Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model
Sophoraflavanone G (SG), isolated from <i>Sophora flavescens</i>, has anti-inflammatory and anti-tumor bioactive properties. We previously showed that SG promotes apoptosis in human breast cancer cells and leukemia cells and reduces the inflammatory response in lipopolysaccharide-stimula...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/1422-0067/23/11/6104 |
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| author | Meng-Chun Wang Wen-Chung Huang Li-Chen Chen Kuo-Wei Yeh Chwan-Fwu Lin Chian-Jiun Liou |
| author_facet | Meng-Chun Wang Wen-Chung Huang Li-Chen Chen Kuo-Wei Yeh Chwan-Fwu Lin Chian-Jiun Liou |
| author_sort | Meng-Chun Wang |
| collection | DOAJ |
| description | Sophoraflavanone G (SG), isolated from <i>Sophora flavescens</i>, has anti-inflammatory and anti-tumor bioactive properties. We previously showed that SG promotes apoptosis in human breast cancer cells and leukemia cells and reduces the inflammatory response in lipopolysaccharide-stimulated macrophages. We investigated whether SG attenuates airway hyper-responsiveness (AHR) and airway inflammation in asthmatic mice. We also assessed its effects on the anti-inflammatory response in human tracheal epithelial cells. Female BALB/c mice were sensitized with ovalbumin, and asthmatic mice were treated with SG by intraperitoneal injection. We also exposed human bronchial epithelial BEAS-2B cells to different concentrations of SG to evaluate its effects on inflammatory cytokine levels. SG treatment significantly reduced AHR, eosinophil infiltration, goblet cell hyperplasia, and airway inflammation in the lungs of asthmatic mice. In the lungs of ovalbumin-sensitized mice, SG significantly promoted superoxide dismutase and glutathione expression and attenuated malondialdehyde levels. SG also suppressed levels of Th2 cytokines and chemokines in lung and bronchoalveolar lavage samples. In addition, we confirmed that SG decreased pro-inflammatory cytokine, chemokine, and eotaxin expression in inflammatory BEAS-2B cells. Taken together, our data demonstrate that SG shows potential as an immunomodulator that can improve asthma symptoms by decreasing airway-inflammation-related oxidative stress. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:15:28Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-aec58e686f4d49f1973619b9f9e43ceb2023-11-23T14:09:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311610410.3390/ijms23116104Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma ModelMeng-Chun Wang0Wen-Chung Huang1Li-Chen Chen2Kuo-Wei Yeh3Chwan-Fwu Lin4Chian-Jiun Liou5Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Taoyuan 33378, TaiwanGraduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety, Chang Gung University of Science and Technology, Taoyuan 33303, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, TaiwanDepartment of Cosmetic Science, Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Taoyuan 33303, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, TaiwanSophoraflavanone G (SG), isolated from <i>Sophora flavescens</i>, has anti-inflammatory and anti-tumor bioactive properties. We previously showed that SG promotes apoptosis in human breast cancer cells and leukemia cells and reduces the inflammatory response in lipopolysaccharide-stimulated macrophages. We investigated whether SG attenuates airway hyper-responsiveness (AHR) and airway inflammation in asthmatic mice. We also assessed its effects on the anti-inflammatory response in human tracheal epithelial cells. Female BALB/c mice were sensitized with ovalbumin, and asthmatic mice were treated with SG by intraperitoneal injection. We also exposed human bronchial epithelial BEAS-2B cells to different concentrations of SG to evaluate its effects on inflammatory cytokine levels. SG treatment significantly reduced AHR, eosinophil infiltration, goblet cell hyperplasia, and airway inflammation in the lungs of asthmatic mice. In the lungs of ovalbumin-sensitized mice, SG significantly promoted superoxide dismutase and glutathione expression and attenuated malondialdehyde levels. SG also suppressed levels of Th2 cytokines and chemokines in lung and bronchoalveolar lavage samples. In addition, we confirmed that SG decreased pro-inflammatory cytokine, chemokine, and eotaxin expression in inflammatory BEAS-2B cells. Taken together, our data demonstrate that SG shows potential as an immunomodulator that can improve asthma symptoms by decreasing airway-inflammation-related oxidative stress.https://www.mdpi.com/1422-0067/23/11/6104airway hyper-responsivenessairway inflammationasthmasophoraflavanone GTh2 cell |
| spellingShingle | Meng-Chun Wang Wen-Chung Huang Li-Chen Chen Kuo-Wei Yeh Chwan-Fwu Lin Chian-Jiun Liou Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model International Journal of Molecular Sciences airway hyper-responsiveness airway inflammation asthma sophoraflavanone G Th2 cell |
| title | Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model |
| title_full | Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model |
| title_fullStr | Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model |
| title_full_unstemmed | Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model |
| title_short | Sophoraflavanone G from <i>Sophora flavescens</i> Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model |
| title_sort | sophoraflavanone g from i sophora flavescens i ameliorates allergic airway inflammation by suppressing th2 response and oxidative stress in a murine asthma model |
| topic | airway hyper-responsiveness airway inflammation asthma sophoraflavanone G Th2 cell |
| url | https://www.mdpi.com/1422-0067/23/11/6104 |
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