The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the Field
Vaccination is an effective approach to prevent, control and eradicate diseases, including lumpy skin disease (LSD). One of the measures to address farmer hesitation to vaccinate is guaranteeing the quality of vaccine batches. The purpose of this study was to demonstrate the importance of a quality...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/9/1019 |
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| author | Andy Haegeman Ilse De Leeuw Meruyert Saduakassova Willem Van Campe Laetitia Aerts Wannes Philips Akhmetzhan Sultanov Laurent Mostin Kris De Clercq |
| author_facet | Andy Haegeman Ilse De Leeuw Meruyert Saduakassova Willem Van Campe Laetitia Aerts Wannes Philips Akhmetzhan Sultanov Laurent Mostin Kris De Clercq |
| author_sort | Andy Haegeman |
| collection | DOAJ |
| description | Vaccination is an effective approach to prevent, control and eradicate diseases, including lumpy skin disease (LSD). One of the measures to address farmer hesitation to vaccinate is guaranteeing the quality of vaccine batches. The purpose of this study was to demonstrate the importance of a quality procedure via the evaluation of the LSD vaccine, Lumpivax (Kevevapi). The initial PCR screening revealed the presence of wild type LSD virus (LSDV) and goatpox virus (GTPV), in addition to vaccine LSDV. New phylogenetic PCRs were developed to characterize in detail the genomic content and a vaccination/challenge trial was conducted to evaluate the impact on efficacy and diagnostics. The characterization confirmed the presence of LSDV wild-, vaccine- and GTPV-like sequences in the vaccine vial and also in samples taken from the vaccinated animals. The analysis was also suggestive for the presence of GTPV-LSDV (vaccine/wild) recombinants. In addition, the LSDV status of some of the animal samples was greatly influenced by the differentiating real-PCR used and could result in misinterpretation. Although the vaccine was clinically protective, the viral genomic content of the vaccine (being it multiple Capripox viruses and/or recombinants) and the impact on the diagnostics casts serious doubts of its use in the field. |
| first_indexed | 2024-03-10T07:08:55Z |
| format | Article |
| id | doaj.art-aec62b63d56c43e3b219acc332921ef3 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T07:08:55Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-aec62b63d56c43e3b219acc332921ef32023-11-22T15:34:56ZengMDPI AGVaccines2076-393X2021-09-0199101910.3390/vaccines9091019The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the FieldAndy Haegeman0Ilse De Leeuw1Meruyert Saduakassova2Willem Van Campe3Laetitia Aerts4Wannes Philips5Akhmetzhan Sultanov6Laurent Mostin7Kris De Clercq8Unit of Exotic and Particular Diseases, Scientific Directorate Infectious Diseases in Animals, Sciensano, Groeselenberg 99, B-1180 Brussels, BelgiumUnit of Exotic and Particular Diseases, Scientific Directorate Infectious Diseases in Animals, Sciensano, Groeselenberg 99, B-1180 Brussels, BelgiumKazakh Scientific Research Veterinary Institute (KazSRVI//KazNIVI), Raiymbek ave. 223, Almaty 050016, KazakhstanExperimental Center Machelen, Scientific Directorate Infectious Diseases in Animals, Sciensano, Kerklaan 68, B-1830 Machelen, BelgiumEURL for Diseases Caused by Capripox Viruses, Scientific Directorate Infectious Diseases in Animals, Sciensano, Groeselenberg 99, B-1180 Brussels, BelgiumEURL for Diseases Caused by Capripox Viruses, Scientific Directorate Infectious Diseases in Animals, Sciensano, Groeselenberg 99, B-1180 Brussels, BelgiumKazakh Scientific Research Veterinary Institute (KazSRVI//KazNIVI), Raiymbek ave. 223, Almaty 050016, KazakhstanExperimental Center Machelen, Scientific Directorate Infectious Diseases in Animals, Sciensano, Kerklaan 68, B-1830 Machelen, BelgiumUnit of Exotic and Particular Diseases, Scientific Directorate Infectious Diseases in Animals, Sciensano, Groeselenberg 99, B-1180 Brussels, BelgiumVaccination is an effective approach to prevent, control and eradicate diseases, including lumpy skin disease (LSD). One of the measures to address farmer hesitation to vaccinate is guaranteeing the quality of vaccine batches. The purpose of this study was to demonstrate the importance of a quality procedure via the evaluation of the LSD vaccine, Lumpivax (Kevevapi). The initial PCR screening revealed the presence of wild type LSD virus (LSDV) and goatpox virus (GTPV), in addition to vaccine LSDV. New phylogenetic PCRs were developed to characterize in detail the genomic content and a vaccination/challenge trial was conducted to evaluate the impact on efficacy and diagnostics. The characterization confirmed the presence of LSDV wild-, vaccine- and GTPV-like sequences in the vaccine vial and also in samples taken from the vaccinated animals. The analysis was also suggestive for the presence of GTPV-LSDV (vaccine/wild) recombinants. In addition, the LSDV status of some of the animal samples was greatly influenced by the differentiating real-PCR used and could result in misinterpretation. Although the vaccine was clinically protective, the viral genomic content of the vaccine (being it multiple Capripox viruses and/or recombinants) and the impact on the diagnostics casts serious doubts of its use in the field.https://www.mdpi.com/2076-393X/9/9/1019vaccinequality controllumpy skin diseaserecombinant |
| spellingShingle | Andy Haegeman Ilse De Leeuw Meruyert Saduakassova Willem Van Campe Laetitia Aerts Wannes Philips Akhmetzhan Sultanov Laurent Mostin Kris De Clercq The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the Field Vaccines vaccine quality control lumpy skin disease recombinant |
| title | The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the Field |
| title_full | The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the Field |
| title_fullStr | The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the Field |
| title_full_unstemmed | The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the Field |
| title_short | The Importance of Quality Control of LSDV Live Attenuated Vaccines for Its Safe Application in the Field |
| title_sort | importance of quality control of lsdv live attenuated vaccines for its safe application in the field |
| topic | vaccine quality control lumpy skin disease recombinant |
| url | https://www.mdpi.com/2076-393X/9/9/1019 |
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