Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells
Cerium oxide nanoparticles (nanoceria) are generally known for their recyclable antioxidative properties making them an appealing biomaterial for protecting against physiological and pathological age-related changes that are caused by reactive oxygen species (ROS). Cataract is one such pathology tha...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/6/1473 |
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| author | Belal I. Hanafy Gareth W. V. Cave Yvonne Barnett Barbara K. Pierscionek |
| author_facet | Belal I. Hanafy Gareth W. V. Cave Yvonne Barnett Barbara K. Pierscionek |
| author_sort | Belal I. Hanafy |
| collection | DOAJ |
| description | Cerium oxide nanoparticles (nanoceria) are generally known for their recyclable antioxidative properties making them an appealing biomaterial for protecting against physiological and pathological age-related changes that are caused by reactive oxygen species (ROS). Cataract is one such pathology that has been associated with oxidation and glycation of the lens proteins (crystallins) leading to aggregation and opacification. A novel coated nanoceria formulation has been previously shown to enter the human lens epithelial cells (HLECs) and protect them from oxidative stress induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). In this work, the mechanism of nanoceria uptake in HLECs is studied and multiple anti-cataractogenic properties are assessed in vitro. Our results show that the nanoceria provide multiple beneficial actions to delay cataract progression by (1) acting as a catalase mimetic in cells with inhibited catalase, (2) improving reduced to oxidised glutathione ratio (GSH/GSSG) in HLECs, and (3) inhibiting the non-enzymatic glucose-induced glycation of the chaperone lens protein α-crystallin. Given the multifactorial nature of cataract progression, the varied actions of nanoceria render them promising candidates for potential non-surgical therapeutic treatment. |
| first_indexed | 2024-03-10T10:48:01Z |
| format | Article |
| id | doaj.art-aea4b8159a4e4561b6fb0233fcc8619a |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T10:48:01Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-aea4b8159a4e4561b6fb0233fcc8619a2023-11-21T22:28:47ZengMDPI AGNanomaterials2079-49912021-06-01116147310.3390/nano11061473Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens CellsBelal I. Hanafy0Gareth W. V. Cave1Yvonne Barnett2Barbara K. Pierscionek3School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UKSchool of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UKFaculty of Heath, Education, Medicine and Social Care and Pharmaceutical Research Group, Medical Technology Research Centre, Anglia Ruskin University, Cambridgeshire CB1 1PT, UKFaculty of Heath, Education, Medicine and Social Care and Pharmaceutical Research Group, Medical Technology Research Centre, Anglia Ruskin University, Cambridgeshire CB1 1PT, UKCerium oxide nanoparticles (nanoceria) are generally known for their recyclable antioxidative properties making them an appealing biomaterial for protecting against physiological and pathological age-related changes that are caused by reactive oxygen species (ROS). Cataract is one such pathology that has been associated with oxidation and glycation of the lens proteins (crystallins) leading to aggregation and opacification. A novel coated nanoceria formulation has been previously shown to enter the human lens epithelial cells (HLECs) and protect them from oxidative stress induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). In this work, the mechanism of nanoceria uptake in HLECs is studied and multiple anti-cataractogenic properties are assessed in vitro. Our results show that the nanoceria provide multiple beneficial actions to delay cataract progression by (1) acting as a catalase mimetic in cells with inhibited catalase, (2) improving reduced to oxidised glutathione ratio (GSH/GSSG) in HLECs, and (3) inhibiting the non-enzymatic glucose-induced glycation of the chaperone lens protein α-crystallin. Given the multifactorial nature of cataract progression, the varied actions of nanoceria render them promising candidates for potential non-surgical therapeutic treatment.https://www.mdpi.com/2079-4991/11/6/1473cerium oxide nanoparticlesglycationcataractglutathioneendocytosis |
| spellingShingle | Belal I. Hanafy Gareth W. V. Cave Yvonne Barnett Barbara K. Pierscionek Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells Nanomaterials cerium oxide nanoparticles glycation cataract glutathione endocytosis |
| title | Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells |
| title_full | Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells |
| title_fullStr | Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells |
| title_full_unstemmed | Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells |
| title_short | Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells |
| title_sort | nanoceria prevents glucose induced protein glycation in eye lens cells |
| topic | cerium oxide nanoparticles glycation cataract glutathione endocytosis |
| url | https://www.mdpi.com/2079-4991/11/6/1473 |
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