A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment
Hepatitis E virus is a prominent cause of viral hepatitis worldwide. In Western countries, most infections are asymptomatic. However, acute self-limiting hepatitis and chronic cases in immunocompromised individuals can occur. Studying HEV is challenging due to its difficulty to grow in cell culture....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/12/676 |
| _version_ | 1797379169807499264 |
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| author | Tatjana Locus Ellen Lambrecht Sophie Lamoral Sjarlotte Willems Steven Van Gucht Thomas Vanwolleghem Michael Peeters |
| author_facet | Tatjana Locus Ellen Lambrecht Sophie Lamoral Sjarlotte Willems Steven Van Gucht Thomas Vanwolleghem Michael Peeters |
| author_sort | Tatjana Locus |
| collection | DOAJ |
| description | Hepatitis E virus is a prominent cause of viral hepatitis worldwide. In Western countries, most infections are asymptomatic. However, acute self-limiting hepatitis and chronic cases in immunocompromised individuals can occur. Studying HEV is challenging due to its difficulty to grow in cell culture. Consequently, the detection of the virus mainly relies on RT-qPCR, which cannot differentiate between infectious and non-infectious particles. To overcome this problem, methods assessing viral integrity offer a possible solution to differentiate between intact and damaged viruses. This study aims at optimizing existing HEV cell culture models and RT-qPCR-based assays for selectively detecting intact virions to establish a reliable model for assessing HEV infectivity. In conclusion, these newly developed methods hold promise for enhancing food safety by identifying approaches for inactivating HEV in food processing, thereby increasing food safety measures. |
| first_indexed | 2024-03-08T20:18:21Z |
| format | Article |
| id | doaj.art-aec7b6a236464b5da1506d7c4fb49edd |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-08T20:18:21Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-aec7b6a236464b5da1506d7c4fb49edd2023-12-22T14:48:15ZengMDPI AGVeterinary Sciences2306-73812023-11-01101267610.3390/vetsci10120676A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity AssessmentTatjana Locus0Ellen Lambrecht1Sophie Lamoral2Sjarlotte Willems3Steven Van Gucht4Thomas Vanwolleghem5Michael Peeters6Fisheries and Food, Technology and Food Unit, Flemish Research Institute for Agriculture (ILVO), Brusselsesteenweg 370, 9090 Melle, BelgiumFisheries and Food, Technology and Food Unit, Flemish Research Institute for Agriculture (ILVO), Brusselsesteenweg 370, 9090 Melle, BelgiumSciensano, Infectious Diseases in Humans, Viral Diseases, Engelandstraat 642, 1180 Ukkel, BelgiumFisheries and Food, Technology and Food Unit, Flemish Research Institute for Agriculture (ILVO), Brusselsesteenweg 370, 9090 Melle, BelgiumSciensano, Infectious Diseases in Humans, Viral Diseases, Engelandstraat 642, 1180 Ukkel, BelgiumLaboratory of Experimental Medicine and Pediatrics, Viral Hepatitis Research Group, University of Antwerp, Drie Eikenstraat 655, 2650 Edegem, BelgiumSciensano, Infectious Diseases in Humans, Viral Diseases, Engelandstraat 642, 1180 Ukkel, BelgiumHepatitis E virus is a prominent cause of viral hepatitis worldwide. In Western countries, most infections are asymptomatic. However, acute self-limiting hepatitis and chronic cases in immunocompromised individuals can occur. Studying HEV is challenging due to its difficulty to grow in cell culture. Consequently, the detection of the virus mainly relies on RT-qPCR, which cannot differentiate between infectious and non-infectious particles. To overcome this problem, methods assessing viral integrity offer a possible solution to differentiate between intact and damaged viruses. This study aims at optimizing existing HEV cell culture models and RT-qPCR-based assays for selectively detecting intact virions to establish a reliable model for assessing HEV infectivity. In conclusion, these newly developed methods hold promise for enhancing food safety by identifying approaches for inactivating HEV in food processing, thereby increasing food safety measures.https://www.mdpi.com/2306-7381/10/12/676Hepatitis E viruscell culturecapsid integritygenome integrityPtCl<sub>4</sub>RNase A |
| spellingShingle | Tatjana Locus Ellen Lambrecht Sophie Lamoral Sjarlotte Willems Steven Van Gucht Thomas Vanwolleghem Michael Peeters A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment Veterinary Sciences Hepatitis E virus cell culture capsid integrity genome integrity PtCl<sub>4</sub> RNase A |
| title | A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment |
| title_full | A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment |
| title_fullStr | A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment |
| title_full_unstemmed | A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment |
| title_short | A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment |
| title_sort | multifaceted approach for evaluating hepatitis e virus infectivity in vitro cell culture and innovative molecular methods for integrity assessment |
| topic | Hepatitis E virus cell culture capsid integrity genome integrity PtCl<sub>4</sub> RNase A |
| url | https://www.mdpi.com/2306-7381/10/12/676 |
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