Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates
Background Mycobacterium avium subsp. hominissuis (MAH) is an emerging opportunistic human pathogen. It can cause pulmonary infections, lymphadenitis and disseminated infections in immuno-compromised patients. In addition, MAH is widespread in the environment, since it has been isolated from water,...
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| Format: | Article |
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PeerJ Inc.
2017-01-01
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| Online Access: | https://peerj.com/articles/2833.pdf |
| _version_ | 1797425005714210816 |
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| author | Andrea Sanchini Flavia Dematheis Torsten Semmler Astrid Lewin |
| author_facet | Andrea Sanchini Flavia Dematheis Torsten Semmler Astrid Lewin |
| author_sort | Andrea Sanchini |
| collection | DOAJ |
| description | Background Mycobacterium avium subsp. hominissuis (MAH) is an emerging opportunistic human pathogen. It can cause pulmonary infections, lymphadenitis and disseminated infections in immuno-compromised patients. In addition, MAH is widespread in the environment, since it has been isolated from water, soil or dust. In recent years, knowledge on MAH at the molecular level has increased substantially. In contrast, knowledge of the MAH metabolic phenotypes remains limited. Methods In this study, for the first time we analyzed the metabolic substrate utilization of ten MAH isolates, five from a clinical source and five from an environmental source. We used BIOLOG Phenotype MicroarrayTM technology for the analysis. This technology permits the rapid and global analysis of metabolic phenotypes. Results The ten MAH isolates tested showed different metabolic patterns pointing to high intra-species diversity. Our MAH isolates preferred to use fatty acids such as Tween, caproic, butyric and propionic acid as a carbon source, and L-cysteine as a nitrogen source. Environmental MAH isolates resulted in being more metabolically active than clinical isolates, since the former metabolized more strongly butyric acid (p = 0.0209) and propionic acid (p = 0.00307). Discussion Our study provides new insight into the metabolism of MAH. Understanding how bacteria utilize substrates during infection might help the developing of strategies to fight such infections. |
| first_indexed | 2024-03-09T08:09:45Z |
| format | Article |
| id | doaj.art-aeac8e8002d24eaea75518b1eaa84674 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:09:45Z |
| publishDate | 2017-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-aeac8e8002d24eaea75518b1eaa846742023-12-02T23:30:40ZengPeerJ Inc.PeerJ2167-83592017-01-015e283310.7717/peerj.2833Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolatesAndrea Sanchini0Flavia Dematheis1Torsten Semmler2Astrid Lewin3Division 16, Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute, Berlin, GermanyInstitute of Microbiology and Epizootics, Free University Berlin, Berlin, GermanyNG 1 Microbial Genomics, Robert Koch Institute, Berlin, GermanyDivision 16, Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute, Berlin, GermanyBackground Mycobacterium avium subsp. hominissuis (MAH) is an emerging opportunistic human pathogen. It can cause pulmonary infections, lymphadenitis and disseminated infections in immuno-compromised patients. In addition, MAH is widespread in the environment, since it has been isolated from water, soil or dust. In recent years, knowledge on MAH at the molecular level has increased substantially. In contrast, knowledge of the MAH metabolic phenotypes remains limited. Methods In this study, for the first time we analyzed the metabolic substrate utilization of ten MAH isolates, five from a clinical source and five from an environmental source. We used BIOLOG Phenotype MicroarrayTM technology for the analysis. This technology permits the rapid and global analysis of metabolic phenotypes. Results The ten MAH isolates tested showed different metabolic patterns pointing to high intra-species diversity. Our MAH isolates preferred to use fatty acids such as Tween, caproic, butyric and propionic acid as a carbon source, and L-cysteine as a nitrogen source. Environmental MAH isolates resulted in being more metabolically active than clinical isolates, since the former metabolized more strongly butyric acid (p = 0.0209) and propionic acid (p = 0.00307). Discussion Our study provides new insight into the metabolism of MAH. Understanding how bacteria utilize substrates during infection might help the developing of strategies to fight such infections.https://peerj.com/articles/2833.pdfMycobacterium aviumBiologOmniLog®MetabolismPhenotype microarray |
| spellingShingle | Andrea Sanchini Flavia Dematheis Torsten Semmler Astrid Lewin Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates PeerJ Mycobacterium avium Biolog OmniLog® Metabolism Phenotype microarray |
| title | Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates |
| title_full | Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates |
| title_fullStr | Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates |
| title_full_unstemmed | Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates |
| title_short | Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates |
| title_sort | metabolic phenotype of clinical and environmental mycobacterium avium subsp hominissuis isolates |
| topic | Mycobacterium avium Biolog OmniLog® Metabolism Phenotype microarray |
| url | https://peerj.com/articles/2833.pdf |
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