Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples
Abstract This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temper...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-07-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100623&tlng=en |
| _version_ | 1811225573419122688 |
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| author | Flávia Viana Avelar Dutra Leila Suleimara Teixeira Bruna Carneiro Pires Diego Hernando Ângulo Florez Roseane Andrade Teixeira Keyller Bastos Borges |
| author_facet | Flávia Viana Avelar Dutra Leila Suleimara Teixeira Bruna Carneiro Pires Diego Hernando Ângulo Florez Roseane Andrade Teixeira Keyller Bastos Borges |
| author_sort | Flávia Viana Avelar Dutra |
| collection | DOAJ |
| description | Abstract This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temperature, flow rate, injection volume and mobile phase with different proportions of solvents were evaluated. The best chromatographic conditions were obtained using the Phenomenex® C18 (150 × 4.60 mm, 5 μm) column at a temperature of 20 °C, flow rate of 1.2 mL min-1, injection volume of 20 μL, and detection at 250 nm. Acetonitrile: ultrapure water (87: 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and acetonitrile: methanol: ultrapure water (53: 35: 12, v/v/v) for abamectin and ivermectin. Under these conditions satisfactory results were obtained, with appropriate limits of detection and quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy the need for analytical methods for the multi-determination of MBMs and the B1a and B1b forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and should aid in the development of the field. |
| first_indexed | 2024-04-12T09:09:36Z |
| format | Article |
| id | doaj.art-aec463043007448dbf2fecc394938a80 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-12T09:09:36Z |
| publishDate | 2022-07-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-aec463043007448dbf2fecc394938a802022-12-22T03:39:00ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-07-015810.1590/s2175-97902022e19587Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samplesFlávia Viana Avelar DutraLeila Suleimara TeixeiraBruna Carneiro PiresDiego Hernando Ângulo FlorezRoseane Andrade TeixeiraKeyller Bastos Borgeshttps://orcid.org/0000-0003-1067-1919Abstract This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temperature, flow rate, injection volume and mobile phase with different proportions of solvents were evaluated. The best chromatographic conditions were obtained using the Phenomenex® C18 (150 × 4.60 mm, 5 μm) column at a temperature of 20 °C, flow rate of 1.2 mL min-1, injection volume of 20 μL, and detection at 250 nm. Acetonitrile: ultrapure water (87: 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and acetonitrile: methanol: ultrapure water (53: 35: 12, v/v/v) for abamectin and ivermectin. Under these conditions satisfactory results were obtained, with appropriate limits of detection and quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy the need for analytical methods for the multi-determination of MBMs and the B1a and B1b forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and should aid in the development of the field.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100623&tlng=enAvermectinsMilbemycinsQuality controlBulk samples |
| spellingShingle | Flávia Viana Avelar Dutra Leila Suleimara Teixeira Bruna Carneiro Pires Diego Hernando Ângulo Florez Roseane Andrade Teixeira Keyller Bastos Borges Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples Brazilian Journal of Pharmaceutical Sciences Avermectins Milbemycins Quality control Bulk samples |
| title | Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples |
| title_full | Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples |
| title_fullStr | Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples |
| title_full_unstemmed | Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples |
| title_short | Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples |
| title_sort | development and validation of analytical methods by hplc for quality control of avermectins and milbemycins in bulk samples |
| topic | Avermectins Milbemycins Quality control Bulk samples |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100623&tlng=en |
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