Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress
Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cel...
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MDPI AG
2019-12-01
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author | Flávia Póvoa da Costa Bruna Puty Lygia S. Nogueira Geovanni Pereira Mitre Sávio Monteiro dos Santos Bruno José Brito Teixeira Maria Sueli da Silva Kataoka Manoela Domingues Martins Carlos Augusto Galvão Barboza Marta Chagas Monteiro Hervé Rogez Edivaldo Herculano Corrêa de Oliveira Rafael Rodrigues Lima |
author_facet | Flávia Póvoa da Costa Bruna Puty Lygia S. Nogueira Geovanni Pereira Mitre Sávio Monteiro dos Santos Bruno José Brito Teixeira Maria Sueli da Silva Kataoka Manoela Domingues Martins Carlos Augusto Galvão Barboza Marta Chagas Monteiro Hervé Rogez Edivaldo Herculano Corrêa de Oliveira Rafael Rodrigues Lima |
author_sort | Flávia Póvoa da Costa |
collection | DOAJ |
description | Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H<sub>2</sub>O<sub>2</sub> exposure, and H<sub>2</sub>O<sub>2</sub> exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 μM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 μM even in the presence of H<sub>2</sub>O<sub>2</sub>. In a concentration of 0.1 μM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 μM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide. |
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spelling | doaj.art-aeba898332be43458d87c0848bba5a362023-08-02T04:10:32ZengMDPI AGAntioxidants2076-39212019-12-01911610.3390/antiox9010016antiox9010016Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative StressFlávia Póvoa da Costa0Bruna Puty1Lygia S. Nogueira2Geovanni Pereira Mitre3Sávio Monteiro dos Santos4Bruno José Brito Teixeira5Maria Sueli da Silva Kataoka6Manoela Domingues Martins7Carlos Augusto Galvão Barboza8Marta Chagas Monteiro9Hervé Rogez10Edivaldo Herculano Corrêa de Oliveira11Rafael Rodrigues Lima12Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilLaboratory of Cell Culture, Faculty of Dentistry, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilLaboratory of Oxidative Stress and Clinical Immunology, Faculty of Pharmacy, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilCenter for Valorization of Amazonian Bioactive Compounds (CVACBA) & Federal University of Pará UFPA, Belém-Pará 66075-110, BrazilLaboratory of Cell Culture, Faculty of Dentistry, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilDepartment of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 91509-900, RS, BrazilDepartment of Morphology, Federal University of Rio Grande do Norte, Natal 59078-970, BrazilLaboratory of Oxidative Stress and Clinical Immunology, Faculty of Pharmacy, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilCenter for Valorization of Amazonian Bioactive Compounds (CVACBA) & Federal University of Pará UFPA, Belém-Pará 66075-110, BrazilLaboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua-Pará 67030-000, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, BrazilPiceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H<sub>2</sub>O<sub>2</sub> exposure, and H<sub>2</sub>O<sub>2</sub> exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 μM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 μM even in the presence of H<sub>2</sub>O<sub>2</sub>. In a concentration of 0.1 μM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 μM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide.https://www.mdpi.com/2076-3921/9/1/16stilbenesviabilityhydrogen peroxidepiceatannolperiodontal ligament |
spellingShingle | Flávia Póvoa da Costa Bruna Puty Lygia S. Nogueira Geovanni Pereira Mitre Sávio Monteiro dos Santos Bruno José Brito Teixeira Maria Sueli da Silva Kataoka Manoela Domingues Martins Carlos Augusto Galvão Barboza Marta Chagas Monteiro Hervé Rogez Edivaldo Herculano Corrêa de Oliveira Rafael Rodrigues Lima Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress Antioxidants stilbenes viability hydrogen peroxide piceatannol periodontal ligament |
title | Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_full | Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_fullStr | Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_full_unstemmed | Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_short | Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_sort | piceatannol increases antioxidant defense and reduces cell death in human periodontal ligament fibroblast under oxidative stress |
topic | stilbenes viability hydrogen peroxide piceatannol periodontal ligament |
url | https://www.mdpi.com/2076-3921/9/1/16 |
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