Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain
The structure of pullulan, the extracellular a-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear a-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-a-D-glucosidic bonds. Occasionally some of maltotriosyl residues are...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2001-06-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.shd.org.yu/HtDocs/SHD/Vol66/No6/V66-no6-03.pdf |
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| author | MIRJANA HRANISAVLJEVIC-JAKOVLJEVIC MILANKA RADULOVIC DRAGICA JAKOVLJEVIC MIROSLAV M. VRVIC |
| author_facet | MIRJANA HRANISAVLJEVIC-JAKOVLJEVIC MILANKA RADULOVIC DRAGICA JAKOVLJEVIC MIROSLAV M. VRVIC |
| author_sort | MIRJANA HRANISAVLJEVIC-JAKOVLJEVIC |
| collection | DOAJ |
| description | The structure of pullulan, the extracellular a-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear a-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-a-D-glucosidic bonds. Occasionally some of maltotriosyl residues are replaced by higher oligosaccharide units, most frequently with maltotetraosyl residues. Using the susceptibility of pullulan CH-1 (obtained from strain CH-1 of Aureobasidium pullulans) to hydrolysis catalysed by porcine alpha-amylase, the polysaccharide was cleaved and the fragments obtained fractionated by gel-permeation chromatography. The heterogenous size of the fragments indicates that there is no apparent regular distribution of tetrasaccharide units in the pullulan chain. Enzymatic digestion of pullulan CH-1 using pullulanase, followed by gel-permeation chromatography of the resulting digest confirmed these results as did preparative paper chromatography and CI mass spectrometry of the separated components, i.e., that maltotetraosyl units (about 7 %) are building units of pullulan CH-1. |
| first_indexed | 2024-12-11T12:35:06Z |
| format | Article |
| id | doaj.art-aeac7b7dd9e24eacb166dda54da38ecd |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-12-11T12:35:06Z |
| publishDate | 2001-06-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-aeac7b7dd9e24eacb166dda54da38ecd2022-12-22T01:07:09ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392001-06-01666377383Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strainMIRJANA HRANISAVLJEVIC-JAKOVLJEVICMILANKA RADULOVICDRAGICA JAKOVLJEVICMIROSLAV M. VRVICThe structure of pullulan, the extracellular a-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear a-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-a-D-glucosidic bonds. Occasionally some of maltotriosyl residues are replaced by higher oligosaccharide units, most frequently with maltotetraosyl residues. Using the susceptibility of pullulan CH-1 (obtained from strain CH-1 of Aureobasidium pullulans) to hydrolysis catalysed by porcine alpha-amylase, the polysaccharide was cleaved and the fragments obtained fractionated by gel-permeation chromatography. The heterogenous size of the fragments indicates that there is no apparent regular distribution of tetrasaccharide units in the pullulan chain. Enzymatic digestion of pullulan CH-1 using pullulanase, followed by gel-permeation chromatography of the resulting digest confirmed these results as did preparative paper chromatography and CI mass spectrometry of the separated components, i.e., that maltotetraosyl units (about 7 %) are building units of pullulan CH-1.http://www.shd.org.yu/HtDocs/SHD/Vol66/No6/V66-no6-03.pdfAureobasidium pullulanspolysaccharidepullulanamylolysispullulanolysis |
| spellingShingle | MIRJANA HRANISAVLJEVIC-JAKOVLJEVIC MILANKA RADULOVIC DRAGICA JAKOVLJEVIC MIROSLAV M. VRVIC Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain Journal of the Serbian Chemical Society Aureobasidium pullulans polysaccharide pullulan amylolysis pullulanolysis |
| title | Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain |
| title_full | Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain |
| title_fullStr | Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain |
| title_full_unstemmed | Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain |
| title_short | Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain |
| title_sort | fine structural analysis of the fungal polysaccharide pullulan elaborated by aureobasidium pullulans ch 1 strain |
| topic | Aureobasidium pullulans polysaccharide pullulan amylolysis pullulanolysis |
| url | http://www.shd.org.yu/HtDocs/SHD/Vol66/No6/V66-no6-03.pdf |
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