THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9
The aim of this study was to determine the role of the human neonatal receptor for the Fc fragment of IgG (hFcRn) as a common uncoating cellular receptor for echoviruses and coxsackievirus A9 during infection of human rhabdomyosarcoma (RD) cells. Material and methods. The protective effect of the hu...
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| Format: | Article |
| Language: | English |
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Central Research Institute for Epidemiology
2019-06-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/244/147 |
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| author | P. S. Usoltseva A. V. Alimov A. V. Rezaykin A. G. Sergeev A. V. Novoselov |
| author_facet | P. S. Usoltseva A. V. Alimov A. V. Rezaykin A. G. Sergeev A. V. Novoselov |
| author_sort | P. S. Usoltseva |
| collection | DOAJ |
| description | The aim of this study was to determine the role of the human neonatal receptor for the Fc fragment of IgG (hFcRn) as a common uncoating cellular receptor for echoviruses and coxsackievirus A9 during infection of human rhabdomyosarcoma (RD) cells. Material and methods. The protective effect of the human serum albumin, purified from globulins, (HSA-GF) and antibodies to hFcRn was studied in RD cells infected with several strains and clones of species B enteroviruses possessing different receptor specificity (echoviruses 3, 9, 11, 30 and coxsackieviruses A9, B4, B5). Results. It was shown that HSA-GF at concentrations of 4% or less protected RD cells from infection with echoviruses 3, 9, 11 and coxsackievirus A9. The antibodies to hFcRn at concentrations of 2.5 ug/mL or less demonstrated the similar spectrum of protective activity in RD cells against infection with echoviruses 3, 9, 11, 30 and coxsackievirus A9. The protective effect of HSA-GF or the antibodies to hFcRn was not observed in RD cells infected with coxsackieviruses B4 and B5 that need coxsackievirus-adenovirus receptor for uncoating. Discussion. The usage of the previously characterized echovirus 11 clonal variants with different receptor specificity allowed us to define the function of hFcRn as a canyon-binding uncoating receptor in RD cells. The kinetics and magnitude of the observed protective effects correlated with receptor specificity of the enteroviruses used in this work supporting the two-step interaction of DAF-dependent echoviruses with the cellular receptors. Conclusions. In this study, the function of hFcRn was defined in RD cells as a canyon-binding and uncoating receptor for echoviruses and coxsackievirus A9. The two-step interaction of DAF-dependent echoviruses during entry into the cells was confirmed: initially with the binding receptor DAF and subsequently with the uncoating receptor hFcRn. |
| first_indexed | 2024-03-13T00:08:58Z |
| format | Article |
| id | doaj.art-aec7deead2ed469880ec024635774ecf |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:58Z |
| publishDate | 2019-06-01 |
| publisher | Central Research Institute for Epidemiology |
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| series | Вопросы вирусологии |
| spelling | doaj.art-aec7deead2ed469880ec024635774ecf2023-07-12T19:59:04ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972019-06-0164313213910.18821/0507-4088-2019-64-3-132-139224THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9P. S. Usoltseva0A. V. Alimov1A. V. Rezaykin2A. G. Sergeev3A. V. Novoselov4Yekaterinburg Scientific Research Institute of Viral InfectionsYekaterinburg Scientific Research Institute of Viral InfectionsYekaterinburg Scientific Research Institute of Viral InfectionsYekaterinburg Scientific Research Institute of Viral InfectionsYekaterinburg Scientific Research Institute of Viral InfectionsThe aim of this study was to determine the role of the human neonatal receptor for the Fc fragment of IgG (hFcRn) as a common uncoating cellular receptor for echoviruses and coxsackievirus A9 during infection of human rhabdomyosarcoma (RD) cells. Material and methods. The protective effect of the human serum albumin, purified from globulins, (HSA-GF) and antibodies to hFcRn was studied in RD cells infected with several strains and clones of species B enteroviruses possessing different receptor specificity (echoviruses 3, 9, 11, 30 and coxsackieviruses A9, B4, B5). Results. It was shown that HSA-GF at concentrations of 4% or less protected RD cells from infection with echoviruses 3, 9, 11 and coxsackievirus A9. The antibodies to hFcRn at concentrations of 2.5 ug/mL or less demonstrated the similar spectrum of protective activity in RD cells against infection with echoviruses 3, 9, 11, 30 and coxsackievirus A9. The protective effect of HSA-GF or the antibodies to hFcRn was not observed in RD cells infected with coxsackieviruses B4 and B5 that need coxsackievirus-adenovirus receptor for uncoating. Discussion. The usage of the previously characterized echovirus 11 clonal variants with different receptor specificity allowed us to define the function of hFcRn as a canyon-binding uncoating receptor in RD cells. The kinetics and magnitude of the observed protective effects correlated with receptor specificity of the enteroviruses used in this work supporting the two-step interaction of DAF-dependent echoviruses with the cellular receptors. Conclusions. In this study, the function of hFcRn was defined in RD cells as a canyon-binding and uncoating receptor for echoviruses and coxsackievirus A9. The two-step interaction of DAF-dependent echoviruses during entry into the cells was confirmed: initially with the binding receptor DAF and subsequently with the uncoating receptor hFcRn.https://virusjour.crie.ru/jour/article/viewFile/244/147fcrndafechoviruscoxsackievirusuncoating cellular receptorfcrndafalbumin |
| spellingShingle | P. S. Usoltseva A. V. Alimov A. V. Rezaykin A. G. Sergeev A. V. Novoselov THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9 Вопросы вирусологии fcrn daf echovirus coxsackievirus uncoating cellular receptor fcrn daf albumin |
| title | THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9 |
| title_full | THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9 |
| title_fullStr | THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9 |
| title_full_unstemmed | THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9 |
| title_short | THE ROLE OF THE NEONATAL FC RECEPTOR IN THE UNCOATING OF ECHOVIRUSES AND COXSACKIEVIRUS A9 |
| title_sort | role of the neonatal fc receptor in the uncoating of echoviruses and coxsackievirus a9 |
| topic | fcrn daf echovirus coxsackievirus uncoating cellular receptor fcrn daf albumin |
| url | https://virusjour.crie.ru/jour/article/viewFile/244/147 |
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