Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene Expression
Viral infections are described as modifying host gene expression; however, there is limited insight regarding rotavirus (RV) infections. This study aimed to assess the changes in intestinal gene expression after RV infection in a preclinical model, and the effect of 2-fucosyllactose (2′-FL) on this...
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MDPI AG
2023-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/8/1996 |
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| author | Laura Sáez-Fuertes Ignasi Azagra-Boronat Malén Massot-Cladera Karen Knipping Johan Garssen Àngels Franch Margarida Castell Francisco J. Pérez-Cano María J. Rodríguez-Lagunas |
| author_facet | Laura Sáez-Fuertes Ignasi Azagra-Boronat Malén Massot-Cladera Karen Knipping Johan Garssen Àngels Franch Margarida Castell Francisco J. Pérez-Cano María J. Rodríguez-Lagunas |
| author_sort | Laura Sáez-Fuertes |
| collection | DOAJ |
| description | Viral infections are described as modifying host gene expression; however, there is limited insight regarding rotavirus (RV) infections. This study aimed to assess the changes in intestinal gene expression after RV infection in a preclinical model, and the effect of 2-fucosyllactose (2′-FL) on this process. From days 2 to 8 of life, rats were supplemented with the dietary oligosaccharide 2′-FL or vehicle. In addition, an RV was inoculated on day 5 to nonsupplemented animals (RV group) and to 2′-FL-fed animals (RV+2′-FL group). Incidence and severity of diarrhea were established. A portion from the middle part of the small intestine was excised for gene expression analysis by microarray kit and qPCR. In nonsupplemented animals, RV-induced diarrhea upregulated host antiviral genes (e.g., <i>Oas1a</i>, <i>Irf7</i>, <i>Ifi44</i>, <i>Isg15</i>) and downregulated several genes involved in absorptive processes and intestinal maturation (e.g., <i>Onecut2</i>, and <i>Ccl19</i>). The 2′-FL-supplemented and infected animals had less diarrhea; however, their gene expression was affected in a similar way as the control-infected animals, with the exception of some immunity/maturation markers that were differentially expressed (e.g., <i>Ccl12</i> and <i>Afp</i>). Overall, assessing the expression of these key genes may be useful in the evaluation of the efficacy of nutritional interventions or treatments for RV infection. |
| first_indexed | 2024-03-11T04:39:35Z |
| format | Article |
| id | doaj.art-aeb467a816fb404dad0488e6987e9512 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T04:39:35Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-aeb467a816fb404dad0488e6987e95122023-11-17T20:47:27ZengMDPI AGNutrients2072-66432023-04-01158199610.3390/nu15081996Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene ExpressionLaura Sáez-Fuertes0Ignasi Azagra-Boronat1Malén Massot-Cladera2Karen Knipping3Johan Garssen4Àngels Franch5Margarida Castell6Francisco J. Pérez-Cano7María J. Rodríguez-Lagunas8Physiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, SpainPhysiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, SpainPhysiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, SpainDanone Nutricia Research, 3584 CT Utrecht, The NetherlandsDanone Nutricia Research, 3584 CT Utrecht, The NetherlandsPhysiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, SpainPhysiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, SpainPhysiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, SpainPhysiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, SpainViral infections are described as modifying host gene expression; however, there is limited insight regarding rotavirus (RV) infections. This study aimed to assess the changes in intestinal gene expression after RV infection in a preclinical model, and the effect of 2-fucosyllactose (2′-FL) on this process. From days 2 to 8 of life, rats were supplemented with the dietary oligosaccharide 2′-FL or vehicle. In addition, an RV was inoculated on day 5 to nonsupplemented animals (RV group) and to 2′-FL-fed animals (RV+2′-FL group). Incidence and severity of diarrhea were established. A portion from the middle part of the small intestine was excised for gene expression analysis by microarray kit and qPCR. In nonsupplemented animals, RV-induced diarrhea upregulated host antiviral genes (e.g., <i>Oas1a</i>, <i>Irf7</i>, <i>Ifi44</i>, <i>Isg15</i>) and downregulated several genes involved in absorptive processes and intestinal maturation (e.g., <i>Onecut2</i>, and <i>Ccl19</i>). The 2′-FL-supplemented and infected animals had less diarrhea; however, their gene expression was affected in a similar way as the control-infected animals, with the exception of some immunity/maturation markers that were differentially expressed (e.g., <i>Ccl12</i> and <i>Afp</i>). Overall, assessing the expression of these key genes may be useful in the evaluation of the efficacy of nutritional interventions or treatments for RV infection.https://www.mdpi.com/2072-6643/15/8/1996rotavirusinfection2-fucosyllactoseoligosaccharide |
| spellingShingle | Laura Sáez-Fuertes Ignasi Azagra-Boronat Malén Massot-Cladera Karen Knipping Johan Garssen Àngels Franch Margarida Castell Francisco J. Pérez-Cano María J. Rodríguez-Lagunas Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene Expression Nutrients rotavirus infection 2-fucosyllactose oligosaccharide |
| title | Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene Expression |
| title_full | Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene Expression |
| title_fullStr | Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene Expression |
| title_full_unstemmed | Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene Expression |
| title_short | Effect of Rotavirus Infection and 2′-Fucosyllactose Administration on Rat Intestinal Gene Expression |
| title_sort | effect of rotavirus infection and 2 fucosyllactose administration on rat intestinal gene expression |
| topic | rotavirus infection 2-fucosyllactose oligosaccharide |
| url | https://www.mdpi.com/2072-6643/15/8/1996 |
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