External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging system
Drug distribution is a vital parameter for depicting the pharmacokinetic properties of therapeutic agents. Magnetic nanoparticles (MNPs) conjugated with anti-cluster of differentiation 3 (anti-CD3) monoclonal antibody (MNPs-Ab) is a promising immunotherapeutic agent. Magnet-guided targeted delivery...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2024-01-01
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| Series: | AIP Advances |
| Online Access: | http://dx.doi.org/10.1063/9.0000638 |
| _version_ | 1827363174449414144 |
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| author | Mahbub Hasan Jong-Gu Choi Sang-Suk Lee |
| author_facet | Mahbub Hasan Jong-Gu Choi Sang-Suk Lee |
| author_sort | Mahbub Hasan |
| collection | DOAJ |
| description | Drug distribution is a vital parameter for depicting the pharmacokinetic properties of therapeutic agents. Magnetic nanoparticles (MNPs) conjugated with anti-cluster of differentiation 3 (anti-CD3) monoclonal antibody (MNPs-Ab) is a promising immunotherapeutic agent. Magnet-guided targeted delivery of MNPs has been studied exclusively in oncology. The distribution of MNPs is routinely analyzed by measuring Fe levels using inductively coupled mass spectrometry (ICP-MS). However, the ICP-based methods measure the total iron content, including endogenous iron. We used an efficient approach that measured MNPs-Ab distribution after intravenous administration using an in vivo imaging system. The fluorescent-labeled anti-CD3s are conjugated with dextran-coated magnetic nanoparticles (50 nm diameter) by the glutaraldehyde conjugation method. MNPs-Ab was intravenously administered to BALB/c mice. The fluorescence intensity in live animal and organs were analyzed after 0, 1, and 24 h. The conjugation of fluorescently labeled antibodies with MNPs was validated. The attachment of a permanent magnet on the dorsal skin area successfully guided the intravenously injected Ab-MNPs within 24 h. However, after 1 h, no observable fluorescence was seen at the target site. The in vitro organ distribution study confirms nanoparticles’ predominant distribution in the liver and kidneys-the intensities of the fluorescence increase after 24 h than 1 h. Our in vivo imaging studies confirmed the external magnet-driven targeted distribution of the antibody-conjugated nanoparticle. Besides, the in vivo imaging system enables rapidly identifying the distributed nanoparticles. |
| first_indexed | 2024-03-08T07:43:11Z |
| format | Article |
| id | doaj.art-aeb6dd9c6ca244fe8aac9e590231344e |
| institution | Directory Open Access Journal |
| issn | 2158-3226 |
| language | English |
| last_indexed | 2024-03-08T07:43:11Z |
| publishDate | 2024-01-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | AIP Advances |
| spelling | doaj.art-aeb6dd9c6ca244fe8aac9e590231344e2024-02-02T16:46:06ZengAIP Publishing LLCAIP Advances2158-32262024-01-01141015128015128-510.1063/9.0000638External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging systemMahbub Hasan0Jong-Gu Choi1Sang-Suk Lee2Department of Digital Healthcare Engineering, College of Health Sciences, Sangji University, Wonju 26339, Republic of KoreaDepartment of Digital Healthcare Engineering, College of Health Sciences, Sangji University, Wonju 26339, Republic of KoreaDepartment of Digital Healthcare Engineering, College of Health Sciences, Sangji University, Wonju 26339, Republic of KoreaDrug distribution is a vital parameter for depicting the pharmacokinetic properties of therapeutic agents. Magnetic nanoparticles (MNPs) conjugated with anti-cluster of differentiation 3 (anti-CD3) monoclonal antibody (MNPs-Ab) is a promising immunotherapeutic agent. Magnet-guided targeted delivery of MNPs has been studied exclusively in oncology. The distribution of MNPs is routinely analyzed by measuring Fe levels using inductively coupled mass spectrometry (ICP-MS). However, the ICP-based methods measure the total iron content, including endogenous iron. We used an efficient approach that measured MNPs-Ab distribution after intravenous administration using an in vivo imaging system. The fluorescent-labeled anti-CD3s are conjugated with dextran-coated magnetic nanoparticles (50 nm diameter) by the glutaraldehyde conjugation method. MNPs-Ab was intravenously administered to BALB/c mice. The fluorescence intensity in live animal and organs were analyzed after 0, 1, and 24 h. The conjugation of fluorescently labeled antibodies with MNPs was validated. The attachment of a permanent magnet on the dorsal skin area successfully guided the intravenously injected Ab-MNPs within 24 h. However, after 1 h, no observable fluorescence was seen at the target site. The in vitro organ distribution study confirms nanoparticles’ predominant distribution in the liver and kidneys-the intensities of the fluorescence increase after 24 h than 1 h. Our in vivo imaging studies confirmed the external magnet-driven targeted distribution of the antibody-conjugated nanoparticle. Besides, the in vivo imaging system enables rapidly identifying the distributed nanoparticles.http://dx.doi.org/10.1063/9.0000638 |
| spellingShingle | Mahbub Hasan Jong-Gu Choi Sang-Suk Lee External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging system AIP Advances |
| title | External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging system |
| title_full | External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging system |
| title_fullStr | External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging system |
| title_full_unstemmed | External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging system |
| title_short | External magnet-guided targeted delivery and tissue distribution analysis of anti-cluster of differentiation 3 conjugated magnetic nanoparticles of Fe3O4 using in vivo imaging system |
| title_sort | external magnet guided targeted delivery and tissue distribution analysis of anti cluster of differentiation 3 conjugated magnetic nanoparticles of fe3o4 using in vivo imaging system |
| url | http://dx.doi.org/10.1063/9.0000638 |
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