PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root Elongation
Auxin, the first discovered phytohormone, is important for the growth and development of plants through the establishment of homeostasis and asymmetry. Here, we cloned the auxin transporter gene <i>PIN-FORMED3</i> (<i>PIN3</i>) from the valuable timber tree hybrid <i>Li...
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MDPI AG
2022-04-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/13/4/568 |
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| author | Rui Li Yan Pan Lingfeng Hu Dingjie Yang Mengjian Yuan Zhaodong Hao Ye Lu Fuming Xiao Jisen Shi Jinhui Chen |
| author_facet | Rui Li Yan Pan Lingfeng Hu Dingjie Yang Mengjian Yuan Zhaodong Hao Ye Lu Fuming Xiao Jisen Shi Jinhui Chen |
| author_sort | Rui Li |
| collection | DOAJ |
| description | Auxin, the first discovered phytohormone, is important for the growth and development of plants through the establishment of homeostasis and asymmetry. Here, we cloned the auxin transporter gene <i>PIN-FORMED3</i> (<i>PIN3</i>) from the valuable timber tree hybrid <i>Liriodendron</i> (<i>Liriodendron chinense</i> × <i>Liriodendron tulipifera</i>). The gene contained a complete open reading frame of 1917 bp that encoded 638 amino acids. Phylogenetic analysis indicated that LhPIN3 exhibited the highest sequence similarity to the PIN3 of <i>Vitis vinifera</i>. Quantitative real-time PCR analysis showed that <i>LhPIN3</i> was broadly expressed across different tissues/organs of <i>Liriodendron</i>, with the highest expression level in the roots. Heterologous overexpression of <i>LhPIN3</i> in <i>Arabidopsis thaliana</i> caused considerable phenotypic changes, such as the root length and number of flowers. Genetic complementation of <i>Arabidopsis</i> <i>pin1</i> mutants by <i>LhPIN3</i>, driven by the cauliflower mosaic virus 35S promoter, fully restored the root length and number of flowers of the <i>pin1</i> mutant. Overall, our findings reveal that <i>LhPIN3</i> has similar capacities to regulate the root length and number of flowers of <i>Arabidopsis</i> with <i>AtPIN1</i>. |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-09T10:36:30Z |
| publishDate | 2022-04-01 |
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| series | Forests |
| spelling | doaj.art-aecd4607725244128582e7333099dbfb2023-12-01T20:54:43ZengMDPI AGForests1999-49072022-04-0113456810.3390/f13040568PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root ElongationRui Li0Yan Pan1Lingfeng Hu2Dingjie Yang3Mengjian Yuan4Zhaodong Hao5Ye Lu6Fuming Xiao7Jisen Shi8Jinhui Chen9Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaJiangxi Provincial Key Laboratory of Plant Biotechnology, Jiangxi Academy of Forestry, Nanchang 330032, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaAuxin, the first discovered phytohormone, is important for the growth and development of plants through the establishment of homeostasis and asymmetry. Here, we cloned the auxin transporter gene <i>PIN-FORMED3</i> (<i>PIN3</i>) from the valuable timber tree hybrid <i>Liriodendron</i> (<i>Liriodendron chinense</i> × <i>Liriodendron tulipifera</i>). The gene contained a complete open reading frame of 1917 bp that encoded 638 amino acids. Phylogenetic analysis indicated that LhPIN3 exhibited the highest sequence similarity to the PIN3 of <i>Vitis vinifera</i>. Quantitative real-time PCR analysis showed that <i>LhPIN3</i> was broadly expressed across different tissues/organs of <i>Liriodendron</i>, with the highest expression level in the roots. Heterologous overexpression of <i>LhPIN3</i> in <i>Arabidopsis thaliana</i> caused considerable phenotypic changes, such as the root length and number of flowers. Genetic complementation of <i>Arabidopsis</i> <i>pin1</i> mutants by <i>LhPIN3</i>, driven by the cauliflower mosaic virus 35S promoter, fully restored the root length and number of flowers of the <i>pin1</i> mutant. Overall, our findings reveal that <i>LhPIN3</i> has similar capacities to regulate the root length and number of flowers of <i>Arabidopsis</i> with <i>AtPIN1</i>.https://www.mdpi.com/1999-4907/13/4/568<i>Liriodendron chinense</i> × <i>Liriodendron tulipifer</i><i>Arabidopsis thaliana</i>PIN3rootflower |
| spellingShingle | Rui Li Yan Pan Lingfeng Hu Dingjie Yang Mengjian Yuan Zhaodong Hao Ye Lu Fuming Xiao Jisen Shi Jinhui Chen PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root Elongation Forests <i>Liriodendron chinense</i> × <i>Liriodendron tulipifer</i> <i>Arabidopsis thaliana</i> PIN3 root flower |
| title | PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root Elongation |
| title_full | PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root Elongation |
| title_fullStr | PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root Elongation |
| title_full_unstemmed | PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root Elongation |
| title_short | PIN3 from <i>Liriodendron</i> May Function in Inflorescence Development and Root Elongation |
| title_sort | pin3 from i liriodendron i may function in inflorescence development and root elongation |
| topic | <i>Liriodendron chinense</i> × <i>Liriodendron tulipifer</i> <i>Arabidopsis thaliana</i> PIN3 root flower |
| url | https://www.mdpi.com/1999-4907/13/4/568 |
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