Protocol to track the biosynthesis of cholesterol in cultured HCC cells using 13C compound-specific stable isotopic tracers

Protocol to track the biosynthesis of cholesterol in cultured HCC cells using 13C compound-specific stable isotopic tracers

Summary: Cholesterol biosynthesis supports proliferation and drives resistance to tyrosine kinase inhibitor (TKI) therapy in hepatocellular carcinoma (HCC). Here, we present a protocol for using stable isotopic tracers to track the biosynthesis of cholesterol in cultured HCC cells. We describe steps...

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Bibliographic Details
Main Authors: Jonathan D. Cybulski, Kit Sum Leung, Carmen Oi Ning Leung, David M. Baker, Terence Kin Wah Lee
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell Culture
Cancer
Metabolism
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004732

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