Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil.
Visceral leishmaniasis (VL) has recently emerged in various urban and peri-urban areas of Brazil and other countries. Understanding the urbanization of VL requires identification of risk factors associated with human and canine infection. To determine the predictors of risk for canine VL, a survey w...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3750039?pdf=render |
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| author | Wendel Coura-Vital Alexandre Barbosa Reis Maria Arlene Fausto Gleisiane Gomes de Almeida Leal Marcos José Marques Vanja Maria Veloso Mariângela Carneiro |
| author_facet | Wendel Coura-Vital Alexandre Barbosa Reis Maria Arlene Fausto Gleisiane Gomes de Almeida Leal Marcos José Marques Vanja Maria Veloso Mariângela Carneiro |
| author_sort | Wendel Coura-Vital |
| collection | DOAJ |
| description | Visceral leishmaniasis (VL) has recently emerged in various urban and peri-urban areas of Brazil and other countries. Understanding the urbanization of VL requires identification of risk factors associated with human and canine infection. To determine the predictors of risk for canine VL, a survey was conducted of 1,443 dogs, from which a cohort was selected (n = 455) and evaluated for approximately 26 months. Serology was conducted with two enzyme-linked immunosorbent assays (ELISA): one conducted in the Laboratory of Zoonosis of the Belo Horizonte Health Department (LZOON) and the other in the Laboratory of Immunopathology of the Federal University of Ouro Preto (LIMP). A molecular diagnostic method (PCR-restriction fragment length polymorphism) and a structured questionnaire were also used. To identify the factors associated with seroconversion, two time-dependent Cox regression models were performed with different sensitivities (model 1, seroconversion by ELISA/LZOON; model 2, seroconversion by ELISA/LIMP). The overall incidences of seroconversion were 6.5/1000 dogs-months and 11.2/1000 dogs-months for ELISA/LZOON and ELISA/LIMP, respectively. Increased risk of seroconversion was associated with short fur (model 1: hazard ratio [HR] 1.9), the presence of dry leaves (model 1: HR 2.8) or manure (model 1: HR 3.5) in the backyard, dogs sleeping predominantly in the backyard (model 2: HR 2.1), the presence of symptoms (model 2: HR 2.0), and positive molecular results during follow-up (model 2: HR 1.5). Decreased risk was associated with insecticide spraying in the house (model 2: HR 0.5). These results indicate that more-vulnerable domiciles, certain dog behaviors, lack of vector control measures, and positive molecular results were associated with the occurrence of canine VL. Furthermore, it is important to emphasize that PCR-positive dogs should be monitored, owing to the possibility of seroconversion. Identifying risk factors for seroconversion in dogs is crucial for developing adequate strategies for VL prevention and control. |
| first_indexed | 2024-12-11T18:16:31Z |
| format | Article |
| id | doaj.art-aecfcd24e8774b3e869b90a271742565 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T18:16:31Z |
| publishDate | 2013-01-01 |
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| spelling | doaj.art-aecfcd24e8774b3e869b90a2717425652022-12-22T00:55:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7183310.1371/journal.pone.0071833Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil.Wendel Coura-VitalAlexandre Barbosa ReisMaria Arlene FaustoGleisiane Gomes de Almeida LealMarcos José MarquesVanja Maria VelosoMariângela CarneiroVisceral leishmaniasis (VL) has recently emerged in various urban and peri-urban areas of Brazil and other countries. Understanding the urbanization of VL requires identification of risk factors associated with human and canine infection. To determine the predictors of risk for canine VL, a survey was conducted of 1,443 dogs, from which a cohort was selected (n = 455) and evaluated for approximately 26 months. Serology was conducted with two enzyme-linked immunosorbent assays (ELISA): one conducted in the Laboratory of Zoonosis of the Belo Horizonte Health Department (LZOON) and the other in the Laboratory of Immunopathology of the Federal University of Ouro Preto (LIMP). A molecular diagnostic method (PCR-restriction fragment length polymorphism) and a structured questionnaire were also used. To identify the factors associated with seroconversion, two time-dependent Cox regression models were performed with different sensitivities (model 1, seroconversion by ELISA/LZOON; model 2, seroconversion by ELISA/LIMP). The overall incidences of seroconversion were 6.5/1000 dogs-months and 11.2/1000 dogs-months for ELISA/LZOON and ELISA/LIMP, respectively. Increased risk of seroconversion was associated with short fur (model 1: hazard ratio [HR] 1.9), the presence of dry leaves (model 1: HR 2.8) or manure (model 1: HR 3.5) in the backyard, dogs sleeping predominantly in the backyard (model 2: HR 2.1), the presence of symptoms (model 2: HR 2.0), and positive molecular results during follow-up (model 2: HR 1.5). Decreased risk was associated with insecticide spraying in the house (model 2: HR 0.5). These results indicate that more-vulnerable domiciles, certain dog behaviors, lack of vector control measures, and positive molecular results were associated with the occurrence of canine VL. Furthermore, it is important to emphasize that PCR-positive dogs should be monitored, owing to the possibility of seroconversion. Identifying risk factors for seroconversion in dogs is crucial for developing adequate strategies for VL prevention and control.http://europepmc.org/articles/PMC3750039?pdf=render |
| spellingShingle | Wendel Coura-Vital Alexandre Barbosa Reis Maria Arlene Fausto Gleisiane Gomes de Almeida Leal Marcos José Marques Vanja Maria Veloso Mariângela Carneiro Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil. PLoS ONE |
| title | Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil. |
| title_full | Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil. |
| title_fullStr | Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil. |
| title_full_unstemmed | Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil. |
| title_short | Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil. |
| title_sort | risk factors for seroconversion by leishmania infantum in a cohort of dogs from an endemic area of brazil |
| url | http://europepmc.org/articles/PMC3750039?pdf=render |
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