Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans
Abstract Background Clostridioides difficile is a major cause of antibiotic associated diarrhea. Several animal models are used to study C. difficile infection (CDI). The tree shrew has recently been developed as a model of primate processes. C. difficile infection has not been examined in tree shre...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-020-01943-z |
| _version_ | 1818885104783589376 |
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| author | Wenpeng Gu Wenge Li Wenguang Wang Dexuan Kuang Wenzhu Zhang Caixia Lu Na Li Pinfen Tong Yuanyuan Han Xiaomei Sun Jinxing Lu Yuan Wu Jiejie Dai |
| author_facet | Wenpeng Gu Wenge Li Wenguang Wang Dexuan Kuang Wenzhu Zhang Caixia Lu Na Li Pinfen Tong Yuanyuan Han Xiaomei Sun Jinxing Lu Yuan Wu Jiejie Dai |
| author_sort | Wenpeng Gu |
| collection | DOAJ |
| description | Abstract Background Clostridioides difficile is a major cause of antibiotic associated diarrhea. Several animal models are used to study C. difficile infection (CDI). The tree shrew has recently been developed as a model of primate processes. C. difficile infection has not been examined in tree shrews. We infected tree shrews with hyper-virulent C. difficile strains and examined the alterations in gut microbiota using 16S rRNA gene sequencing. Results C. difficile colonized the gastrointestinal tract of tree shrew and caused diarrhea and weight loss. Histopathologic examination indicated structures and mucosal cell destruction in ileal and colonic tissues. The gut microbial community was highly diversity before infection and was dominated by Firmicutes, Fusobacteria, Bacteroidetes, and Proteobacteria. Antibiotic administration decreased the diversity of the gut microbiota and led to an outgrowth of Lactobacillus. The relative abundance of Proteobacteria, Gammaproteobacteria, Enterobacteriales, Lachnospiraceae, Enterobacteriaceae, Escherichia, Blautia, and Tyzzerella increased following C. difficile infection. These taxa could be biomarkers for C. difficile colonization. Conclusions In general, the disease symptoms, histopathology, and gut microbiota changes following C. difficile infection in tree shrews were similar to those observed in humans. |
| first_indexed | 2024-12-19T16:00:08Z |
| format | Article |
| id | doaj.art-aece1bf673644cd09bff434360fcf9b8 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-19T16:00:08Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-aece1bf673644cd09bff434360fcf9b82022-12-21T20:14:57ZengBMCBMC Microbiology1471-21802020-08-0120111310.1186/s12866-020-01943-zResponse of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humansWenpeng Gu0Wenge Li1Wenguang Wang2Dexuan Kuang3Wenzhu Zhang4Caixia Lu5Na Li6Pinfen Tong7Yuanyuan Han8Xiaomei Sun9Jinxing Lu10Yuan Wu11Jiejie Dai12Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionCenter of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree ShrewAbstract Background Clostridioides difficile is a major cause of antibiotic associated diarrhea. Several animal models are used to study C. difficile infection (CDI). The tree shrew has recently been developed as a model of primate processes. C. difficile infection has not been examined in tree shrews. We infected tree shrews with hyper-virulent C. difficile strains and examined the alterations in gut microbiota using 16S rRNA gene sequencing. Results C. difficile colonized the gastrointestinal tract of tree shrew and caused diarrhea and weight loss. Histopathologic examination indicated structures and mucosal cell destruction in ileal and colonic tissues. The gut microbial community was highly diversity before infection and was dominated by Firmicutes, Fusobacteria, Bacteroidetes, and Proteobacteria. Antibiotic administration decreased the diversity of the gut microbiota and led to an outgrowth of Lactobacillus. The relative abundance of Proteobacteria, Gammaproteobacteria, Enterobacteriales, Lachnospiraceae, Enterobacteriaceae, Escherichia, Blautia, and Tyzzerella increased following C. difficile infection. These taxa could be biomarkers for C. difficile colonization. Conclusions In general, the disease symptoms, histopathology, and gut microbiota changes following C. difficile infection in tree shrews were similar to those observed in humans.http://link.springer.com/article/10.1186/s12866-020-01943-zClostridioides difficileTree shrewGut microbiota |
| spellingShingle | Wenpeng Gu Wenge Li Wenguang Wang Dexuan Kuang Wenzhu Zhang Caixia Lu Na Li Pinfen Tong Yuanyuan Han Xiaomei Sun Jinxing Lu Yuan Wu Jiejie Dai Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans BMC Microbiology Clostridioides difficile Tree shrew Gut microbiota |
| title | Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans |
| title_full | Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans |
| title_fullStr | Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans |
| title_full_unstemmed | Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans |
| title_short | Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans |
| title_sort | response of the gut microbiota during the clostridioides difficile infection in tree shrews mimics those in humans |
| topic | Clostridioides difficile Tree shrew Gut microbiota |
| url | http://link.springer.com/article/10.1186/s12866-020-01943-z |
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