveolar lavage (BAL) samples were collected for 367 patients hospitalized in the lung department of Shariati Hospital in Tehran from July 2022 to July 2023. The samples were analyzed using Giemsa staining and molecular methods. After DNA extraction from samples, Nested polymerase chain reaction (Nested PCR) was employed for the amplification of the 18SrRNA gene and identification of P. jirovecii. The PCR products of Nested PCR were sequenced for final confirmation.
Results: Out of 367 samples, only one sample (0.27%) and 28 samples (6.7%) were found to be positive through parasitology and NestedPCR analysis, respectively. P. jirovecii was detected in seven (25%) and 21 (75%) immunocompromised and immunocompetent patients, respectively. Fever, shortness of breath and dry cough were the most common clinical symptoms among patients with Pneumocystosis. Patients with pulmonary disorders are prone to colonization by pneumocystis, which increases the risk of pneumocystosis and makes them a reservoir for transmission to susceptible people.
Conclusion: It can be concluded that patients with distinct lung disease are prone to colonization by Pneumocystis and, importantly, are at risk of infection. Also, according to the current study, Nested PCR was a suitable method for detecting P. jirovecii organisms because it had a very high sensitivity and specificity. |
| first_indexed | 2024-04-24T08:57:51Z |
| format | Article |
| id | doaj.art-aea2e03201d64059832b49693ee6b4f8 |
| institution | Directory Open Access Journal |
| issn | 2228-7280 2228-7299 |
| language | fas |
| last_indexed | 2024-04-24T08:57:51Z |
| publishDate | 2023-12-01 |
| publisher | Ardabil University of Medical Sciences |
| record_format | Article |
| series | Journal of Ardabil University of Medical Sciences |
| spelling | doaj.art-aea2e03201d64059832b49693ee6b4f82024-04-16T07:05:13ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992023-12-01234330342Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article)Narges Chitsaz0Ahmad Reza Meamar1Elham Razmjou2Soheila Shafaghi-Sisi3Maryam Alipour4Maryam Sadeghi5Zahra Rampisheh6Zeinab Ghasemi7Rasoul Aliannejad8Alireza Badirzadeh9 Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Preventive Medicine and Public Health Research Center, Psychosocial Health Research Institute, Department of Community and Family Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Mycology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran Division of pulmonary and critical care, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Background: Pneumocystis jirovecii (P. jirovecii) causes Pneumocystis pneumonia (PCP) in people, especially the immunocompromised ones. It is also one of the serious causes of numerous lung problems in affected patients. Since documented data about P. jirovecii is not available in patients with pulmonary infections in Tehran, this study aimed to investigate the molecular epidemiology and parasitology of Pneumocystis to determine the frequency of the organism infection. Methods: Bronchoalveolar lavage (BAL) samples were collected for 367 patients hospitalized in the lung department of Shariati Hospital in Tehran from July 2022 to July 2023. The samples were analyzed using Giemsa staining and molecular methods. After DNA extraction from samples, Nested polymerase chain reaction (Nested PCR) was employed for the amplification of the 18SrRNA gene and identification of P. jirovecii. The PCR products of Nested PCR were sequenced for final confirmation. Results: Out of 367 samples, only one sample (0.27%) and 28 samples (6.7%) were found to be positive through parasitology and NestedPCR analysis, respectively. P. jirovecii was detected in seven (25%) and 21 (75%) immunocompromised and immunocompetent patients, respectively. Fever, shortness of breath and dry cough were the most common clinical symptoms among patients with Pneumocystosis. Patients with pulmonary disorders are prone to colonization by pneumocystis, which increases the risk of pneumocystosis and makes them a reservoir for transmission to susceptible people. Conclusion: It can be concluded that patients with distinct lung disease are prone to colonization by Pneumocystis and, importantly, are at risk of infection. Also, according to the current study, Nested PCR was a suitable method for detecting P. jirovecii organisms because it had a very high sensitivity and specificity.http://jarums.arums.ac.ir/article-1-2262-en.pdfpneumocystis jiroveciipneumoniacolonizationnested-pcrbroncho-alveolar lavage (bal) |
| spellingShingle | Narges Chitsaz Ahmad Reza Meamar Elham Razmjou Soheila Shafaghi-Sisi Maryam Alipour Maryam Sadeghi Zahra Rampisheh Zeinab Ghasemi Rasoul Aliannejad Alireza Badirzadeh Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article) Journal of Ardabil University of Medical Sciences pneumocystis jirovecii pneumonia colonization nested-pcr broncho-alveolar lavage (bal) |
| title | Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article) |
| title_full | Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article) |
| title_fullStr | Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article) |
| title_full_unstemmed | Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article) |
| title_short | Molecular Detection of Pneumocystis jirovecii in the Bronchoalveolar Lavage Samples in Patients with Various Pulmonary Disorders(Original Article) |
| title_sort | molecular detection of pneumocystis jirovecii in the bronchoalveolar lavage samples in patients with various pulmonary disorders original article |
| topic | pneumocystis jirovecii pneumonia colonization nested-pcr broncho-alveolar lavage (bal) |
| url | http://jarums.arums.ac.ir/article-1-2262-en.pdf |
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