TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46
MYB/c-MYB is a proto-oncogene encoding a helix-turn-helix transcription factor that plays a critical role in controlling proliferation and multilineage differentiation of hematopoietic progenitor and stem cells. Deregulation of MYB expression is associated with several types of leukemias and lymphom...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-07-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120301550 |
| _version_ | 1818954316357042176 |
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| author | Chenyu Fan Zahir Shah Hanif Ullah Elena S. Philonenko Baoyun Zhang Ying Tan Cuihua Wang Jianguang Zhang Igor M. Samokhvalov |
| author_facet | Chenyu Fan Zahir Shah Hanif Ullah Elena S. Philonenko Baoyun Zhang Ying Tan Cuihua Wang Jianguang Zhang Igor M. Samokhvalov |
| author_sort | Chenyu Fan |
| collection | DOAJ |
| description | MYB/c-MYB is a proto-oncogene encoding a helix-turn-helix transcription factor that plays a critical role in controlling proliferation and multilineage differentiation of hematopoietic progenitor and stem cells. Deregulation of MYB expression is associated with several types of leukemias and lymphomas. In an attempt to explore the role of the gene in the early human hematopoiesis, we have achieved bi-allelic targeting of MYB in human embryonic stem cells (hESCs) by TALEN-mediated homologous recombination. Furthermore, the gene targeting introduced eYFP Venus reporter gene into the MYB locus to delineate the expression pattern of MYB. The resulting two cell lines, WAe001-A-45 and WAe001-A-46, passed the standard assays for human pluripotent stem cells. Hematopoietic differentiation of these cell lines provides a model to study the role of MYB in human hematopoietic development. |
| first_indexed | 2024-12-20T10:20:14Z |
| format | Article |
| id | doaj.art-aec3e8dcf1e9452eb2388c547f7351aa |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-20T10:20:14Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-aec3e8dcf1e9452eb2388c547f7351aa2022-12-21T19:43:57ZengElsevierStem Cell Research1873-50612020-07-0146101854TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46Chenyu Fan0Zahir Shah1Hanif Ullah2Elena S. Philonenko3Baoyun Zhang4Ying Tan5Cuihua Wang6Jianguang Zhang7Igor M. Samokhvalov8Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding author at: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.MYB/c-MYB is a proto-oncogene encoding a helix-turn-helix transcription factor that plays a critical role in controlling proliferation and multilineage differentiation of hematopoietic progenitor and stem cells. Deregulation of MYB expression is associated with several types of leukemias and lymphomas. In an attempt to explore the role of the gene in the early human hematopoiesis, we have achieved bi-allelic targeting of MYB in human embryonic stem cells (hESCs) by TALEN-mediated homologous recombination. Furthermore, the gene targeting introduced eYFP Venus reporter gene into the MYB locus to delineate the expression pattern of MYB. The resulting two cell lines, WAe001-A-45 and WAe001-A-46, passed the standard assays for human pluripotent stem cells. Hematopoietic differentiation of these cell lines provides a model to study the role of MYB in human hematopoietic development.http://www.sciencedirect.com/science/article/pii/S1873506120301550MYBGene targetingTALENhESCsin vitro differentiation |
| spellingShingle | Chenyu Fan Zahir Shah Hanif Ullah Elena S. Philonenko Baoyun Zhang Ying Tan Cuihua Wang Jianguang Zhang Igor M. Samokhvalov TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46 Stem Cell Research MYB Gene targeting TALEN hESCs in vitro differentiation |
| title | TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46 |
| title_full | TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46 |
| title_fullStr | TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46 |
| title_full_unstemmed | TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46 |
| title_short | TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46 |
| title_sort | talen mediated biallelic inactivation of myb in human embryonic stem cell lines wae001 a 45 and wae001 a 46 |
| topic | MYB Gene targeting TALEN hESCs in vitro differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S1873506120301550 |
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