Prion pathogenesis in the absence of NLRP3/ASC inflammasomes.
The accumulation of the scrapie prion protein PrPSc, a misfolded conformer of the cellular prion protein PrPC, is a crucial feature of prion diseases. In the central nervous system, this process is accompanied by conspicuous microglia activation. The NLRP3 inflammasome is a multi-molecular complex w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4324825?pdf=render |
| _version_ | 1818013262079328256 |
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| author | Mario Nuvolone Silvia Sorce Petra Schwarz Adriano Aguzzi |
| author_facet | Mario Nuvolone Silvia Sorce Petra Schwarz Adriano Aguzzi |
| author_sort | Mario Nuvolone |
| collection | DOAJ |
| description | The accumulation of the scrapie prion protein PrPSc, a misfolded conformer of the cellular prion protein PrPC, is a crucial feature of prion diseases. In the central nervous system, this process is accompanied by conspicuous microglia activation. The NLRP3 inflammasome is a multi-molecular complex which can sense heterogeneous pathogen-associated molecular patterns and culminates in the activation of caspase 1 and release of IL 1β. The NLRP3 inflammasome was reported to be essential for IL 1β release after in vitro exposure to the amyloidogenic peptide PrP106-126 and to recombinant PrP fibrils. We therefore studied the role of the NLRP3 inflammasome in a mouse model of prion infection. Upon intracerebral inoculation with scrapie prions (strain RML), mice lacking NLRP3 (Nlrp3-/-) or the inflammasome adaptor protein ASC (Pycard-/-) succumbed to scrapie with attack rates and incubation times similar to wild-type mice, and developed the classic histologic and biochemical features of prion diseases. Genetic ablation of NLRP3 or ASC did not significantly impact on brain levels of IL 1β at the terminal stage of disease. Our results exclude a significant role for NLRP3 and ASC in prion pathogenesis and invalidate their claimed potential as therapeutic target against prion diseases. |
| first_indexed | 2024-04-14T06:30:59Z |
| format | Article |
| id | doaj.art-aeac15d5345b4026a64fb1f5d6485693 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T06:30:59Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-aeac15d5345b4026a64fb1f5d64856932022-12-22T02:07:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011720810.1371/journal.pone.0117208Prion pathogenesis in the absence of NLRP3/ASC inflammasomes.Mario NuvoloneSilvia SorcePetra SchwarzAdriano AguzziThe accumulation of the scrapie prion protein PrPSc, a misfolded conformer of the cellular prion protein PrPC, is a crucial feature of prion diseases. In the central nervous system, this process is accompanied by conspicuous microglia activation. The NLRP3 inflammasome is a multi-molecular complex which can sense heterogeneous pathogen-associated molecular patterns and culminates in the activation of caspase 1 and release of IL 1β. The NLRP3 inflammasome was reported to be essential for IL 1β release after in vitro exposure to the amyloidogenic peptide PrP106-126 and to recombinant PrP fibrils. We therefore studied the role of the NLRP3 inflammasome in a mouse model of prion infection. Upon intracerebral inoculation with scrapie prions (strain RML), mice lacking NLRP3 (Nlrp3-/-) or the inflammasome adaptor protein ASC (Pycard-/-) succumbed to scrapie with attack rates and incubation times similar to wild-type mice, and developed the classic histologic and biochemical features of prion diseases. Genetic ablation of NLRP3 or ASC did not significantly impact on brain levels of IL 1β at the terminal stage of disease. Our results exclude a significant role for NLRP3 and ASC in prion pathogenesis and invalidate their claimed potential as therapeutic target against prion diseases.http://europepmc.org/articles/PMC4324825?pdf=render |
| spellingShingle | Mario Nuvolone Silvia Sorce Petra Schwarz Adriano Aguzzi Prion pathogenesis in the absence of NLRP3/ASC inflammasomes. PLoS ONE |
| title | Prion pathogenesis in the absence of NLRP3/ASC inflammasomes. |
| title_full | Prion pathogenesis in the absence of NLRP3/ASC inflammasomes. |
| title_fullStr | Prion pathogenesis in the absence of NLRP3/ASC inflammasomes. |
| title_full_unstemmed | Prion pathogenesis in the absence of NLRP3/ASC inflammasomes. |
| title_short | Prion pathogenesis in the absence of NLRP3/ASC inflammasomes. |
| title_sort | prion pathogenesis in the absence of nlrp3 asc inflammasomes |
| url | http://europepmc.org/articles/PMC4324825?pdf=render |
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