Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
Neural patterning involves regionalised cell specification. Recent studies indicate that cell dynamics play instrumental roles in neural pattern refinement and progression, but the impact of cell behaviour and morphogenesis on neural specification is not understood. Here we combine 4D analysis of ce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/25543 |
| _version_ | 1811180688734420992 |
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| author | Esteban Hoijman L Fargas Patrick Blader Berta Alsina |
| author_facet | Esteban Hoijman L Fargas Patrick Blader Berta Alsina |
| author_sort | Esteban Hoijman |
| collection | DOAJ |
| description | Neural patterning involves regionalised cell specification. Recent studies indicate that cell dynamics play instrumental roles in neural pattern refinement and progression, but the impact of cell behaviour and morphogenesis on neural specification is not understood. Here we combine 4D analysis of cell behaviours with dynamic quantification of proneural expression to uncover the construction of the zebrafish otic neurogenic domain. We identify pioneer cells expressing neurog1 outside the otic epithelium that migrate and ingress into the epithelialising placode to become the first otic neuronal progenitors. Subsequently, neighbouring cells express neurog1 inside the placode, and apical symmetric divisions amplify the specified pool. Interestingly, pioneer cells delaminate shortly after ingression. Ablation experiments reveal that pioneer cells promote neurog1 expression in other otic cells. Finally, ingression relies on the epithelialisation timing controlled by FGF activity. We propose a novel view for otic neurogenesis integrating cell dynamics whereby ingression of pioneer cells instructs neuronal specification. |
| first_indexed | 2024-04-11T09:07:30Z |
| format | Article |
| id | doaj.art-aebc425be0ee480c914d9811f280453b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:07:30Z |
| publishDate | 2017-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-aebc425be0ee480c914d9811f280453b2022-12-22T04:32:36ZengeLife Sciences Publications LtdeLife2050-084X2017-05-01610.7554/eLife.25543Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specificationEsteban Hoijman0https://orcid.org/0000-0003-1927-1989L Fargas1Patrick Blader2Berta Alsina3https://orcid.org/0000-0002-6997-1249Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, SpainDepartment of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, SpainCentre de Biologie du Développement, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, FranceDepartment of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, SpainNeural patterning involves regionalised cell specification. Recent studies indicate that cell dynamics play instrumental roles in neural pattern refinement and progression, but the impact of cell behaviour and morphogenesis on neural specification is not understood. Here we combine 4D analysis of cell behaviours with dynamic quantification of proneural expression to uncover the construction of the zebrafish otic neurogenic domain. We identify pioneer cells expressing neurog1 outside the otic epithelium that migrate and ingress into the epithelialising placode to become the first otic neuronal progenitors. Subsequently, neighbouring cells express neurog1 inside the placode, and apical symmetric divisions amplify the specified pool. Interestingly, pioneer cells delaminate shortly after ingression. Ablation experiments reveal that pioneer cells promote neurog1 expression in other otic cells. Finally, ingression relies on the epithelialisation timing controlled by FGF activity. We propose a novel view for otic neurogenesis integrating cell dynamics whereby ingression of pioneer cells instructs neuronal specification.https://elifesciences.org/articles/25543neuronal specificationcell dynamicsneurog1morphogenesisFGF |
| spellingShingle | Esteban Hoijman L Fargas Patrick Blader Berta Alsina Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification eLife neuronal specification cell dynamics neurog1 morphogenesis FGF |
| title | Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification |
| title_full | Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification |
| title_fullStr | Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification |
| title_full_unstemmed | Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification |
| title_short | Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification |
| title_sort | pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification |
| topic | neuronal specification cell dynamics neurog1 morphogenesis FGF |
| url | https://elifesciences.org/articles/25543 |
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