Association of Malassezia furfur with Onychomycosis patients In Baghdad, Iraq
Background:Onychomycosis is a fungal nail infection. It infected about 10 % of the adults. The prevalence of onychomycosis is around 5% of fungal infections. Malassezia furfur is a basidiomycetous yeast and a part of normal human skin flora. By using biochemical, immunological and molecular methods...
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| Format: | Article |
| Language: | English |
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College of medicine/ University of Diyala
2018-06-01
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| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | http://djm.uodiyala.edu.iq/index.php/djm/article/view/39 |
| _version_ | 1797454717442326528 |
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| author | Abbas M Ammari Noor Their Talib Anaam Fuad Hussein Eman Sabbah Abdul-Rahman |
| author_facet | Abbas M Ammari Noor Their Talib Anaam Fuad Hussein Eman Sabbah Abdul-Rahman |
| author_sort | Abbas M Ammari |
| collection | DOAJ |
| description |
Background:Onychomycosis is a fungal nail infection. It infected about 10 % of the adults. The prevalence of onychomycosis is around 5% of fungal infections. Malassezia furfur is a basidiomycetous yeast and a part of normal human skin flora. By using biochemical, immunological and molecular methods on Malassezia yeast, this genus comprises 14 species. The higher detection rate of Malassezia furfur was observed by using molecular identification methods, the internal transcribed spacer (ITS) and large subunit (LSU) regions.
Objective: To detect of Malassezia furfur associated with Onychomycosis and evaluate the application of molecular method as rapid identification of Malassezia furfur.
Patients and Methods:Fifty samples have been collected from patients presented signs of onychomycosis in Al- Imammian Al-Kadhamain Teaching Hospital, Baghdad, Iraq for a period of six months (from 1st of October to the 30rd of April). Clinical diagnosis was done by consultant dermatologist. Twenty nine were males and twenty one were females, the mean age of studied group was (26.12 ± 9.89) years old, with age ranging from 10 to 60 years old. Fifty healthy volunteers as control includes nail clips, the range of ages was from 1 to 60 years old with a mean of (27.88 ± 12.03) years. Patients and control were investigated for M. furfur using phenotypic and molecular analysis.
Results:Males were more infected with onychomycosis than females, with a frequency percentage (58%). According to age, the most frequent among (31-40 years old) age groups. On the basis of Polymerase chain reaction (PCR) method it was showed a positive result in 6 (12%) out of 50 onychomycosis patients and a positive result in 1 (2%) out of 50 healthy volunteers.
Conclusion:From these findings, it was concluded that the males were most infected than females. PCR technique is sensitive, specific and less time consuming than cultural methods .
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| first_indexed | 2024-03-09T15:42:04Z |
| format | Article |
| id | doaj.art-aebebd2dd9d641dabd2cd61bbc68e193 |
| institution | Directory Open Access Journal |
| issn | 2219-9764 2617-8982 |
| language | English |
| last_indexed | 2024-03-09T15:42:04Z |
| publishDate | 2018-06-01 |
| publisher | College of medicine/ University of Diyala |
| record_format | Article |
| series | Diyala Journal of Medicine |
| spelling | doaj.art-aebebd2dd9d641dabd2cd61bbc68e1932023-11-24T23:24:59ZengCollege of medicine/ University of DiyalaDiyala Journal of Medicine2219-97642617-89822018-06-01141Association of Malassezia furfur with Onychomycosis patients In Baghdad, IraqAbbas M AmmariNoor Their TalibAnaam Fuad HusseinEman Sabbah Abdul-Rahman Background:Onychomycosis is a fungal nail infection. It infected about 10 % of the adults. The prevalence of onychomycosis is around 5% of fungal infections. Malassezia furfur is a basidiomycetous yeast and a part of normal human skin flora. By using biochemical, immunological and molecular methods on Malassezia yeast, this genus comprises 14 species. The higher detection rate of Malassezia furfur was observed by using molecular identification methods, the internal transcribed spacer (ITS) and large subunit (LSU) regions. Objective: To detect of Malassezia furfur associated with Onychomycosis and evaluate the application of molecular method as rapid identification of Malassezia furfur. Patients and Methods:Fifty samples have been collected from patients presented signs of onychomycosis in Al- Imammian Al-Kadhamain Teaching Hospital, Baghdad, Iraq for a period of six months (from 1st of October to the 30rd of April). Clinical diagnosis was done by consultant dermatologist. Twenty nine were males and twenty one were females, the mean age of studied group was (26.12 ± 9.89) years old, with age ranging from 10 to 60 years old. Fifty healthy volunteers as control includes nail clips, the range of ages was from 1 to 60 years old with a mean of (27.88 ± 12.03) years. Patients and control were investigated for M. furfur using phenotypic and molecular analysis. Results:Males were more infected with onychomycosis than females, with a frequency percentage (58%). According to age, the most frequent among (31-40 years old) age groups. On the basis of Polymerase chain reaction (PCR) method it was showed a positive result in 6 (12%) out of 50 onychomycosis patients and a positive result in 1 (2%) out of 50 healthy volunteers. Conclusion:From these findings, it was concluded that the males were most infected than females. PCR technique is sensitive, specific and less time consuming than cultural methods . http://djm.uodiyala.edu.iq/index.php/djm/article/view/39Malassezia furfur, Onychomycosis, Healthy Volunteers. |
| spellingShingle | Abbas M Ammari Noor Their Talib Anaam Fuad Hussein Eman Sabbah Abdul-Rahman Association of Malassezia furfur with Onychomycosis patients In Baghdad, Iraq Diyala Journal of Medicine Malassezia furfur, Onychomycosis, Healthy Volunteers. |
| title | Association of Malassezia furfur with Onychomycosis patients In Baghdad, Iraq |
| title_full | Association of Malassezia furfur with Onychomycosis patients In Baghdad, Iraq |
| title_fullStr | Association of Malassezia furfur with Onychomycosis patients In Baghdad, Iraq |
| title_full_unstemmed | Association of Malassezia furfur with Onychomycosis patients In Baghdad, Iraq |
| title_short | Association of Malassezia furfur with Onychomycosis patients In Baghdad, Iraq |
| title_sort | association of malassezia furfur with onychomycosis patients in baghdad iraq |
| topic | Malassezia furfur, Onychomycosis, Healthy Volunteers. |
| url | http://djm.uodiyala.edu.iq/index.php/djm/article/view/39 |
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