Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model
The ambrosia beetle <i>Xyleborus affinis</i>, recently reported affecting avocado trees in Mexico, represents one of the most widespread insects worldwide. Previous reports have shown that <i>Xyleborus</i> genera members are susceptible to <i>Beauveria bassiana</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/5/477 |
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| author | Jesús E. Castrejón-Antonio Patricia Tamez-Guerra Nohemi García-Ortiz Facundo Muñiz-Paredes Juan Carlos Sánchez-Rangel Roberto Montesinos-Matías |
| author_facet | Jesús E. Castrejón-Antonio Patricia Tamez-Guerra Nohemi García-Ortiz Facundo Muñiz-Paredes Juan Carlos Sánchez-Rangel Roberto Montesinos-Matías |
| author_sort | Jesús E. Castrejón-Antonio |
| collection | DOAJ |
| description | The ambrosia beetle <i>Xyleborus affinis</i>, recently reported affecting avocado trees in Mexico, represents one of the most widespread insects worldwide. Previous reports have shown that <i>Xyleborus</i> genera members are susceptible to <i>Beauveria bassiana</i> and other entomopathogenic fungus strains. However, their effect on borer beetles’ progeny has not been fully investigated. The aim of the present study was to determine the insecticidal activity of <i>B. bassiana</i> on <i>X. affinis</i> adult females and their progeny in an artificial sawdust diet bioassay model. The <i>B. bassiana</i> strains CHE-CNRCB 44, 171, 431, and 485 were individually tested on females at concentrations ranging from 2 × 10<sup>6</sup> to 1 × 10<sup>9</sup> conidia mL<sup>−1</sup>. After 10 d of incubation, diet was evaluated to count laid eggs, larvae, and adults. Insect conidia loss after exposure was determined by attached conidia to each insect after 12 h of exposure. The results showed that females’ mortality ranged between 3.4% and 50.3% in a concentration–response manner. Furthermore, we did not observe statistical differences among strains at the highest concentration. CHE-CNRCB 44 showed the highest mortality at the lowest concentration and reduced larvae and laid eggs at the highest concentration (<i>p</i> < 0.01). Strains CHE-CNRCB 44, 431, and 485 significantly decreased larvae, as compared with the untreated control. After 12 h, up to 70% of conidia was removed by the effect of the artificial diet. In conclusion, <i>B. bassiana</i> has the potential to control <i>X. affinis</i> adult females and progeny. |
| first_indexed | 2024-03-11T03:37:47Z |
| format | Article |
| id | doaj.art-aeaba211d4b34f9c8162418c1c7d3aa8 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T03:37:47Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-aeaba211d4b34f9c8162418c1c7d3aa82023-11-18T01:50:34ZengMDPI AGInsects2075-44502023-05-0114547710.3390/insects14050477Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet ModelJesús E. Castrejón-Antonio0Patricia Tamez-Guerra1Nohemi García-Ortiz2Facundo Muñiz-Paredes3Juan Carlos Sánchez-Rangel4Roberto Montesinos-Matías5Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Autopista Colima-Manzanillo km 40, La Estación, Tecomán C.P. 28930, Colima, MexicoFacultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba S/N, Cd. Universitaria, San Nicolás de los Garza C.P. 66455, Nuevo León, MexicoCentro Nacional de Referencia de Control Biológico, km 1.5, Carretera Tecomán-Estación FFCC, Col. Tepeyac, Tecomán C.P. 28110, Colima, MexicoCentro Nacional de Referencia de Control Biológico, km 1.5, Carretera Tecomán-Estación FFCC, Col. Tepeyac, Tecomán C.P. 28110, Colima, MexicoFacultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Autopista Colima-Manzanillo km 40, La Estación, Tecomán C.P. 28930, Colima, MexicoCentro Nacional de Referencia de Control Biológico, km 1.5, Carretera Tecomán-Estación FFCC, Col. Tepeyac, Tecomán C.P. 28110, Colima, MexicoThe ambrosia beetle <i>Xyleborus affinis</i>, recently reported affecting avocado trees in Mexico, represents one of the most widespread insects worldwide. Previous reports have shown that <i>Xyleborus</i> genera members are susceptible to <i>Beauveria bassiana</i> and other entomopathogenic fungus strains. However, their effect on borer beetles’ progeny has not been fully investigated. The aim of the present study was to determine the insecticidal activity of <i>B. bassiana</i> on <i>X. affinis</i> adult females and their progeny in an artificial sawdust diet bioassay model. The <i>B. bassiana</i> strains CHE-CNRCB 44, 171, 431, and 485 were individually tested on females at concentrations ranging from 2 × 10<sup>6</sup> to 1 × 10<sup>9</sup> conidia mL<sup>−1</sup>. After 10 d of incubation, diet was evaluated to count laid eggs, larvae, and adults. Insect conidia loss after exposure was determined by attached conidia to each insect after 12 h of exposure. The results showed that females’ mortality ranged between 3.4% and 50.3% in a concentration–response manner. Furthermore, we did not observe statistical differences among strains at the highest concentration. CHE-CNRCB 44 showed the highest mortality at the lowest concentration and reduced larvae and laid eggs at the highest concentration (<i>p</i> < 0.01). Strains CHE-CNRCB 44, 431, and 485 significantly decreased larvae, as compared with the untreated control. After 12 h, up to 70% of conidia was removed by the effect of the artificial diet. In conclusion, <i>B. bassiana</i> has the potential to control <i>X. affinis</i> adult females and progeny.https://www.mdpi.com/2075-4450/14/5/477ambrosia beetlesentomopathogenic funguslaid eggssawdust dietconidial attachment |
| spellingShingle | Jesús E. Castrejón-Antonio Patricia Tamez-Guerra Nohemi García-Ortiz Facundo Muñiz-Paredes Juan Carlos Sánchez-Rangel Roberto Montesinos-Matías Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model Insects ambrosia beetles entomopathogenic fungus laid eggs sawdust diet conidial attachment |
| title | Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model |
| title_full | Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model |
| title_fullStr | Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model |
| title_full_unstemmed | Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model |
| title_short | Biocontrol of <i>Xyleborus affinis</i> (Curculionidae: Scolitinae) Females and Progeny by <i>Beauveria bassiana</i> (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model |
| title_sort | biocontrol of i xyleborus affinis i curculionidae scolitinae females and progeny by i beauveria bassiana i hypocreales cordycipitaceae in a sawdust artificial diet model |
| topic | ambrosia beetles entomopathogenic fungus laid eggs sawdust diet conidial attachment |
| url | https://www.mdpi.com/2075-4450/14/5/477 |
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